Climate, land use, and land cover change can propagate alteration to the watershed environment. The interaction be-tween natural and human activities probably accelerates the change, a phenomenon that will generate serious environmental problems. This study aims to evaluate the change in the hydrological regime due to natural and human-induced processes. The study was conducted in Brantas watershed, Indonesia, which is the largest watershed in East Java. This area is populat-ed by more than 8 million inhabitants and is the most urbanized area in the region. An analysis of rainfall time series use to shows the change in natural phenomena. Two land-use maps at different time intervals were used to compare the rapid de-velopment of urbanization, and the discharge from two outlets of the sub-watersheds was employed to assess hydrological changes. The indicator of hydrological alteration (IHA) method was used to perform the analysis. The daily discharge data are from 1996 to 2017. The research results show an increase in flow (monthly, 1-day, 3-day, 7-day, 30-day, and 90-day flows) in the two sub-watersheds (Ploso and Kertosono) from the pre-period (1996–2006) to the post-period (2007–2017).