TY - JOUR N2 - αB-crystallin is a member of a small family of thermal shock proteins that protects cells from stress. Because of lack of its expression in peripheral blood leukocytes, it was proposed as a molecular marker of circulating tumor cells in canine mammary gland tumors. The aim of the present study was to determine if αB-crystallin shows stability of expression, what is the requirement for this type of marker. It was also assessed whether there is co-expression of αB-crystallin with the basal marker, cytokeratin 17. For this purpose, samples of various types of canine mammary gland tumors of epithelial origin, were selected. Using RT-qPCR, we have found αB-crystallin and cytokeratin 17 co-expression in benign and malignant canine mammary gland tumors. It has been demonstrated that the expression of αB-crystallin in tested neoplastic samples is not stable in comparison to the control group. Furthermore αB-crystallin overor down- expression was associated witch the same cytokeratin 17 pattern. αB-crystallin can be a marker of circulating tumor cells in the bloodstream, but for cancers in which basal marker expression occurs and thus not universal for all cancers originating from the mammary gland tissue. L1 - http://czasopisma.pan.pl/Content/113456/PDF/11.pdf L2 - http://czasopisma.pan.pl/Content/113456 PY - 2019 IS - No 3 EP - 529 DO - 10.24425/pjvs.2019.129960 KW - αB-crystallin (CRYAB) KW - canine mammary gland tumor KW - circulating tumor cells (CTC) KW - cytokeratin 17 (CK17) A1 - Chmielewska-Krzesińska, M. A1 - Jakimiuk, A. A1 - Wąsowicz, K. PB - Polish Academy of Sciences Committee of Veterinary Sciences PB - University of Warmia and Mazury in Olsztyn VL - vol.22 DA - 2019.09.24 T1 - Stability of αB-crystallin gene expression in canine mammary gland neoplasms. Should it be considered as circulating tumor cell genetic marker? SP - 523 UR - http://czasopisma.pan.pl/dlibra/publication/edition/113456 T2 - Polish Journal of Veterinary Sciences ER -