The root-knot nematode Meloidogyne graminicola is an economically important pest in rice production. The identification of a nematode species is an important basis in nematode management to reduce yield losses by extracting nematode DNA as an early step in molecular identification. This study aimed to investigate the optimal extraction method and number of M. graminicola for nematode genomic analysis based on PCR (polymerase chain reaction) and Sanger sequencing. The DNA extraction methods used in this study were the CTAB, SDS, and commercial kit (GeneAidTM Tissue/Blood DNA Mini Kit). The results revealed that the three DNA extraction methods could be used to analyze the nematode genomics based on PCR and Sanger sequencing using one nematode, both in a second-stage juvenile and a female, equipped with the process of nematode destruction by freezing. This finding was shown by the amplification of all DNA templates with Mg-F3 and Mg-R2 primers through PCR with a size of 370 bp, while Sanger sequencing obtained 372 bp.

Tea mosquito bugs (TMB; Helopeltis bradyi, Hemiptera, Miridae) are a main pest in tea and cacao plantations worldwide. Pheromone-mediated mating disruption (MD) is a promising strategy to manage TMB populations. Experiments were conducted to evaluate the simultaneous effect of TMB female delayed mating (1, 3 and 5 days delayed mating) and pairing duration (1 day, 5 days, and entire lifespan) on several reproductive parameters. Results showed that female delayed mating obviously affected egg number, egg viability, and offspring number, but male delayed mating did not show such effects. Shortened pairing durations had a significant effect on egg viability and offspring number but not on egg number. Increased delayed mating and pairing duration negatively affected adult longevity. In general, we noted that TMB reproductive performance is vulnerable to delayed mating and shortened pairing durations, thus providing essential guidance for the implementation of MD strategies of TMB in the field.