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Słowa kluczowe mycotoxins toxicity comet assay
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Abstrakt

The aim of this study was to reveal the potentially genotoxic effect of zearalenone on bovine lymphocytes by comet assay in vitro. The bovine lymphocytes were exposed to various zearalenone concetrations (50; 10; 2; 0.4 and 0.08 ppm). The viability and DNA damage of lymphocytes was monitored after 2 h, 24 h, 48 h and 72 h. After 2 hours of zearalenone exposure, statistically significant DNA damage occurred at all tested concentrations of 0.08 ppm (12.2±1.25; p<0.05), 0.4 ppm (12.7±0.88; p<0.01), 2 ppm (12.0±0.51; p<0.01), 10 ppm (11.2±0.47; p<0.01) and at 50 ppm (14.2±0 61; p<0.001). Significantly greater DNA damage was also found after 24 h, 48 h and 72 h. The obtained results showed that zearalenone may induce DNA damage of the bovine lymphocytes.
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Bibliografia


Bouaziz C, Bouslimi A, Kadri R, Zaied C, Bacha H, Abid-Essefi S (2013) The in vitro effects of zearalenone and T-2 toxins on Vero cells. Exp Toxicol Pathol 65: 497-501.
Collins AR (2004) The comet assay for DNA damage and repair. Principles, applications, and limitations. Mol Biotechnol 26: 249-261.
Gao F, Jiang LP, Chen M, Geng CY, Yang G, Ji F, Zhong LF (2013) Genotoxic effects induced by zearalenone in a human embryonic kidney cell line. Mutat Res 755: 6-10.
Jackson SP (2002) Sensing and repairing DNA double-strand breaks. Carcinogenesis 23: 687-696.
Kachlek M, Szabó-Fodor J, Blochné Bodnár Z, Horvatovich K, Kovács M (2017) Preliminary results on the interactive effects of deoxynivalenol, zearalenone and fumonisin B1 on porcine lymphocytes. Acta Vet Hung 65: 340-353.
Klarić MŠ, Daraboš D, Rozgaj R, Kašuba V, Pepeljnjak S (2010) Beauvericin and ochratoxin A genotoxicity evaluated using the alkaline comet assay: single and combined genotoxic action. Arch Toxicol 84: 641-650.
Koleničová S, Holečková B, Galdíková M, Schwarzbacherová V, Drážovská M (2021) Genotoxicity testing of bovine lymphocytes exposed to epoxicon-azole using alkaline and neutral comet assay. Caryologia 11: 99-109.
Końca K, Lankoff A, Banasik A, Lisowska H, Kuszewski T, Góźdź S, Koza Z, Wojcik A (2003) A cross-platform public domain PC im-age-analysis program for the comet assay. Mutat Res 534: 15-20.
Marin DE, Taranu I, Burlacu R, Manda G, Motiu, M, Neagoe I, Dragomir C, Stancu, M, Calin L (2011) Effects of zearalenone and its deriva-tives on porcine immune response. Toxicol In Vitro 25: 1981-1988.
Reddy KE, Song J, Lee HJ, Kim M, Kim DW, Jung HJ, Kim B, Lee Y, Yu D, Kim DW, Oh YK, Lee SD (2018) Effects of high levels of deoxynivalenol and zearalenone on growth performance, and hematological and immunological parameters in pigs. Toxins (Basel) 10: 114.
Szabo RT, Kovács-Weber M, Erdélyi M, Balogh K, Fazekas N, Horváth Á, Mézes M, Kovács B (2019) Comet assay study of the genotoxic effect of T-2 and HT-2 toxins in chicken hepatocytes. Biol Fut 70: 330-335.
Thapa A, Horgan KA, White B, Walls D (2021) Deoxynivalenol and zearalenone-synergistic or antagonistic agri-food chain co-contaminants? Toxins (Basel) 13: 561.
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Autorzy i Afiliacje

M. Harcarova
1
E. Conkova
2
S. Kolenicova
3
B. Holeckova
3
M. Proskovcova
2

  1. Department of Animal Nutrition and Husbandry, University of Veterinary Medicine and Pharmacy in Kosice, Komenskeho 73, 041 81 Kosice, Slovak Republic
  2. Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy in Kosice, Komenskeho 73, 041 81 Kosice, Slovak Republic
  3. Department of Biology and Physiology, University of Veterinary Medicine and Pharmacy in Kosice, Komenskeho 73, 041 81 Kosice, Slovak Republic
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Abstrakt

The present study was conducted to evaluate the effect of humic substances on performance and selected blood biochemical parameters in turkeys. A total of twenty 6-week-old turkey hybrids (Big 6) were divided into two groups. The first group of turkeys was fed the basal diet without any supplementation of humic substances as a control group. The second group was fed the basal diet supplemented with 5 g of humic substances per kg of diet. The study lasted 35 days, until the 11th week of age of the turkeys. The addition of humic substances had no effect on the live body weight, body weight gain, feed intake, feed conversion ratio, or growth rate of turkeys. Similarly, the monitored blood biochemical parameters, except for the levels of P, Mg, and Na, were not significantly affected by the addition of humic substances. The addition of humic substances led to a significant decrease in the concentration of P (p˂0.05), Mg (p˂0.05), and Na (p˂0.01) in the blood serum of turkeys. It was concluded that the addition of humic substances may lead to a reduction of some mineral substances in the blood of animals, which may be due to their chelating effects. This may indicate a reduced availability of some biologically important minerals for the body, which could subsequently manifest itself in some health issues and a decrease in animal production. During the duration of our study, there were no signs of disturbance in the health of the animals. In view of the achieved results, further studies will be necessary to determine the appropriate concentration and duration of administration of the monitored preparation and to evaluate its influence on the availability of nutrients and feed conversion and its possible use as a safe non-antibiotic growth stimulator in turkey nutrition.
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Bibliografia

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Agina OA, Ezema WS, Nwishienyi CN (2015) Haemato-biochemical profile of apparently healthy domestic turkeys (Meleagris gallopavo) in Nsukka, Enugu state, Nigeria. Anim Res Int 12: 2120-2129.

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Hudák M, Semjon B, Marcinčáková D, Bujňák L, Naď P, Koréneková B, Nagy J, Bartkovský M, Marcinčák S (2021) Effect of broilers chicken diet supplementation with natural and acidified humic substances on quality of produced breast meat. Animals 11: 1087.

Ipek H, Avci M, Iriadam M, Kaplan O, Denek N (2008) Effects of humic acid on some hematological parameters, total antioxidant capacity and laying performance in Japanese quails. Arch Geflugelk 72: 56-60.

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Autorzy i Afiliacje

Hreško Šamudovská
1
L. Bujňák
1
A. Marcin
1
T. Mihok
1
M. Harčárová
1
L. Zábranský
2
P. Naď
1

  1. Department of Animal Nutrition and Husbandry, University of Veterinary Medicine and Pharmacy in Kosice, Komenskeho 73, 041 81 Kosice, Slovak Republic
  2. Department of Animal Husbandry Sciences, Faculty of Agriculture and Technology, University of South Bohemia in Ceske Budejovice, Studentska 1668, 370 05 Ceske Budejovice, Czech Republic

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