Bacteria from the Simkaniaceae family are intracellular parasites belonging to the Chlamydiales order, detected in surface waters, drinking water, chlorine water, and in wastewater. Its main representative, Simkania negevensis, is pathogenic to humans and animals, especially fishes, as it principally causes respiratory tract diseases. Bacteria from this family are also capable of surviving and existing in free-living amoebas, omnipresent in the natural environment, which makes them an additional risk for human and animal health. The aim of the present study was to search for representatives of this family in freshwaters from the Odra River and two municipal lakes (Rusałka and Goplana). Out of 100 water samples analysed, the sequence of bacteria of Simkaniaceae family was found just in 1 percent, because phylogenetic analysis revealed that the obtained OdraWCh30 sequence shows 93% similarity to Simkania negevensis strain Z as well as 87% similarity to Candidatus Syngnamydia salmonis isolate Ho-2008 and Candidatus Syngnamydia salmonis isolate VS10102006 and 84-85% similarity to endosymbiont of Xenoturbella westbladi, Simkaniaceae bacterium clone SM081012-5s and Candidatus Syngnamydia venezia strain Pi3-2. This is the first case of detecting sequence of bacteria of Simkaniaceae family in the aquatic environment in Poland.
The European rabbit (Oryctolagus cuniculus) is a good model in biomedicine used in research on several human diseases. The reference values of B and T cells and their subpopu- lations are very important to understand how the adaptive immune system is responding to infectious agents. The aim of this study was to determine values of B and T cells and their subpopulations in Polish mixed-breed rabbits, considering seasons of the year and sex. The study was performed on 200 Polish mixed-breed rabbits and the percentage of B and T lymphocytes was measured cytometrically using mouse anti-rabbit antibodies. The study revealed that the season of the year and sex of the animals affected the percentage of B- and T-cells and their subpopulations in peripheral blood. Statistically significant values of CD19+ B-cells in spring and autumn, of T CD5+ cells in spring and winter, of T CD4+ in spring, summer, autumn and winter, of T CD8+ in winter and of T CD25+ in spring were noted. Generally the highest values were found mainly in warm part of the year, while the lowest in colder months. A statistical significance was also observed between males and females – changes were found in T CD4+ and T CD25+ lymphocytes in spring, T CD8+ cells in winter and higher percentage was generally obtained in females than in males. The only exception was the T CD5+ subpopulation in which no differences were observed between the sexes and throughout the year. This is the first paper on adaptive immune system cell values in the European rabbit of domestic breeds.
The difficulty of innovation risk assessment makes it necessary to use a multi-criteria analysis.
Innovative projects are related to unstructured problems and the uncertainty, therefore,
the use of fuzzy logic in the innovation risk assessment is analyzed. This paper proposes
a method of determining the weights of criteria in order to innovation risk assessment. The
weights are determined by 5 general criteria and 14 detailed criteria of innovation risk assessment.
The proposed method is an extension of the fuzzy AHP method. The extension
consists in taking into consideration the group decision-making approach with experts’ psychological
conditions. The groups of experts have been chosen based on an elaborated form.
The form makes it possible to characterize the persons within the scope of different psychological
conditions. The proposed method provides objective and rational decision-making.
The paper presents also a comparison of results with the fuzzy AHP method without the
group decision making. The weights obtained by the proposed method are more diversified
and bring out the most important criteria.
The paper is a continuation of the publication under the title “Acoustic diagnostics applications in the study of technical condition of combustion engine” and concerns the detailed description of decision support system for identifying technical condition (type of failure) of specified combustion engine. The input data were measured sound pressure levels of specific faults in comparison to the noise generated by undamaged motor. In the article, the whole procedure of decision method based on game graphs is described, as well as the interface of the program for direct usage.