Abstrakt
Self-biting disease occurs in most farmed fur animals in the world. The mechanism and rapid
detection method of this disease has not been reported. We applied bulked sergeant analysis
(BSA) in combination with RAPD method to analyze a molecular genetic marker linked with
self-biting trait in mink group. The molecular marker was converted into SCAR and loop-mediated
isothermal amplification (LAMP) marker for rapid detection of this disease. A single RAPD
marker A10 amplified a specific band of 1000bp in self-biting minks. The sequences of the bands
exhibited 73% similarity to the Canis Brucella. SCAR and LAMP marker were designed for the
specific fragment of RAPD marker A10 and validated in 30 self-biting minks and 30 healthy
minks. c2 test showed difference (p<0.05) with SCAR and significant difference (p<0.01) with
LAMP in the detection rate between the two groups, but LAMP method was more accurate than
SCAR method. This indicated that LAMP can be used as a positive marker to detect self-biting
disease in minks.
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