TY - JOUR N2 - Perinatal calf mortality in dairy herds has been reported worldwide. The etiology of stillbirth is multifactorial, and can be caused by various species of bacteria and environmental factors. Among them some potential pathogens from the Mollicutes class such as Mycoplasma (M.) spp. and Ureaplasma (U.) diversum can be isolated from the bovine genital tract and other organs of the suspected cattle. The aim of this study was to evaluate if the bacteria belonging to the Molli- cutes class i.e. M. bovis, M. bovigenitalium, M. canadense, M. canis, M. arginini, M. bovirhinis, M. dispar, M. alkalescens and U. diversum could have an impact on perinatal calf mortality in selected Polish dairy farms. The material was: 121 stillborn calves (SB), 21 live born calves (C) and 131 cows (dams) from 30 Polish Holstein-Friesian herds. Samples were examined from all the SB calves’ and six control euthanized calves’ abomasal contents and lung samples collected during necropsy, and from the dams’ serum and placenta. In dams the serological ELISA, and in calves and placenta samples molecular PCR/denaturing gradient gel electrophoresis, methods were used. Screening of dams’ sera for antibodies to M. bovis (ELISA) showed seven dams positive for M. bovis, whereas none of the nine examined Mollicutes microorganisms were detected in the placenta and calves. L1 - http://czasopisma.pan.pl/Content/106888/PDF/03.pdf L2 - http://czasopisma.pan.pl/Content/106888 PY - 2018 IS - No 3 EP - 444 DO - 10.24425/122616 KW - perinatal mortality KW - stillborn calves KW - Mollicutes KW - serology KW - PCR/DGGE A1 - Szacawa, E. A1 - Jawor, P. A1 - Dudek, K. A1 - Bednarek, D. A1 - Stefaniak, T. PB - Polish Academy of Sciences Committee of Veterinary Sciences PB - University of Warmia and Mazury in Olsztyn VL - vol. 21 DA - 2018.11.20 T1 - Screening for Mollicutes microorganisms in perinatal calf mortality cases in Polish dairy herds SP - 441 UR - http://czasopisma.pan.pl/dlibra/publication/edition/106888 T2 - Polish Journal of Veterinary Sciences ER -