In vitro test of antagonistic activity of culture filtrates from Trichoderma harzianum Rifai and Trichoderma pseudo-koningii Rifai strains against post-harvest pathogens of some fruits were investigated. The undiluted culture filtrates of the two Trichoderma species completely inhibited germination of conidia/spores of all the rot pathogens, but 50% dilution showed varying degree of inhibition of spore germination. T. pseudo-koningii culture filtrate had a rather moderate to strong inhibitory effect on mycelia of the pathogenic fungi. The highest per cent inhibition of 45.6% of mycelial growth was recorded for Aspergillus niger Tiegh.