Synthetic refrigerants are being phased out gradually in accordance with international environmental protection protocols because of global warming and ozone layer depletion. Adopting R290/R600 refrigerant, an environmentally friendly refrigerant, to replace R134a, a high global warming potential refrigerant, provides one of the solutions. In this study, exergy analysis of R134a and TiO₂ suspended with lubricant and R290/R600 with a composition of 60% R290 and 40% R600 (60:40) was investigated in vapour compression system (VCRS) using R290/ R600 in TiO₂ nanomixture lubricant and compared with R134a and R290/ R600 in pure lubricant. At the inlets and outlets, the main components of the VCRS are connected to temperature and pressure sensors to measure the inlet and outlet temperatures and pressures. The results obtained were used to analyses the exergy losses at various VCRS components (compressor, condenser, evaporator, expansion valve) were investigated to determine the refrigerator’s total exergy destruction (E·x_dest.Total) and efficiency (η_ex). The E·x_dest.Total of R290/R600 in pure lubricant and R290/R600 TiO₂ nanomixture lubricant was reduced by 26.9% and 42.3%, respectively, and system η_ex increased by 27.7% and 38.9% respectively when compared to R134a in the system. Hence, TiO₂ suspended with R290/R600 is potential a substitute for R134a.