@ARTICLE{Karnwal_Arun_Screening,_2019,
 author={Karnwal, Arun},
 volume={vol. 59},
 number={No 3},
 journal={Journal of Plant Protection Research},
 pages={293–303},
 howpublished={online},
 year={2019},
 publisher={Committee of Plant Protection PAS},
 publisher={Institute of Plant Protection – National Research Institute},
 abstract={Globally more than 5.2 billion hectares of farming fields are damaged through erosion, salinity and soil deterioration. Many salt stress tolerant bacteria have plant growth promoting (PGP) characteristics that can be used to overcome environmental stresses. Isolation and screening of salt-tolerant endophytes from Salicornia brachiata were achieved through surface sterilization of leaves followed by cultivation on 4% NaCl amended media. Performance of isolates towards indole-3-acetic acid (IAA) production, phosphate solubilization, ACC deaminase activity, ammonia production, siderophore production and stress tolerance were determined. On the basis of the highest plant growth promoting activity, SbCT4 and SbCT7 isolates were tested for plant growth promotion with wheat and maize crops. In the present study, a total of 12 morphologically distinct salt-tolerant endophytic bacteria was cultured. Out of 12 isolates, 42% of salt-tolerant endophytes showed phosphate solubilization, 67% IAA production, 33% ACC-deaminase activity, 92% siderophore production, 41.6% ammonia production and 66% HCN production. A dendrogram, generated on the basis of stress tolerance, showed two clusters, each including five isolates. The bacterial isolates SbCT4 and SbCT7 showed the highest stress tolerance, and stood separately as an independent branch. Bacterial isolates increased wheat shoot and root dry weights by 60–82% and 50–100%, respectively. Similarly, improved results were obtained with maize shoot (27–150%) and root (80–126%) dry weights. For the first time from this plant the bacterial isolates were identified as Paenibacillus polymyxa SbCT4 and Bacillus subtilis SbCT7 based on phenotypic features and 16S rRNA gene sequencing. Paenibacillus polymyxa SbCT4 and B. subtilis SbCT7 significantly improved plant growth compared to non-inoculated trials.},
 type={Article},
 title={Screening, isolation and characterization of culturable stress-tolerant bacterial endophytes associated with Salicornia brachiata and their effect on wheat (Triticum aestivum L.) and maize (Zea mays) growth},
 URL={http://czasopisma.pan.pl/Content/113253/PDF/01_OA9_JPPR_59_3_349_Karnwal.pdf},
 doi={10.24425/jppr.2019.129747},
 keywords={endophyte, MEGA X, plant growth promotion, Salicornia brachiata, salt stress},
}