Despite significant changes of a political, legal and economic nature, despite clearly expressed intentions, the natural environment of most areas of Europe is further degraded. Therefore, it is necessary to adopt effective solutions, and one of the possibilities of determining the directions of support is the pro-ecological nature of the regional innovation strategy, which will allow transfer of much larger funds to support eco-innovation. The work is a case study. The aim of the research presented in this work is to show the relationship between the provisions of the Regional Innovation Strategy of the Podkarpackie Voivodeship for 2014-2020 for intelligent specialization (RIS3), and the assumptions of the five-helix concept, and the justifi cation for its use in future, in the form of a developed regional eco-innovation strategy.