The study took place between 2012 and 2014 in Falenty near Warsaw, Poland, as part of a long-term scientific experi-ment (first began in 1987) using the randomized block method. All blocks were irrigated until 2008. In 2009 each block was divided into two areas: irrigated and non-irrigated. The study involved four levels of inorganic nitrogen fertilizer and two levels of mixed inorganic and organic fertilizer in the form of fermented cattle urine. The soil in all experimental plots was characterized by low levels of zinc, ranging from 7.6 to 16.7 mg Zn∙kg–1 dry matter. Much lower Zn content in both soil layers of all irrigated plots was associated with increased yields on these plots, regardless of the level and form of ferti-lizer. The content of Zn in soil and sward in 2014 year was significantly lower compared in 2012. Inadequate levels of zinc for ruminant nutrition were observed in the sward from all plots (15.4–28.8 mg∙kg–1 dry matter). The higher content of zinc was found in sward harvested from the plot, which was not fertilized with phosphorus. The long-term inorganic and fer-mented urine fertilization resulted in very low zinc content in the soil and meadow sward.