In this paper we present the first identification of the Tomato clack ring virus isolated from zucchini with mosaic and deformation of leaves in Poland. Immunosorbent electron microscopy, ELISA test and IC-RT-PCR confirmed the identification of TBRV. RNA extracted from purified virus (size about 7.4 kb and 4.6 kb) was characteristic to this virus.

From the naturally infected cucumber plane spherical virus was isolated that mainly on basis of its serological properties has been identified as Tomato black ring virus (TBRV). Using antiserum against TBRV-ED for the specific crapping of virus followed by PCR test (immunocapture-RT-PCR) allowed co distinguish TBRV from related viruses, especially Beet ringspot virus (BRSV). Presence of as many as rwo satellite RNAs should be found as a unique feature of the cucumber isolace.

This work presents some properties of Sunn-hemp mosaic tobamovirus (SHMV) orginally isolated from bean plants. Virus infected host range and induced symptoms that were typical for SHMV Following plant species distinguished SHMV from tobacco mosaic tobamovirus (TMVJ: Phaseolus vulgaris, Pisum sativum, Lupinus albus and Lycopersicon esculentum. In immunobloning the serum against SHMV did not react with TMV and Tomato mosaic tobamovirus (ToMV). The electrophoretical patterns of whole virions and capsid proteins were characteristic for SHMV and different from that of TMV and ToM.