In the study we have focused on the distribution of several metals (Cr, As, Pb) and anions (Cr, NO3-) and their partition between pore (interstitial) waters and sediments sampled at three stations at the Dobczyce Reservoir which supplies the drinking water to inhabitants from the city of Kraków and its agglomeration. The results show considerable increase in concentrations of Pb and As in pore water samples, when compared to the bottom waters. Meaningful alternations in concentration were observed in case of pore water samples (Pb, As) and sediments (Cr, Pb, As), coming from three stations and their lateral sections. The possible relations between this phenomenon and the sediment characteristic as well as the Fe and Ca content, has been studied. Some comments on the seasonal variations of anion contents in water and pore water samples, are also provided. It was found that nitrates and sulphates show considerable variations.

Cultivation-based assays represent the gold standard for the assessment of virus infectivity; however, they are time-consuming and not suitable for every virus type. Pre-treatment with platinum (Pt) compounds followed by real-time PCR has been shown to discriminate between infectious and non-infectious RNA viruses. This study examined the effect of Pt and palladium (Pd) compounds on enveloped DNA viruses, paying attention to two significant pathogens of livestock – bovine herpesvirus-1 (BoHV-1) and African swine fever virus (ASFV). Native or heat-treated BoHV-1 suspension was incubated with the spectrum of Pt/Pd compounds. Bis(benzonitrile)palladium(II) dichloride (BB-PdCl ₂) and dichloro(1,5-cyclooctadiene) palladium(II) (PdCl ₂-COD) produced the highest differences found between native and heat- -treated viruses. Optimized pre-treatment conditions (1 mM of Pd compound, 15 min, 4°C) were applied on both virus genera and the heat inactivation profiles were assessed. A significant decrease in the detected quantity of BoHV-1 DNA and ASFV DNA after heat-treatment (60°C and 95°C) and consequent incubation with Pd compounds was observed. BB-PdCl ₂ and PdCl ₂-COD could help to distinguish between infectious and non-infectious enveloped DNA viruses such as BoHV-1 or ASFV.