Abstract
Vibrio species are common inhabitants of aquatic environments and have been described in connection with fish and human diseases.
Six Vibrio species were isolated from diseased freshwater and ornamental fish in Poland. The strains were identified based on morphological and biochemical characteristics and confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) as V. albensis (n=3) from Gymnocephalus cernua, Sander lucioperca, Paracheirodon innesi, and Xiphophorus hellerii; V. mimicus (n=1) from Xiphophorus maculatus; and V. vulnificus (n=1) from Nematobrycon palmeri. This is the first time that Vibrio species have been isolated and described from ornamental fish in Poland. The isolates were resistant to ampicillin (83.3%), gentamicin (16.6%), ciprofloxacin (16.6%), sulfamethoxazole-trimethoprim (16.6%), and chloramphenicol (16.6%). The multiple antibiotic resistance (MAR) index was 0.00-0.08 for V. albensis, 0.17 for V. mimicus, and 0.33 for V. vulnificus.
Our study confirmed the presence of potentially pathogenic Vibrio species in freshwater and ornamental fish. Therefore, further monitoring of the presence of Vibrio species, mainly in ornamental fish, is necessary.
Go to article
Bibliography
1. Aksak-Wąs BJ, Ripa A, Szakoła P, Horbacka K, Niścigorska-Olsen J, Witak-Jędra M, Zając-Marczewska M, Karasińska-Cieślak M, Kot J, Parczewski M (2021) Septic shock induced by Vibrio vulnificus in Northen Poland, a case report. Infect Drug Resist 14: 5027-5033.
2. Al-Dulaimi MM, Mutalib SA, Ghani MA, Zaini NA, Ariffin AA (2019) Multiple antibiotic resistance (MAR), plasmid profiles, and DNA polymorphisms among Vibrio vulnificus isolates. Antibiotics (Basel) 8: 68.
3. Araj GF, Taleb R, El Beayni NK, Goksu E (2019) Vibrio albensis: An unusual urinary tract infection in a healthy male. J Infect Public Health 12: 712-713.
4. Austin B, Austin DA (2007) Vibrios. In: Austin B, Austin DA (eds) Bacterial fish pathogens. Diseases of farmed and wild fish. Praxis Publishing Ltd, Chichester, UK, pp 499-601.
5. Baker-Austin C, McArthur JV, Lindell AH, Wright MS, Tuckfield RC, Gooch J, Warner L, Oliver J, Stepanauskas R (2009) Multi-site analysis reveals widespread antibiotic resistance in the marine pathogen Vibrio vulnificus. Microb Ecol 57: 151-159.
6. Baker-Austin C, Oliver JD, Alam M, Ali A, Waldor MK, Qadri F, Martinez-Urtaza J (2018) Vibrio spp. infections. Nat Rev Dis Primers 4: 8.
7. Bauer AW, Kirby WM, Sherris JC, Turck M (1966) Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45: 493-496.
8. Bisharat N, Agmon V, Finkelstein R, Raz R, Ben-Dror G, Lerner L, Soboh S, Colodner R, Cameron DN, Wykstra DL, Swerdlow DL, Farmer JJ 3rd (1999) Clinical, epidemiological, and microbiological features of Vibrio vulnificus biogroup 3 causing outbreaks of wound infection and bacteraemia in Israel. Israel Vibrio Study Group. Lancet 354: 1421-1424.
9. Cassini A, Högberg LD, Plachouras D, Quattrocchi A, Hoxha A, Simonsen GS, Colomb-Cotinat M, Kretzchmar ME, Devleesschauwer B, Cecchini M, Ouakrim DA, Oliveira TC, Struelens MJ, Suetens C, Monnet DL, Burden of AMR Collaborative Group (2019) At-tributable deaths and disability-adjusted life-years caused by infections with antibiotic-resistant bacteria in the EU and the European Eco-nomic Area in 2015: a population-level modelling analysis. Lancet Infect Dis 19: 5-66.
10. Cheng WC, Jan IS, Chen JM, Teng SH, Teng LJ, Sheng WH, Ko WC, Hsueh PR (2015) Evaluation of the Bruker biotyper ma-trix-assisted laser desorption ionization-time of flight mass spectrometry for identification of blood isolates of Vibrio species. J Clin Mi-crobiol 53: 1741-1744.
11. Clinical and Laboratory Standards Institute – CLSI (2015) Methods for antimicrobial dilution and disk susceptibility testing of infre-quently isolated or fastidious bacteria. 3rd ed. CLSI guideline M45. Wayne, PA.
12. Da Silva LV, Ossai S, Chigbu P, Parveen S (2021) Antimicrobial and genetic profiles of Vibrio vulnificus and Vibrio parahaemolyticus isolated from the Maryland Coastal Bays, United States. Front Microbiol 12: 676249.
13. Dieckmann R, Strauch E, Alter T (2010) Rapid identification and characterization of Vibrio species using whole-cell MALDI-TOF mass spectrometry. J Appl Microbiol 109: 199-211.
14. Dong HT, Nguyen VV, Le HD, Sangsuriya P, Jitrakorn S, Saksmerprome V, Senapin S, Rodkhum C (2015) Naturally concurrent in-fections of bacterial and viral pathogens in disease outbreaks in cultured Nile tilapia (Oreochromis niloticus) farms. Aquaculture 448: 427-435.
15. El-Deen AG, Elkamel AA (2015) Clinical and experimental study on vibriosis in ornamental fish. Assiut Vet Med J 61: 147-153.
16. Erler M, Wichels A, Heinemayer EA, Hauk G, Hippelein M, Reyes NT, Gerdts G (2015) VibrioBase: A MALDI-TOF MS database for fast identification of Vibrio spp. that are potentially pathogenic in humans. Syst Appl Microbiol 38: 16-25.
17. Esteve C, Alcaide E, Herraiz S, Canals R, Merino S, Tomás JM (2007) First description of nonmotile Vibrio vulnificus strains virulent for eels. FEMS Microbiol Lett 266: 90-97.
18. Geng Y, Liu D, Han S, Zhou Y, Wang KY, Huang XL, Chen DF, Peng X, Lai WM (2014) Outbreaks of vibriosis associated with Vib-rio mimicus in freshwater catfish in China. Aquaculture 433: 82-84.
19. Huzmi H, Ina-Salvany MY, Natrah FM, Syukri F, Karim M (2019) Strategies of controlling vibriosis in fish. Asian J Appl Sci 7: 513-521.
20. Kiani S, Naghavi NS, Nazari A (2016) Detection of Vibrio species isolated from ornamental guppy fish in Kashan, Isfahan, Iran fish culturing ponds. Biol J Microorg 4: 43-48.
21. Kiiyukia C, Nakajima A, Nakai T, Muroga K, Kawakami H, Hashimoto H (1992) Vibrio cholera non-O1 isolated from ayu fish (Pleco-glossus altivelis) in Japan. Appl Environ Microbiol 58: 3078-3082.
22. Kolada A (ed.) (2022) Wstępny raport ds. sytuacji na rzece Odrze. IOŚ-PIB. https://ios.edu.pl/wp-content/uploads/2022/10/
Wstepny-raport-zespolu-ds.-sytuacji-na-rzece-Odrze.pdf
23. Krumperman PH (1983) Multiple antibiotic resistance indexing Escherichia coli to identify high-risk sources of fecal contamination of foods. Appl Environ Microbiol 46: 165-170.
24. Kumarage PM, De Silva LADS, Heo G-J (2022) Aquatic environments: A potential source of antimicrobial-resistant Vibrio spp. J Appl Microbiol 133: 2267-2279.
25. Kurpas M, Michalska M, Zakrzewski A, Zorena K (2021) First report of the presence of Vibrio vulnificus in the Gulf of Gdansk. Int Ma-rit Health 72: 247-251.
26. Lupiani B, Baya AM, Magariños B, Romalde JL, Li T, Roberson BS, Hetrick FM, Toranzo AE (1993) Vibrio mimicus and Vibrio chol-erae non-01 isolated from wild and hatchery-reared fish. Gyobyo Kenkyu 28: 15-26.
27. Manchanayake T, Salleh A, Amal MN, Yasin IS, Zamri-Saad M (2023) Pathology and pathogenesis of Vibrio infection in fish: A re-view. Aquac Rep 28: 101459.
28. Manfrin A, Friso S, Perin R, Qualtieri K, Bovo G, Rodgers CJ (2001) Tropical fish importation from third countries: the potential risk of introducing human and aquatic animal pathogens. Risk analysis in aquatic animal health. In: Proceedings of an International Conference, OIE Headquarters, Paris, France. 8–10 February 2000, pp 167–169.
29. Noorlis A, Ghazali FM, Cheah YK, Tuan Zainazor TC, Wong WC, Tunung R, Pui CF, Nishibuchi M, Nakaguchi Y, Son R (2011) An-tibiotic resistance and biosafety of Vibrio cholera and Vibrio parahaemolyticus from freshwater fish at retail level. Int Food Res J 18: 1523-1530.
30. Osunla CA, Okoh AI (2017) Vibrio Pathogens: A public health concern in rural water resources in Sub-Saharan Africa. Int J Environ Res Public Health 14: 1188.
31. Rehulka J, Petras P, Marejkova M, Aldova E (2015) Vibrio choleae non-O1/non-O139 infection in fish in the Czech Republic. Vet Med - Czech 60: 16-22.
32. Senderovich Y, Izhaki I, Halpern M (2010) Fish as reservoirs and vectors of Vibrio cholerae. PLoS One 5: e8607
33. Shaw KS, Goldstein RE, He X, Jacobs JM, Crump BC, Sapkota AR (2014) Antimicrobial susceptibility of Vibrio vulnificus and Vibrio parahaemolyticus recovered from recreational and commercial areas of Chesapeake Bay and Maryland Coastal Bays. PLoS One 9: e89616.
34. Stypułkowska-Misiurewicz H, Pancer K, Roszkowiak A (2006) Two unrelated cases of septicaemia due to Vibrio cholerae non-O1, non-O139 in Poland, July and August 2006. Euro Surveill 11: 3088.
35. Stypułkowska-Misiurewicz H, Stasiak J, Janczyk M, Tomaszewska E, Pancer K (1995) Vibrio cholerae non-01 isolated in Poland from the Bug river. Przegl Epidemiol 49: 237-243.
36. Tison DL, Nishibuchi M, Greenwood JD, Seidler RJ (1982) Vibrio vulnificus biogroup 2: new biogroup pathogenic for eels. Appl En-viron Microbiol 44: 640-646.
37. Vandenberghe J, Thompson FL, Gomez-Gil B, Swings J (2003) Phenotypic diversity amongst Vibrio isolates from marine aquaculture systems. Aquaculture 219: 9-20.
38. Warner E, Oliver JD (2008) Population structures of two genotypes of Vibrio vulnificus in oysters (Crassostrea virginica) and seawater. Appl Environ Microbiol 74: 80-85.
39. Zago V, Zambon M, Civettini M, Zaltum O, Manfrin A (2017) Virulence-associated factors in Vibrio cholerae non-O1/non-O139 and V. mimicus strains isolated in ornamental fish species. J Fish Dis 40: 1857-1868.
40. Zhang X, Li YW, Mo ZQ, Luo XC, Sun HY, Liu P, Li AX, Zhou SM, Dan XM (2014) Outbreak of a novel disease associated with Vibrio mimicus infection in fresh water cultured yellow catfish, Pelteobagrus fulvidraco. Aquaculture 432: 119-124.
Go to article
en
pl
