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Application of prefabricated concrete in residential buildings and its safety management

L. Jiao X.D. Li
Archives of Civil Engineering | 2018 | Vol. 64 | No 2 | 21-35
Keywords prefabricated concrete residential building safety management
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Abstract

With the development of the society in recent years, there are more and more housing construction areas. The traditional concrete has not been able to satisfy the demand of housing construction. In this study, prefabricated concrete was applied in the design of assembling style houses considering their characteristics, and its economic, environmental and social benefits were analyzed combining risk matrix evaluation method and management strategy. It was found that the use of prefabricated concrete as a building material could effectively shorten the construction period, reduce the construction cost and improve the construction safety, playing a role of energy saving and environmental protection. Therefore it was concluded that prefabricated concrete can improve the efficiency of construction, reduce environmental pollution and save energy. This work provides a reference for the application of prefabricated concrete in residential buildings and its safety management.

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Authors and Affiliations

L. Jiao
X.D. Li

Research on the Influence of Excavation and Loading on Z-Direction Displacement in Surrounding Soil Mass

X. Li W. Zhou L. Han W.J. Xi Garmondyu E. Crusoe
Archives of Mining Sciences | 2014 | No 4 | DOI: 10.2478/amsc-2014-0066
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Authors and Affiliations

X. Li
W. Zhou
L. Han
W.J. Xi
Garmondyu E. Crusoe

Molecular cloning, recombinant expression, and purification of osteocalcin in sika deer (Cervus nippon) antler

X. Li M. Liu X. Bai Y. Li Y. Zhao S. Wang J. Wang
Polish Journal of Veterinary Sciences | 2019 | vol.22 | No 1 | 143-150 | DOI: 10.24425/pjvs.2018.125613
Keywords Cervus nippon osteocalcin molecular cloning expression purification
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Abstract

Osteocalcin is a major non-collagenous component of the bone extracellular matrix and is considered to be an indicative factor of osteoblast differentiation. In the present study, we detected osteocalcin expression in different antler areas and growth phases by immunohisto- chemistry. Osteocalcin was highly expressed in all areas during the mineralization period and in mesenchymal cell and chondrocyte areas during the rapid growth period. The nucleotide sequence of the osteocalcin gene in sika deer antler was determined. The open reading frame was 303 bp encoding a protein of 100 amino acids. The estimated molecular mass of osteocalcin was 10.38 kDa and the theoretical isoelectric point was 5.37. The osteocalcin gene with a 6× His-tag at the C-terminus was cloned into the pGEX-4T1 vector and expressed in Escherichia coli under optimal conditions. The recombinant soluble protein fused with GST was purified with Ni-NTA resin. The purified osteocalcin protein exhibited a significant increase in HA adhesion and promoted antler chondrocyte proliferation. Osteocalcin is an important factor in regulating the rapid growth and differentiation of deer antlers.

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Authors and Affiliations

X. Li
M. Liu
X. Bai
Y. Li
Y. Zhao
S. Wang
J. Wang

Expression and self-assembly of virus-like particles from porcine parvovirus and its application in antibody detection

Y. Li Q. Wang W. Yue X. Li Y. Chen Y. Gao
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 611-613 | DOI: 10.24425/pjvs.2023.148280
Keywords porcine parvovirus virus-like particles indirect ELISA
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Abstract

Porcine parvovirus (PPV) is a major causative agent in reproductive pig disease. The swine industry faces a significant economic and epizootic threat; thus, finding a reliable, quick, and practical way to detect it is essential. In this investigation, recombinant PPV VP2 protein was expressed in the Escherichia coli ( E. coli) expression systems. As shown by electron microscopy (TEM), Western blot, and hemagglutination (HA) assays, the recombinant VP2 protein was successfully assembled into virus-like particles (VLPs) after being expressed and purified. These VLPs had a structure that was similar to that of real PPV viruses and also exhibited HA activity. These VLPs induced high levels of PPV-specific antibody titers in mice after immunization, indicating that the VLPs may be beneficial as potential candidate antigens. VLPs were used as the coating antigens for the VLP ELISA, and the PPV VLPs-based ELISA displayed a high sensitivity (99%), specificity (93.0%) and agreement rate (98.3%) compared to HI assay, and the agreement rate of this ELISA was 97.5% compared to a commercial ELISA kit. Within a plate, the coefficient of variation (CV) was 10%, and between ELISA plates, the CV was 15%. According to a cross-reactivity assay, the technique was PPV-specific in contrast to other viral illness sera. The PPV VLP indirect-ELISA test for PPV detection in pigs with an inactivated vaccine showed that the PPV-positive rate varied among different sample sources from 88.2 to 89.6%. Our results indicate that this ELISA technique was quick, accurate, and repeatable and may be used for extensive serological research on PPV antibodies in pigs.
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Bibliography

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Authors and Affiliations

Y. Li
1
Q. Wang
2
W. Yue
1
X. Li
1
Y. Chen
1
Y. Gao
1
  1. Beijing Biomedicine Technology Center of JoFunHwa Biotechnology (Nanjing Co. Ltd.); No.25 Xiangrui Street Daxing District, Beijing 102600 China
  2. State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China

ABHD2 deficiency aggravates ovalbumin-induced airway remodeling through the PI3K/Akt pathway in an animal model of chronic asthma

L. Qiang X. Li Q. Li H. Bo Y. Liu M. Lv X. Chen H. Ju X. Sang Z. Li S. Jin
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 635-646 | DOI: 10.24425/pjvs.2023.148283
Keywords Abhd2 airway remodeling, inflammation PI3K/Akt signalling pathway
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Abstract

Airway remodeling is a major pathological characteristic of chronic obstructive pulmonary disease (COPD). This study aimed to investigate the effect of Abhd2 deficiency on ovalbumin (OVA)-induced airway remodeling and inflammation in vivo. Abhd2-deficient mice were used to establish an OVA-induced asthma model. Lung tissues were analyzed using hematoxylin and eosin (HE) staining, Masson staining, immunohistochemistry, quantitative reverse transcription- polymerase chain reaction (qRT-PCR), and western blotting were used to determine the role of Abhd2 in the regulation of OVA-induced airway remodeling and inflammation. Our findings revealed that the RNA expression of inflammatory factors, including IL-1β, IL-6, IL-4, and IL-13, was significantly increased in OVA-induced Abhd2 Gt/Gt asthmatic mice. The expression of IFN-γ was decreased significantly in OVA-induced Abhd2 Gt/Gt asthmatic mice. The protein expression of airway remodeling factors, including α-SMA, type I collagen, and Ki67, was also increased in OVA-induced Abhd2 Gt/Gt asthmatic mice compared to that in OVA-induced wild-type (WT) mice. Additionally, Abhd2 deficiency promoted the expression of p-Akt in tissues of the asthma model. These results suggest that Abhd2 deficiency exacerbates airway remodeling and inflammation through the PI3K/Akt pathway in chronic asthma.
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Authors and Affiliations

L. Qiang
1
X. Li
1
Q. Li
2
H. Bo
3
Y. Liu
1
M. Lv
1
X. Chen
1
H. Ju
1
X. Sang
1
Z. Li
4
S. Jin
1
  1. Department of Respiratory Medicine, Fourth Affiliated Hospital, Harbin Medical University, 37# Yiyuan Street, Harbin 150001, Heilongjiang, China
  2. Department of pulmonary diseases, Heilongjiang Academy of Traditional Chinese Medicine, 33# Xidazhi Street, Harbin 150036, Heilongjiang, China
  3. Department of Intensive Care Unit, Fourth Affiliated Hospital, Harbin Medical University, 37# Yiyuan Street, Harbin 150001, Heilongjiang, China
  4. University of Tokyo, 3-8-1# Bunkyo ku, Tokyo 1130033, Tokyo, Japan

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