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Abstract

Abstract In the previous study we obtained a population of interspecific F1 A. cepa × A. roylei hybrids. In this study, in comparison to the parental species: A. cepa and A. roylei, the F1 hybrids were evaluated in terms of plant morphology, pollen viability, microsporogenesis and female gametophyte. Most of the morphological characters of the F1 hybrids were intermediate as compared to those of both parental accessions. In pollen mother cells (PMCs) of the F1 hybrids abnormalities were observed in meiosis as well as at the tetrad stage. Pollen viability of F1 A. cepa × A. roylei hybrids was reduced to 30.1%. In the F1 hybrids, 45.8% of the analyzed ovules showed developmental disturbances, whereas in 26.7% of the ovules necrotic processes were observed.
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Authors and Affiliations

Alicja Chuda
Karolina Kłosowska
Adela Adamus
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Abstract

Abstract Prune dwarf virus (PDV) is a member of the Ilarvirus genus which is widely spread all over the world and causes considerable economic losses in nurseries and orchards. The virus is transmitted via seeds and pollen and through vegetative reproduction. However, the mechanisms of cell-to-cell and systemic transport of the virus are still not known. For the first time this study presents phylogenetic characterization of the movement protein (MP) of PDV isolates from the GenBank database in the context of geographic origin. The prepared analyses were based on a comparison of the whole amino acid sequence of the MP-PDV, the RNA-binding domain (RBD) in MP of PDV and MPs of four viruses from the Bromoviridae family with known transport mechanisms. Two different bioinformatic programs ClustalW and Jalview were used, and MP sequence variability up to 8% at the amino acid level among PDV isolates was confirmed. In the constructed phylogenetic trees the isolate sequences clustered in three conserved groups. Further analyses revealed similarity of the MP amino acid sequence of PDV and Alfalfa mosaic virus (AMV) of up to 34% and a 40% similarity of RBD between these viruses which suggested that the PDV transport mechanism may be on some level the same as that for AMV.
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Authors and Affiliations

Edmund Kozieł
Katarzyna Otulak
Grażyna Garbaczewska
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Abstract

Abstract Chromosome numbers for 16 Hieracium s.str. species from Bosnia and Herzegovina, Bulgaria, Macedonia, Montenegro, Poland, Romania and Serbia are given and their metaphase plates are illustrated. Chromosome numbers are published for the first time for H. filarszkyi Jáv. & Zahn 2n = 3x = 27, H. fritschianum Hayek & Zahn 2n = 3x = 27, H. fritzeiforme Zahn 2n = 3x = 27, H. hercegovinicum (Freyn & Vandas) Szeląg 2n=3x=27, H. nivimontis (Oborny & Zahn) Chrtek fil. 2n = 4x = 36, H. vagneri Pax 2n = 4x = 36, as well as three undescribed species of hybrid origin between H. olympicum Boiss. and H. sparsum Friv. 2n = 4x = 36, H. naegelianum Panc. and H. scardicum Bornm. & Zahn 2n = 3x = 27, and H. transylvanicum Heuff. and H. umbellatum L. 2n = 4x = 36.
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Authors and Affiliations

Krystyna Musiał
Zbigniew Szeląg
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Abstract

Abstract The aim of this paper is to elucidate relationship between anatomical changes and lignin deposition dynamics in the cell wall of barley (Hordeum vulgare L.) internodes during four growth stages: heading, flowering, grain filling and ripening. Microscopy was used to analyze anatomical changes related to lignin deposition whereas peroxidase activity was spectrophotometrically determined. In transversal sections lignin was found to be predominant in the sclerenchyma ring in cortex, where particularly lignified cell walls were found. Peroxidase activity was increased in older internodes and their localization in situ was in positive correlation with tissue lignification. Our results showed that, depending on the cultivar, at the flowering and grain filling stages intensive lignin synthesis and deposition occurred. This showed that deposition of lignin in the cell wall at a particular growth stage is in correlation with the lodging resistant phenotype of the investigated cultivars. The results contribute to the understanding of the lignin deposition process during barley development and consequential cell wall thickness.
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Authors and Affiliations

Lidija Begović
Jelena Ravlić
Hrvoje Lepeduš
Dunja Leljak-Levanić
Vera Cesar
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Abstract

Abstract The efficiency of phytoremediation might be highly affected by plant-associated microorganisms, and understanding of the underlying mechanisms is still a great challenge. The primary aim of this study was to evaluate the efficiency parameters for Cd2+ accumulation in the biomass of willow (Salix viminalis) as well as to define the biochemical response of the host plant when it is inoculated with selected bacterial strains (Massilia sp. and Pseudomonas sp.) or saprophytic fungus (Clitocybe sp.) under controlled in vitro conditions. Inoculation of plants with bacterial strains affected the efficiency of phytoremediation process and was expressed as the quantity of accumulated Cd (Q), the bioaccumulation factor (BCF) and the translocation index (Ti); however, the effect was strain and plant organ specific. The level of hydrogen peroxide (H2O2), which is both an indicator of plant response to biological and/or abiotic environmental stress and a molecule involved in plant-microbial interactions, decreased under the influence of Cd2+ in uninoculated plants (plant growth was inhibited by Cd2+) and increased in the inoculated variants of plants growing in the presence of Cd2+ (microbiologically stimulated biomass). The saprophytic fungus Clitocybe sp. generally stimulated biomass and increased the level of H2O2 synthesis in all the investigated plant organs and variants of the experiment. We suggest that determination of phytoremediation efficiency, and biochemical response (H2O2) of the host plant under in vitro conditions can help in predicting the final effect of plant-microbial systems in further field trials.
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Authors and Affiliations

Michał Złoch
Jarosław Tyburski
Katarzyna Hrynkiewicz
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Abstract

Abstract Soluble N-ethyl-maleimide sensitive factor attachment adaptor protein receptor (SNARE) domain-containing proteins were mainly involved in vesicle-associated membrane fusion. Genetic screening has revealed the function of SNARE in different aspects of plant biology. Among them, Synthaxin-22 (SYP22) a Qa-SNARE has been reported to have a pleiotropic function in plant development including regulation of leaf waving, shoot gravitropism and flowering time. In this study, we identified a new role of SYP22 in regulation of brassinosteroid (BR) signaling, especially in the dark. SYP22 interacts with BR receptor, brassinosteroid insensitive 1 (BRI1), and overexpression of SYP22 enhanced a weak BRI1 mutant bri1-5 phenotype. syp22 mutant exhibits short hypocotyl and it is sensitive to exogenously treated BR while slightly insensitive to BR-biosynthesis inhibitor propiconazole (PCZ) in the dark. Expression levels of BR signaling maker genes ACS5, SAUR15 and IAA19 were slightly higher, while BR6OX2, a BR biosynthesis marker gene, was lower in syp22 compared to the wild-type. In addition, syp22 was sensitive to 2,4-D, a synthetic auxin, in the dark. In conclusion, SYP22 is involved in BR- and auxin-mediated hypocotyl growth inhibition in the dark, which might be via interaction with BR and auxin key regulators to alter their internalization in Arabidopsis.
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Authors and Affiliations

Ting Shan Yao
Xiao Feng Zhu
Jin Hee Jung
Yuan Hu Xuan
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Abstract

Abstract Galinsoga quadriradiata Ruiz & Pav. is an annual weedy plant that can be found all over the world. It belongs to the Asteraceae family and is recognised as one of the invasive foreign plants in Poland, which are native to Central and South America. The aim of this study was to describe the reproductive features of Galinsoga quadriradiata focusing on the changes that occur during microsporogenesis and microgametogenesis along with the morphology of its pollen. As it is typical of the eudicot clade of Angiosperms, cytokinesis of G. quadriradiata is simultaneous. The pollen grains are tricolporate with spiny outer walls and the course of the microsporogenetic process is fairly typical of the Echinatae group of weed plants. The high viability of the pollen grains, which mature unequally in the inflorescences, and the proper course of meiosis determine whether a plant has the invasive character of Galinsoga quadriradiata.
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Authors and Affiliations

Jolanta Kolczyk
Monika Tuleja
Bartosz Jan Płachno
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Abstract

Abstract Plant tissue culture techniques have become an integral part of progress in plant science research due to the opportunity offered for close study of detailed plant development with applications in food production through crop improvement, secondary metabolites production and conservation of species. Because the techniques involve growing plants under controlled conditions different from their natural outdoor environment, the plants need adjustments in physiology, anatomy and metabolism for successful in vitro propagation. Therefore, the protocol has to be optimized for a given species or genotype due to the variability in physiological and growth requirement. Developing the protocol is hampered by several physiological and developmental aberrations in the anatomy and physiology of the plantlets, attributed to in vitro culture conditions of high humidity, low light levels and hetero- or mixotrophic conditions. Some of the culture-induced anomalies become genetic, and the phenotype is inherited by clonal progenies while others are temporary and can be corrected at a later stage of protocol development through changes in anatomy, physiology and metabolism. The success of protocols relies on the transfer of plantlets to field conditions which has been achieved with many species through stages of acclimatization, while with others it remains a challenging task. This review discusses various adjustments in nutrition, physiology and anatomy of micro-propagated plants and field grown ones, as well as anomalies induced by the in vitro culture conditions.
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Authors and Affiliations

Tasiu Isah
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Abstract

Abstract In vitro cultures of Anethum graveolens (dill) were maintained on the Linsmaier and Skoog (LS) medium – three variants, and the Murashige and Skoog (MS) medium – seven variants, which contained different amounts of plant growth regulators, cytokinin (BAP) and auxin (NAA) (from 0.1 mg l−1 to 3.0 mg l−1). Methanolic extracts from in vitro grown biomass were analyzed by HPLC for free phenolic acids and furanocoumarins. The total amounts of free phenolic acids on the LS medium variants were similar (35.23–38.65 mg 100 g−1 DW), but higher on the MS variants, ranging from about 66 mg 100 g−1 DW to 100 mg 100 g−1 DW. The main metabolites were: p-hydroxybenzoic acid (max. 24.41 mg 100 g−1 DW) on the LS−based media, and salicylic acid (max. 57.88 mg 100 g−1 DW) and p-hydroxybenzoic acid (max. 36.27 mg 100 g−1 DW) on the MS−based media. The total amounts of furanocoumarins were lower, as they did not exceed 8.5 mg 100 g−1 DW on the LS media and 25 mg 100 g−1 DW on the MS media. The main compounds in this group were bergapten (max. 15.01 mg 100 g−1 DW) and marmesin (max. 8.12 mg 100 g−1 DW). The MS variant containing 0.5 mg l−1 BAP and 2.0 mg l−1 NAA was proposed as the best production medium for both groups of metabolites. The maximum total amounts of free phenolic acids obtained in the in vitro grown biomass were slightly higher than their amounts in the fruits of the mother plant analyzed for comparison (99.66 mg 100 g−1 DW and 93.34 mg 100 g−1 DW, respectively); the maximum total amounts of furanocoumarins were approximately 1.8 times higher than in the fruits (24.26 mg 100 g−1 DW and 13.67 mg 100 g−1 DW, respectively).
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Authors and Affiliations

Agnieszka Szopa
Halina Ekiert
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Abstract

Abstract NH4+ is an important N-source which regulates plant growth and development. However, the underlying mechanism of NH4+ uptake and its-mediated signaling is poorly understood. Here, we performed phosphoproteomic studies using the titanium dioxide (TiO2)-mediated phosphopeptides collection method together with LC-MS analysis. The results indicated that phosphorylation levels of 23 and 43 peptides/proteins involved in diverse aspects, including metabolism, transport and signaling pathway, were decreased and increased respectively after NH4+ treatment in rice roots. Among 23 proteins detected, IDD10, a key transcription factor in ammonium signaling, was identified to reduce phosphorylation level of S313 residue. Further biochemical analysis using IDD10-GFP transgenic plants and immunoprecipitation assay confirmed that NH4+ supply reduces IDD10 phosphorylation level. Phosphorylation of ammonium transporter 1;1 (AMT1;1) was increased upon NH4+ treatment. Interestingly, phosphorylation of T446, a rice specific residue against Arabidopsis was identified. It was also established that phosphorylation of T452 is conserved with T460 of Arabidopsis AMT1;1. Yeast complementation assay with transformation of phosphomimic forms of AMT1;1 (T446/D and T452/D) into 31019b strain revealed that phosphorylation at T446 and T452 residues abolished AMT1;1 activity, while their plasma membrane localization was not changed. Our analyses show that many proteins were phosphorylated or dephosphorylated by NH4+ that may provide important evidence for studying ammonium uptake and its mediated signaling by which rice growth and development are regulated.
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Authors and Affiliations

Xiao Feng Zhu
Wan Hui Cai
Jin Hee Jung
Yuan Hu Xuan
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Abstract

Abstract There is increasing evidence showing that low selenium (Se) concentrations may increase tolerance of crop plants to several environmental stresses. The aim of this study was to compare the influence of two chemical forms of Se (selenite or selenate) at different concentrations (2 or 6 μM) on the resistance of butterhead lettuce (Lactuca sativa L. var. capitata) cv. Justyna to NaCl-induced stress (40 mM NaCl). Plant growth was negatively affected by salinity, but the level of photosynthetic pigments was not reduced. Se application at a concentration of 2 μM significantly improved the growth of salt-stressed plants, but selenite was much more effective than selenate in enhancing salt-tolerance of lettuce. The growth-promoting effect of Se was also noted at 6 μM of selenite, but did not appear at 6 μM of selenate. The beneficial effect of Se in salt-stressed lettuce could be due to antioxidative activity of Se, root system growth stimulation, and/or increase in photosynthetic pigment concentration after Se supplementation; however, it was not related to either increase in proline accumulation or reduction in foliar Na+ or Cl− concentration. These results imply that Se application, especially in the form of selenite, can enhance antioxidant defense of lettuce under salt stress, and Se supplementation may be recommended for areas of lettuce cultivation with excessive salt accumulation.
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Authors and Affiliations

Barbara Hawrylak-Nowak

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