The carriage of pathogenic Leptospira was investigated by PCR in 51 wild carnivores, 20 domestic dogs with outdoor access, and 27 free-roaming domestic cats sampled in periurban Barcelona (NE Spain). Overall prevalence was 7.7%, with DNA confirmed in 3/30 common genets (Genetta genetta) (serovars Icterohaemorraghiae and Sejröe), 1/9 red foxes (Vulpes vulpes) (Canicola) and 2/27 cats (Icterohaemorraghiae). Though most of the dogs were vaccinated against Leptospira, DNA of the serovar Canicola was detected in the urine of 25% of the vaccinated animals, and the serovar Icterohaemorraghiae in one non-vaccinated dog.