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Abstract

Purpose: To assess the initial therapy of chronic superficial keratitis (CSK) in dogs with the use of dexamethasone and cyclosporine/ dimethyl sulfoxide combination eye drops.
Methods: The study was conducted on 41 dogs – 16 males and 25 females, aged 2 to 9 years, diagnosed with CSK. The disease was treated with two kinds of eye drops containing 0.1% dexamethasone and 0.75% cyclosporine in combination with 30% DMSO, administered three times a day. Prior to the treatment and after 5 weeks of therapy, depigmentation of the third eyelid margin, corneal neovascularization and pigmentation were assessed. The percentage of the corneal surface afflicted with inflammatory processes was calculated with the use of IsoCalc.com’s Get Area software for CorelDRAW12.
Results: The administered therapy resulted in a significant decrease in the mean number of quadrants affected by corneal neovascularization in the right eye from 2.63 prior to treatment to 0.24 after treatment (p<0.001), and the left eye from 2.66 to 0.59 (p<0.001), respectively. Mean corneal surface afflicted with inflammatory processes was statistically significantly reduced from 53.5% to 26.3% (p<0.001) in the case of right corneas, and from 54.5% to 30.2% (p<0.001) in the case of left corneas. Of 77 corneas diagnosed with pigmentation, pigmentation reduction was observed in 54 and pigmentation increase in 27.
Conclusions: Using dexamethasone and cyclosporine/DMSO combination eye drops proved to be a viable initial therapy against CSK, which facilitates reduction of inflammatory processes and neovascularization atrophy, but in many cases does not inhibit the progress of pigmentation.
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Bibliography


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Denk N, Fritsche J, Reese S (2011) The effect of UV-blocking contact lenses as a therapy for canine chronic superficial keratitis. Vet Ophthalmol 14: 186-194.
Jokinen P, Rusanen EM, Kennedy LJ, Lohi H (2011) MHC class II risk haplotype associated with canine chronic superficial keratitis in German Shepherd dogs. Vet Immunol Immunopathol 140: 37-41.
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Authors and Affiliations

I. Balicki
1
M. Szadkowski
1
A. Balicka
2
J. Zwolska
1

  1. Department and Clinic of Animal Surgery, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Poland
  2. Small Animal Clinic, University of Veterinary Medicine and Pharmacy in Košice, Slovakia
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Abstract

The aim of the study was to determine the thickness of choroidal layers in mixed breed dogs suffering from retinal atrophy (RA) and showing symptoms of progressive retinal atrophy (PRA), with the use of SD-OCT. The study was performed on 50 dogs divided into two groups: 25 dogs diagnosed with retinal atrophy (RA) with PRA symptoms aged 1.5-14 years and 25 healthy dogs aged 2-12 years. The dogs were examined using slit-lamp biomicroscopy, tonometry, ophthalmoscopy, fundus camera and SD-OCT (Topcon 3D OCT 2000). Measurements of the choroidal layers: RPE-Bruch membrane-choriocapillaris complex (RPE-BmCc) with tapetum lucidum in tapetal fundus, medium-sized vessel layer, (MSVL), large vessel layer with lamina suprachoroidea and (LVLS) whole choroidal thickness (WCT) were taken manually with the use of the caliper function integrated with the SD-OCT software. The measurements were performed dorsally (D) and ventrally (V) at a distance of 5000-6000 μm, and temporally (T) and nasally (N) at a distance of 4000-7000 μm from the optic disc with enhanced depth scans. The measurements were conducted temporally and nasally both in the tapetal (TempT, Nast) and nontapetal (TempNT, NasNT) fundus. Statistical analysis was performed using Statistica 10 software (Mann Whitney U Test). In all dogs affected by retinal atrophy (RA) with PRA symptoms, a statistically significant (p≤0,05) reduction in thickness of MSVL was observed in all the measured regions. A statistically significant reduction in thickness of LVLS and WCT was found in all nontapetal areas (p≤0,05). RA in mixed breed dogs with PRA symptoms was accompanied by choroid disorders such as reduction in thickness of the large vessel layer and decreased whole choroidal thickness in the nontapetal fundus as well as the medium vessel layer in all fundus regions.
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Authors and Affiliations

J. Zwolska
1
I. Balicki
1
A. Balicka
2
B. Kuduk
1

  1. Department and Clinic of Animal Surgery, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30, 20-612 Lublin, Poland
  2. Small Animals Clinic,University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 040 01 Košice, Slovakia
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Abstract

Among the technologies which allow to reduce greenhouse gas emission, mainly carbon dioxide, special attention deserves the idea of 'zero-emission' technology based on boilers working in oxy-combustion technology. In the paper the results of analyses of the influence of changing two quantities, namely oxygen share in oxidant produced in the air separation unit, and oxygen share in oxidant supplied to the furnace chamber on the selected characteristics of a steam boiler including the degree of exhaust gas recirculation, boiler efficiency and adiabatic flame temperature, was examined. Due to the possibility of the integration of boiler model with carbon dioxide capture, separation and storage installation, the subject of the analysis was also to determine composition of the flue gas at the outlet of a moisture condensation installation. Required calculations were made using a model of a supercritical circulating fluidized bed boiler working in oxy-combustion technology, which was built in a commercial software and in-house codes.

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Authors and Affiliations

Adrian Balicki
Łukasz Bartela
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Abstract

In this paper an air separation unit was analyzed. The unit consisted of: an ionic transport membrane contained in a four-end type module, an air compressor, an expander fed by gas that remains after oxygen separation and heat exchangers which heat the air and recirculated flue gas to the membrane operating temperature (850 °C). The air separation unit works in a power plant with electrical power equal to 600 MW. This power plant additionally consists of: an oxy-type pulverized-fuel boiler, a steam turbine unit and a carbon dioxide capture unit. Life steam parameters are 30 MPa/650 °C and reheated steam parameters are 6 MPa/670 °C. The listed units were analyzed. For constant electrical power of the power plant technical parameters of the air separation unit for two oxygen recovery rate (65% and 95%) were determined. One of such parameters is ionic membrane surface area. In this paper the formulated equation is presented. The remaining technical parameters of the air separation unit are, among others: heat exchange surface area, power of the air compressor, power of the expander and auxiliary power. Using the listed quantities, the economic parameters, such as costs of air separation unit and of individual components were determined. These quantities allowed to determine investment costs of construction of the air separation unit. In addition, they were compared with investment costs for the entire oxy-type power plant.

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Authors and Affiliations

Janusz Kotowicz
Sebastian Michalski
Adrian Balicki
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Abstract

The paper analyzes a new concept of integration of combined cycle with the installation of supplementary firing. The whole system was enclosed by thermodynamic analysis, which consists of a gas-steam unit with triple-pressure heat recovery steam generator. The system uses a determined model of the gas turbine and the assumptions relating to the construction features of steam-water part were made. The proposed conception involves building of supplementary firing installation only on part of the exhaust stream leaving the gas turbine. In the proposed solution superheater was divided into two sections, one of which was located on the exhaust gases leaving the installation of supplementary firing. The paper presents the results of the analyses of which the main aim was to demonstrate the superiority of the new thermodynamic concept of the supplementary firing over the classical one. For this purpose a model of a system was built, in which it was possible to carry out simulations of the gradual transition from a classically understood supplementary firing to the supplementary firing completely modified. For building of a model the GateCycle™ software was used.

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Authors and Affiliations

Janusz Kotowicz
Łukasz Bartela
Adrian Balicki
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Abstract

Among the technologies which allow to reduce greenhouse gas emissions, mainly of carbon dioxide, special attention deserves the idea of 'zero-emission' technology based on boilers working in oxy-combustion technology. In the paper a thermodynamic analysis of supercritical power plant fed by lignite was made. Power plant consists of: 600 MW steam power unit with live steam parameters of 650°C/30 MPa and reheated steam parameters of 670°C/6 MPa; circulating fluidized bed boiler working in oxy-combustion technology; air separation unit and installation of the carbon dioxide compression. Air separation unit is based on high temperature membrane working in three-end technology. Models of steam cycle, circulation fluidized bed boiler, air separation unit and carbon capture installation were made using commercial software. After integration of these models the net electricity generation efficiency as a function of the degree of oxygen recovery in high temperature membrane was analyzed.
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Authors and Affiliations

Janusz Kotowicz
Adrian Balicki
Sebastian Michalski
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Abstract

Calretinin (CR), a calcium-binding protein from EF-hand family, is localised in non-pyramidal GABA-ergic interneurons of the hippocampus. CR takes part in maintaining calcium binding homeostasis, which suggests its neuroprotective role. Hippocampal neurons contain membrane transient receptor potential vanilloid 1 (TRPV1) which binds to capsaicin (CAP) contained in habanero pepper fruits. Few in vivo studies have revealed the effect of CAP on interneurons containing CR. The aim of the present study was to investigate the CR immunoreac- tivity in interneurons of the hippocampal CA1 field and dentate gyrus (DG) in adult rats after intragastric admin- istration of the habanero pepper fruits. Wistar rats received a peanut oil – control group (C), and oil suspension of habanero pepper fruits at doses of 0.025 g dm/kg b.w. – group I and 0.08 g dm/kg b.w. – group II for 28 days. After euthanasia, the brains were collected and embedded in paraffin blocks using a routine histological tech- nique. Frontal hippocampal sections were immunohistochemically stained for CR by using a peroxidase-antiper- oxidase method. CR immunoreactive (CR-IR) interneurons were morphologically and morphometrically ana- lyzed under a light microscope. The results showed similar shapes and distribution of cells in both areas of the brain in group C and I of animals. However, CR-IR interneurons in the hippocampal CA1 field and in DG were occasionally observed in the group II of rats.

The results of morphometric studies did not reveal statistically significant differences in the surface area and shape index of cells between examined brain regions from groups I and II compared to group C.

Only in group II of rats, an increase in the digital immunostaining intensity of CR-IR interneurons was found in DG. Low number of CR-IR interneurons in the hippocampal CA1 field and in the DG, under the influence of a large dose of habanero pepper fruits containing CAP, may be caused by the activation of TRPV1 receptors and the increase in Ca2+ ions in these cells. This phenomenon may ultimately lead to neuronal death and may disturb neuronal conduction.

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Authors and Affiliations

J. Jaworska-Adamu
A. Krawczyk
K. Rycerz
M. Gołyński
A. Wawrzyniak
K. Lutnicki
I. Balicki

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