Disinfectants are commonly used in households, hospitals, in drug manufacturing, in food processing. With the ever-increasing antibiotic-resistance of microorganisms, it is crucial to rationally apply disinfectants in suitable concentrations, with proper active substance, as not all substances affect various organisms in the same way. Among the microorganisms that are particularly difficult to kill, there are bacteria producing spores - forms that have different structure and sensitivity to disinfectants than the vegetative forms. The aim of the study has been to examine the influence of frequently used disinfectant compounds upon the spores of bacteria of the Bacillus genus: B. cereus, B. mycoides, and B. subtilis. In the study of disinfectants the findings showed that the disinfectants with the best results against spores are: peracetic acid, hydrogen peroxide in concentrations of 30% as well as 5%, and Lysoformin 3000. The least efficient in fighting spores proved to be Isopropanol and Promanum N. Differentiation has been found to exist in the reaction of specific species to the preparation Rafasept, as B. subtilis occurred to be very sensitive to that compound, whereas Rafasept turned out to be ineffective in the case of B. mycoides and B. cereus.
This article concerns church monuments, which are part of the cultural heritage of Dukla. This is a town with medieval origins, located within the Podkarpackie Voivodeship, in the Krosno district. In the past it was an important centre of commerce on the Hungarian Route. Merchant traditions in Dukla are mainly associated with trade and storage of wine, which was kept in the cellars under the main market square in Dukla. Because of the rank, which the town used to have in the past, it can boast numerous monuments including objects associated with the administrative, commercial and economic functions of the town, as well as — or perhaps primarily — sacred objects. Among the latter, one has to mention firstly the parish church of St Mary Magdalene, the church and monastery complex of the Bernardine Order, and the synagogue of the Jewish community, that once used to live in Dukla. Those objects, their cultural value and issues related to their protection constitute the subject of this study.
The prevalence of heavy metals in wastewater is the cause of death of numerous organisms which take part in biological treatment of wastewater, that is why the aim of the study has been to asses the influence of cadmium and copper ions upon the microfauna of activated sludge. 5, 10, 50, and 100 mg/l of Cd2+ and Cu2+ were added into the samples of activated sludge and then, after 24 hours, the microscopic observations of activated sludge microfauna were carried out, and all changes concerning the amount of microfauna, functional groups, and species composition were determined. The results obtained allowed to find a high level of toxicity of Cd2+ and Cu2+ ions to activated sludge microfauna, which resulted in the changes in the value of the Sludge Biotic Index and classes of sludge, survivability of microfauna, and reduction in the number of taxonomic units. It was observed that Cu2+ ions are more toxic to activated sludge microfauna than Cd2+ ions in identical doses. Organisms sensitive to Cd2+ and Cu2+ ions have been found to be testate amoebae, Aspidisca sp. and Epistylis sp., as well as organisms relatively sensitive to tested metals, which turned out to be ciliates of Opercularia and Vorticella convalaria genera.
Experimental modal analysis of a violin with three different tensions of a bass bar has been performed. The bass bar tension is the only intentionally introduced modification of the instrument. The aim of the study was to find differences and similarities between top plate modal parameters determined by a bass bar perfectly fitting the shape of the top plate, the bass bar with a tension usually applied by luthiers (normal), and the tension higher than the normal value. In the modal analysis four signature modes are taken into account. Bass bar tension does not change the sequence of mode shapes. Changes in modal damping are insignificant. An increase in bass bar tension causes an increase in modal frequencies A0 and B(1+) and does not change the frequencies of modes CBR and B(1-).
In this paper, the influence of biomodification of recycled concrete aggregate (RCA) on some properties of concrete was studied. Sporosarcina pasteurii strain was chosen for biodeposition process. The RCA came from parent concretes with varying w/c ratio. Recycled aggregate concrete (RAC) with two levels of w/c ratio, made from RCA not subjected to biomodification, was treated as reference. Compressive strength, water absorption and sorption of concretes were tested. The most significant influence of the aggregate biomodification was found in the case of sorption and this effect was highest for RAC made from the aggregate yielding from better quality parent concrete.
This study characterizes mycorrhiza helper bacteria (MHB) from selected unpolluted locations as well as subjected to industrial emissions. To determine the species of bacteria isolated from the roots of ectomycorrhizal pine and birch, a method based on the sequence analysis of a 16S rRNA gene was used. The isolated bacteria were initially characterized by available biochemical methods and phenotypic observation. On the selected bacteria representatives isolation of DNA was performed, on which the PCR reaction was carried out. In this way amplified samples were automatically sequenced and the obtained results were compared to public databases. Among the isolated bacteria Pseudomonas fluorescens SBW25 and Burkholderia xenovorans LB400 species were dominant.
The article presents the issue related with a proper preparation of a data sheet for the
analysis, the way of verifying the correctness and reliability of input information, and proper
data encoding. Improper input or coding of data can significantly influence the correctness
of performed analyses or extend their time. This stage of an analysis is presented by an
authorship questionnaire for the study on occupational safety culture in a manufacturing
plant, using the Statistica software for analyses. There were used real data, obtained during
the research on the issue of occupational safety and factors having the greatest influence on
the state of occupational safety.