Abstract
Effect of single nucleotide polymorphism (SNP) in splicing site of the LPAR1 (lysophosphatidic
acid receptor 1) gene on selected quality traits was investigated in frozen-thawed semen
of Holstein-Friesian bulls. Splicing mutation A/G in the LPAR1 gene (rs43581860) was identified
in 120 Holstein-Friesian bulls using PCR-RFLP technique (Hph I). Heterozygotes AG were the
most frequent (37.5%) compared with AA (30.8%) and GG (31.7%) homozygotes. Observed
differences in total motility (TM), sperm membrane integrity (SYBR-14/PI) and ATP content
were significant between homozygotes AA or GG and heterozygotes AG. For all three traits disadvantageous
effect of heterozygotes AG was detected. This means that LPAR1 splicing mutation
has significant effect on semen quality and should be considered as a new marker of semen quality
in Holstein-Friesian bulls.
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