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Number of results: 18
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Abstract

In recent years, infections are more often caused by pathogens with high multi-drug resistance, classified as the “ESKAPE” microorganisms. Therefore, investigation of these pathogens, e.g., Klebsiella pneumoniae, often requires biomass production for treatment testing such as antibiotics or bacteriophages. Moreover, K. pneumoniae can be successfully applied as a biocatalyst for other industrial applications, increasing the need for this bacteria biomass. In the current study, we proposed a novel magnetically assisted bioreactor for the cultivation of K. pneumoniae cells in the presence of an external alternating magnetic field (AMF). High efficiency of the production requires optimal bacteria growth conditions, e.g., temperature and field frequency. Therefore, we performed an optimization procedure using a central composite design for these two parameters in a wide range. As an objective function, we utilized a novel, previously described growth factor that considers both biomass and bacteria growth kinetics. Thus, based on the response surface, we could specify the optimal growth conditions. Moreover, we analysed the impact of the AMF on bacteria proliferation, which indicated positive field frequency windows, where the highest stimulatory effect of AMF on bacteria proliferation occurred. Obtained results proved that the magnetically assisted bioreactor could be successfully employed for K. pneumoniae cultivation.
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Authors and Affiliations

Maciej Konopacki
1 2
ORCID: ORCID
Adrian Augustyniak
1 3
ORCID: ORCID
Bartłomiej Grygorcewicz
1 2
ORCID: ORCID
Barbara Dołęgowska
2
ORCID: ORCID
Marian Kordas
1
ORCID: ORCID
Rafał Rakoczy
1
ORCID: ORCID

  1. West Pomeranian University of Technology in Szczecin, Faculty of Chemical Technology and Engineering, Department of Chemical and Process Engineering, al. Piastów 42, 71-065 Szczecin, Poland
  2. Pomeranian Medical University in Szczecin, Chair of Microbiology, Immunology and Laboratory Medicine, Department of Laboratory Medicine, al. Powstanców Wielkopolskich 72, 70-111 Szczecin, Poland
  3. Technische Universität Berlin, Building Materials and Construction Chemistry, Gustav-Meyer Allee 25,13355 Berlin, Germany
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Abstract

The electrostatic impulse method is an established method for producing microbeads or capsules. Such particles have found application in biomedical engineering and biotechnology. The geometric properties of the droplets – constituting precursors of microbeads and capsules – can be precisely controlled by adjusting the geometry of the nozzle system, the physical properties and the flow rate of the fluids involved, as well as the parameters of the electrostatic impulse. In this work, a method of mathematical modeling of the droplet generation process using the electrostatic impulse method in a single nozzle system is presented. The developed mathematical model is an extension of the standard Volume of Fluid (VOF) model by addition of the effect of the electric field on the fluid flow. The model was implemented into the OpenFOAM toolkit for computational fluid dynamics (CFD). The performed CFD simulation results showed good agreement with experimental data. As a result, the influence of all process parameters on the droplet generation process was studied. The most significant change in droplet generation was caused by changing the electrostatic impulse strength. The presented modeling method can be used for optimization of process design and for studying the mechanisms of droplet generation. It can be extended to describe multi nozzle systems used for one-step microcapsule production.
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Authors and Affiliations

Piotr Cendrowski
1
ORCID: ORCID
Katarzyna Kramek-Romanowska
1
ORCID: ORCID
Dorota Lewińska
2
ORCID: ORCID
Marcin Grzeczkowicz
2
ORCID: ORCID
Paulina Korycka
3
ORCID: ORCID
Jan Krzysztoforski
1
ORCID: ORCID

  1. Warsaw University of Technology, Faculty of Chemical and Process Engineering, ul. Ludwika Warynskiego 1, Warsaw, Poland
  2. Nalecz Institute of Biocybernetics and Biomedical Engineering Polish Academy of Sciences, ul. Ks. Trojdena 4, Warsaw, Poland
  3. Foundation of Research and Science Development, Rydygiera 8, 01-793 Warsaw, Poland
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Abstract

Chitosan (CS) is a polysaccharide readily used in tissue engineering due to its properties: similarity to the glycosaminoglycans present in the body, biocompatibility, non-toxicity, antibacterial character and owing to the fact that its degradation that may occur under the influence of human enzymes generates non-toxic products. Applications in tissue engineering include using CS to produce artificial scaffolds for bone regeneration that provide an attachment site for cells during regeneration processes. Chitosan can be used to prepare scaffolds exclusively from this polysaccharide, composites or polyelectrolyte complexes. A popular solution for improving the surface properties and, as a result enhancing cellbiomaterial interactions, is to coat the scaffold with layers of chitosan. The article focuses on a polysaccharide of natural origin – chitosan (CS) and its application in scaffolds in tissue engineering. The last part of the review focuses on bone tissue and interactions between cells and chitosan after implantation of a scaffold and how chitosan’s structure affects bone cell adhesion and life processes.
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Authors and Affiliations

Anna Kołakowska
1
ORCID: ORCID
Agnieszka Gadomska-Gajadhur
1
ORCID: ORCID
Paweł Ruśkowski
1
ORCID: ORCID

  1. Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw, Poland
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Abstract

Mikhail Zoshchenko is one of the most famous Russian humourists and writers of the first half of 20th century. His texts, and in particular the short stories written in 1920s–1930s, are true masterpieces and had an extraordinary success in Russia, echoed in an immediate reception abroad. The aim of this contribution is to analyse some samples from the ten Italian translations of one of his most iconic short stories, Aristokratka (1924). Particular attention will be given to the way the translators dealt with the different aspects of the language used by Zoshchenko, a perfect specimen of a modern realisation of the skaz.
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Authors and Affiliations

Noemi Albanese
1
ORCID: ORCID

  1. Università degli Studi di Roma Tor Vergata
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Abstract

This paper offers an analysis of specialised medical discourse about COVID-19 in scientific articles in English, published in Polish professional medical journals. Special attention is paid to the textual representation of phenomena such as coronavirus and other pandemic-related concepts/aspects, as well the agency of doctors and patients. Methodologically, the paper blends the principles of qualitative discourse analysis with insights obtained from a quantitative exploration of the texts with the help of SketchEngine®.
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Authors and Affiliations

Magdalena Zabielska
1
ORCID: ORCID

  1. Adam Mickiewicz University, Poznań
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Abstract

The aim of this article is to survey the Polish translation of The Queen’s Corgie (2019), the animated movie, in terms of the dubbese employed therein. Apart from the standard features such as slang, jargon, rhyming, and intertextual references, we focus on the use inventive language pertaining to the domain of dogs, which could be accounted for by means of the analysability/compositionality parameter as proposed by Langacker in lockstep with the blending theory as developed by Fauconnier and Turner.
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Authors and Affiliations

Konrad Żyśko
1
ORCID: ORCID

  1. Maria Curie-Skłodowska University, Lublin
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Abstract

Adapting a literary text for a TV series within the same culture involves a plethora of interpretive, semiotic, and hermeneutic relationships. Therefore, adapting and (re)translating a TV series for a foreign audience and culture is even more challenging. The code-switching and code-mixing used in Gomorra- The Series represent a great challenge for the translator. Thus, the aim of the paper is to focus on the subtitling strategies used to translate culture-bound language in the English version of Gomorra-The Series, an extremely successful Italian TV series.
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Authors and Affiliations

Giulia Magazzù
1
ORCID: ORCID

  1. Università degli Studi 'G. d'Annunzio' Chieti - Pescara
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Abstract

This paper examines the first English translation of Colmenero’s Curioso Tratado de la Naturaleza y Calidad del Chocolate (1631). The analysis of the English version, Chocolate, or an Indian Drinke, by James Wadsworth is carried out from a qualitative perspective, paying special attention to the translated strategies deployed. The research reveals that Wadsworth will chiefly use procedures such as the preservation of indigenous words and extension, as well as other techniques to a lesser extent.
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Authors and Affiliations

Isabel De La Cruz-Cabanillas
1
ORCID: ORCID

  1. University of Alcalá
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Abstract

The aim of this paper is to examine the phenomenon of infixation in the Spanish language. This study has been divided into five parts, with the exception of the introduction. The first one presents ways of classifying various affixes according to their position within words. In the next two parts, two different types of infixation in Spanish are presented. The first of them concerns diminutive forms and has an expressive function in them. The second one appears in vesreisms (Sp. vesreísmos), where it indicates the grammatical gender of nouns and adjectives. The first infixes occur in all varieties of the Spanish language, while the latter are dialectal. In the fourth part, an attempt is made to propose further arguments in favor of recognizing the existence of infixed forms in Spanish. In the last part of the article, some conclusions are drawn on the basis of the examples and observations presented in the previous parts of the study.
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Authors and Affiliations

Piotr Sorbet
1
ORCID: ORCID

  1. Maria Curie-Skłodowska University, Lublin
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Abstract

Apart from verbs, languages possess morphemes which are related to time without this connection being obvious to the speakers or felt by them. Such is the case of the articles, certain prepositions, conjunctions and particles. In the present article, I analyse particularly the indefinite and definite articles. These create a sequence that actualises a noun and then reactualises it, giving it thereby a past. Thus, I describe actualisation with the help of articles, but I also take up to discussion sequential and contextual actualisation. The examples I quote come mainly from French but, since actualisation is a general linguistic phenomenon, some of them represent languages which are structurally different from each other. The temporality rendered by the grammatical morphemes can express either past time or future time.
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Authors and Affiliations

Hans Lagerqvist
1

  1. Universite d’Uppsala, Sweden
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Abstract

In this work Response Surface Methodology and Central Composite Rotatable Design were applied to find high-energy mixing process parameters enabling flow properties of highly cohesive Disulfiram powder to be improved. Experiments were conducted in a planetary ball mill. The response functions were created for an angle of repose and compressibility index as measures of powder flowability. To accomplish an optimisation procedure of mixing process parameters according to a desirability function approach, the results obtained earlier for potato starch, as another cohesive coarse powder, were also employed. Coupling these results with those achieved in a previous work, it was possible to develop some guidelines of practical importance allowing mixing conditions to be predicted towards flow improvement of fine and coarse powders.
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Authors and Affiliations

Karolina M. Leś
1
ORCID: ORCID
Ireneusz Opaliński
1
ORCID: ORCID

  1. Department of Chemical and Process Engineering, Rzeszow University of Technology, al. Powstanców Warszawy 6, 35-959 Rzeszow, Poland
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Abstract

In this article, we present the results of the first application of 2–benzoylpyridine (2–BP) as a carrier in adsorptive polymeric materials dedicated for the removal of Ag(I) and Cu(II) ions from model acidic solutions. In the first stage of the research, the classical solvent extraction, in which 2–BP was used as an extractant, allowed to determine the proper conditions for conducting adsorptive processes. The stability constants of 2–BP complexes with analyzed metal ions were determined using the spectrophotometric method. The electrospray ionization (ESI) high-resolution mass spectrometry (HRMS) method was applied for the confirmation of the ability of 2–BP molecules to form complexes with Cu 2+¸ metal ions in a solution and to determine the elemental composition of generated complexes (to identify the ratio of the number of metal ions to the number of molecules of 2–BP). The obtained results indicate that both the adsorptive processes and solvent extraction strongly depend on the properties of metal ions and that the use of 2–BP as a carrier/extractant allows for efficient removal of silver(I) ions and much less effective removal of copper(II) ions. The utilization of adsorptive polymeric materials is in line with the contemporary research trends that focus on eco-friendly and cost-effective methods.
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Authors and Affiliations

Małgorzata A. Kaczorowska
1
ORCID: ORCID
Daria Bożejewicz
1
ORCID: ORCID
Katarzyna Witt
1
ORCID: ORCID
Włodzimierz Urbaniak
2
ORCID: ORCID

  1. Bydgoszcz University of Science and Technology, Faculty of Chemical Technology and Engineering, Seminaryjna 3, 85-326 Bydgoszcz, Poland
  2. Adam Mickiewicz University, Poznan, Faculty of Chemistry, Uniwersytetu Poznanskiego 8, 61-712 Poznan, Poland
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Abstract

The following work gives the details of the modelling, simulation, and testing of a small portable gravitational water vortex (GWV) based power plant. The gravitation water vortex is an ideal source of renewable energy for rural areas that have a small body of flowing water. For this purpose, we have selected a small size for the vortex chamber that enables it to form a vortex with limited amounts of water. The paper gives the details of the simulation of the GWV in COMSOL FEA software and the parameters that were chosen for optimization. These parameters were the height of the vortex chamber, the number of blades, the length of the blades, and the tilt angle of the blades. These parameters were systematically varied step by step, to observe their effect on the speed of the rotor. The results of the parametric sweep that was performed on all the parameters are also presented. Based on the simulation results an optimal set of parameters was chosen for the physical implementation of the GWV. The paper also goes into the details of the construction of the physical GWV, the experimental setup that was devised for the testing and verification of the simulation results.
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Authors and Affiliations

Vinayakumar B.
1
ORCID: ORCID
Rahul Antonyo
1
ORCID: ORCID
Binson V.A.
1
ORCID: ORCID
Youhan Sunnyo
1
ORCID: ORCID

  1. Saintgits College of Engineering, Pathamuttom P.O Kottayam, Kerala, India Pincode: 686532
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Abstract

Developing an effective and safe cancer therapy could significantly reduce the number of deaths and improve the quality of life of treated patients. Nowadays medicine has developed a wide range of anticancer chemotherapeutics but at the same time there is a lack of effective drug delivery methods. Therefore, the development of the targeted drug delivery system which will selectively release drug into the cancer cells is a key challenge of modern medicine.
The main aim of the presented research was to investigate the targeting effect of a drug delivery system based on the controlled release of dextran nanoparticles containing the anticancer drug – doxorubicin from the alginate microspheres coated with chitosan multilayers.
During the research the physicochemical properties of the alginate microspheres and its stability in the physiological environment were investigated. Moreover, the kinetics of the nanoparticles with doxorubicin release from the alginate microspheres covered with chitosan multilayers was characterized, depending on the thickness of the chitosan layer. Further, the cytotoxicity study of the alginate microspheres covered with chitosan multilayer and containing nanoparticles was performed to determine the therapeutic effect of the released nanoparticles with doxorubicin on the HeLa cells during the in vitro cell culture.
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Authors and Affiliations

Aleksandra Kulikowska-Darłak
1
ORCID: ORCID
Agata Stefanek
1
ORCID: ORCID
Iga Wasiak-Wojasińska
1
ORCID: ORCID
Paulina Wiechecka-Ożdżyńska
1
ORCID: ORCID
Tomasz Ciach
1
ORCID: ORCID

  1. Faculty of Chemical and Process Engineering, Warsaw University of Technology, Warynskiego 1 Street, 00-645 Warsaw, Poland
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Abstract

Halloysite is a filler which may be used to produce composites with thermoplastic polymer matrix. This work summarized the results of investigations of processing, structural, mechanical, and thermal properties of the composites with poly(vinyl chloride) (PVC) matrix and raw halloysite (HA) as well as its calcined product (KHA). The effectiveness of calcination was confirmed with X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy, and nitrogen adsorption method. The PVC composites with HA as well as KHA were processed in the molten state in the Brabender mixer chamber. The reduction of gelation time and simultaneous increase in maximum torque with filler content were found based on the results of plastographometric analysis. SEM images of PVC/halloysite composites showed a homogeneous distribution of the filler in the polymer matrix. The introduction of halloysite leads to a slight increase in Young’s modulus and tensile strength compared to neat PVC, where the increase of both parameters is greater when KHA is used. The incorporation of 1% KHA led to an increase in impact strength, an effect which may be attributed to toughening of the polymer. A slight improvement of the Vicat softening temperature of 2.7°C for PVC/HA and heat deflection temperature of 2.4°C for PVC/KHA was also ascertained for PVC modified with 10 wt% of filler.
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Authors and Affiliations

Martina Wieczorek
1
ORCID: ORCID
Jolanta Tomaszewska
1
ORCID: ORCID
Tomasz Bajda
2
ORCID: ORCID
Jacek Długosz
3
ORCID: ORCID

  1. Bydgoszcz University of Science and Technology, Faculty of Chemical Technology and Engineering, Al. Prof. S. Kaliskiego 7, 85-796 Bydgoszcz, Poland
  2. AGH University of Science and Technology, Faculty of Geology, Geophysics and Environmental Protection, Al. A. Mickiewicza 30, 30-059, Kraków, Poland
  3. Bydgoszcz University of Science and Technology, Faculty of Agriculture and Biotechnology, Al. Prof. S. Kaliskiego 7, 85-796 Bydgoszcz, Poland
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Abstract

This paper presents an analysis of the impact of inertial forces of the electrolyte flow in an interelectrode gap on the effects of ECM process of curvilinear rotary surfaces. Considering a laminar flow in the interelectrode gap, the equations of the flow of the mixture of electrolyte and hydrogen in the curvilinear orthogonal coordinate system have been defined. Two classes of equations of motion have been formulated, which differ in the estimates referred to the components of velocity and pressure, and which were analytically solved using the method of perturbation.
Using the machined surface shape evolution equation, the energy equation, and the analytical solutions for velocity and pressure, the ECM-characteristic distributions have been determined: of mean velocity, pressure, mean temperature, current density, gas phase concentration, the gap height after the set machining time for the case when there is no influence of inertial forces, the effect of centrifugal forces and, at the same time, centrifugal and longitudinal inertial forces.
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Authors and Affiliations

Jerzy Sawicki
1
ORCID: ORCID
Tomasz Paczkowski
2
ORCID: ORCID
Jarosław Zdrojewski
3
ORCID: ORCID

  1. Department of Mechanics and Computer Methods, Bydgoszcz University of Science and Technology, Bydgoszcz, Poland
  2. Department of Manufacturing Techniques, Bydgoszcz University of Science and Technology, Bydgoszcz, Poland
  3. Department of Digital Technology, Bydgoszcz University of Science and Technology, Bydgoszcz, Poland

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