It has long been observed that toxic heavy metals at different concentrations can induce root hair development in plants. In oilseed rape we studied ethylene levels and root hair initiation under Cd2+ stress. Growth of the primary root was inhibited but close to root tips the development of subapical root hairs was significantly stimulated by Cd2+ at 30 μM. Versus the control, the distance between the root tip and the root hair zone and the length of the epidermal cell in the elongation zone were significantly reduced by Cd2+ at the same concentration. Exogenous application of Cd2+ and 1-aminocyclopropane-1-carboxylate (ACC) to roots had similar effects on subapical root hair development. Hair density increase and hair elongation in the presence of Cd2+ were reduced by the ethylene inhibitors CoCl2 at 15 μM and aminooxyacetic acid (AOA) at 10 μM. Exposing roots to Cd2+ caused a rapid increase in superoxide radical (O2 ·-) production in the root hair differentiation zone, and at the tips of emerging and newly formed root hairs. Cd2+-induced O2 ·- production at the growing hair tips was blocked in the presence of AOA. Our findings suggest that Cd2+-induced ethylene signaling may act upstream of O2 ·-. Cd2+ promotion of O2 ·- production may operate through an ethylene signaling pathway, and O2 ·- itself may stimulate root hair elongation.
A kind of generalized proportional-integral(GPI) observer for descriptor linear systems is introduced. We first propose two complete parametric solutions to generalized Sylvester matrix equation corresponding to the left eigenvector matrices in the case of Jordan form. Then a parametric design approach for the observer is presented. The proposed method provides all parametric expression of the gain matrices and the corresponding finite left eigenvector matrix and guarantees the regularity and impulse-freeness of the expanded error system. Two numerical examples are given to explain the design procedure and illustrate the effectiveness of the proposed method.
The parenchyma cellulose isolated from bagasse pith was used as an alternative resource for preparation of water-soluble cellouronic acid sodium salt (CAS). The influence of ultrasound treatment on the cellulose was investigated for obtaining CAS by regioselective oxidization using 4-acetamide-TEMPO and NaClO with NaClO2 as a primary oxidant in an aqueous buffer at pH 6.0. The yield, carboxylate content and polymerization degree (DP) of CAS were measured as a function of ultrasonic power, agitating time and cellulose consistency by an orthogonal test. The ultrasound-treated conditions were further improved by discussion of ultrasonic power, the most important factor influencing the yield and DP. An optimized CAS yield of 72.9% with DP value (DPv) of 212 was found when the ultrasonic strength is 550 W, agitating time is 3 h and cellulose consistency is 2.0%. The oxidation reactivity of cellulose was improved by ultrasonic irradiation, whereas no significant changes in crystallinity of cellulose were measured after ultrasonic treatment. Moreover, the ultrasound treatment has a greater effect on yielding CAS from parenchyma cellulose than from bagasse fibrous' one. The CAS was further characterized by Fourier transform infrared spectroscopy (FT-IR) and Scanning electron microscopy (SEM).
Canine distemper virus (CDV) infects wild and domestic Canidae worldwide. The hemag- glutinin (H) gene has the highest genetic variation in the genome of this virus. Thus, the H gene is commonly used for lineage identification and genetic analyses. In order to study the genetic characteristics and pathogenicity of CDV strains prevalent in China, 132 samples were collected from domestic dogs with suspected CDV infection, 58 samples were confirmed to be positive, and the H gene was successfully amplified from 15 samples. The epidemic strain was identified as type Asia-1 and the novel mutations, A51T, V58I, R179K and D262N, were detected in this strain. Isolated strains, BJ16B53, BJ16B14, and BJ17B8, were used for an animal infection experiment in raccoon dogs. BJ16B53 and BJ16B14 were found to cause clinical symptoms, death, and exten- sive lesions in various organs. These results are expected to facilitate the development of effective strategies to monitor and control CDV infection in China.