This study investigated the quantity and distribution of extracellular polymeric substances (EPS) in aerobic granules. Results showed that EPS play an important role in the formation and stabilisation of granules. The content of EPS significantly increases during the first weeks of biogranulation. An analysis of EPS in the granules revealed that the protein level was 5 times higher than in polysaccharides. The increase of protein content correlated with the growth of cell hydrophobicity (r2 = 0.95). EPS and hydrophobicity are important factors in cell adhesion and formation of granules.

The aim of this work was also to determine the distribution of EPS in the granule structure. In situ EPS staining showed that EPS are located mostly in the center of granules and in the subsurface layer. The major components of the EPE matrix are proteins, nucleic acids and β-polysaccharides. These observations confirm the chemical extraction data and indicate that granule formation and stability are dependent on protein content.

Three synchronous machine models representing three precision levels (complete, reduced and static), implemented in a virtual synchronous generator (VSG)-based industrial inverter, are compared and discussed to propose a set of tests for a possible standardization of VSG-based inverters and to ensure their “grid-friendly” operation in the context of isolated microgrids. The models and their implementation in the microcontroller of an industrial inverter (with the local control) are discussed, including the usability of the implementation with large-scale developments constraints in mind. The comparison is conducted based on existing standards (for synchronous machines and diesel generators) in order to determine their needed evolution, to define the requirements for future grid-friendly inverter-based generators, notably implementing a VSG solution.