Considering the low accuracy and low efficiency of the traditional calibration method for base strain sensitivity of accelerometers, a novel base strain sensitivity calibration system with steady harmonic excitation is proposed. The required cantilever beam for calibration is driven by an electromagnetic exciter to generate a base strain varying in a steady harmonic pattern. By applying a Wheatstone bridge circuit, the generated strain with low distortion can be measured. The measurement system with a compensation function can automatically calibrate the base strain sensitivity. The amplitude linearity and frequency response characteristics of the base strain sensitivity in two accelerometers are obtained experimentally, and the uncertainty in the results is 2% ( k = 2).