The article presents the research into hygienizing process of chicken manure using calcium peroxide (CaO₂) as an environmentally friendly biological deactivation agent. The influence of the addition of CaO₂ to chicken manure on the bioavailability of phosphorus was also analyzed. The process of biological deactivation using CaO₂, CaO and Ca(OH)₂ agents was analyzed applying the disk diffusion method. To optimize the effect of the hygienizing parameters, (CaO₂ concentration, pH, temperature and time) on the reduction of Enterobacteriaceae count the Taguchi method was applied. The content of bioavailable phosphorus was measured with the Egner-Riehm method and determined with spectrophotometry. The reduction in bacterial count followed an increase in the concentration of CaO₂ in a sample. The optimal experimental conditions (CaO₂=10.5 wt.%, pH=9.5, T=40°C, t=180 h) enabled a significant decrease in the Enterobacteriaceae count, from 10⁷ cfu/g to 10² cfu/g. Analysis of the samples with Egner-Riehm method showed that the phosphorus content decreased with the addition of biocide CaO₂: from 26.6 mg/l (for 3.5 wt.%) to 3.5 mg/l (for 10.5 wt.%). These values were slightly higher than the content of phosphorus deactivated with Ca(OH)₂ i.e., from 11.25 mg/l (for 3.5 wt.%) to 4.49 mg/l (for 10.5 wt.%). The application of CaO₂ for hygienizing chicken manure enables effective reduction of Enterobacteriaceae count to an acceptable level (below 1000 cfu/g). In comparison with the traditional techniques of hygienization, the application of CaO₂ has a positive effect on the recovery of bioavailable phosphorus.

The decolourization of Turquoise Blue HFG by immobilized cells of Lysinibacillus fusiformis B26 was investigated. Cells of L. fusiformis B26 were immobilized by entrapment in agar and calcium alginate matrices and attached in pumice particles. The effects of operational conditions (e.g., agar concentrations, cell concentrations, temperature, and inoculum amount) on microbial decolourization by immobilized cells were investigated. The results revealed that alginate was proven to be the best as exhibiting maximum decolourization (69.62%), followed by agar (55.55%) at 40°C. Pumice particles were the poorest. Optimum conditions for agar matrix were found: concentration was 3%, cell amount was 0.5 g and temperature was 40°C (55.55%). Ca-alginate beads were loaded with 0.5, 1.0 and 2.0 g of wet cell pellets and the highest colour removal activity was observed with 2.0 g of cell pellet at 40°C for alginate beads. Also, 0.5 and 1.0 g of pumice particles that were loaded with 0.25 and 0.5 g of cell pellets respectively were used and the results were found very similar to each other.

The Ca50Mg20Zn12Cu18 was assessed with different methods in order to characterize its basic characteristics, and to determine whether the amorphous alloy of such composition would be applicable as an implant material. The XRD analysis was conducted to conclude the structure of the initial material. The Ca50Mg20Zn12Cu18 ingot sample demonstrates crystalline structure containing two main intermetallic phases, however as-cast plates show features of an amorphous material, revealing the characteristic amorphous halo on the x-ray patterns. It was confirmed by the scanning electron microscopy method and fracture images revealing chevron pattern morphology with shell type fracture. Corrosion resistance, was studied using the potentiostatic analysis. The amorphous samples show higher resistance than the crystalline one. Post corrosion surface of the Ca50Mg20Zn12Cu18 alloy exhibits high concentration of magnesium and calcium hydroxides, forming the globular structures in large aggregates of spherical units.

The aim of this study was to evaluate the effect of high doses of calcium bentonite on the blood parameters, anticoccidial activity and intestinal histology of broiler chickens. Three undred and sixty one-day old broilers were distributed into three treatments (T+VE, T-VE, TB )with three replicates. Amprolium was added to the feed of the positive control group, calcium bentonite powder was added to the TB group, and nothing was added to the feed of the T-VE group. Coccidiosis was induced on day 14, the birds were kept until day 49, measurements of the diffe- rent variables started from week 3, blood samples were collected via wing vein, and fecal oocysts were counted from the intestinal contents of each individual bird using the McMaster techni- que. A decrease in feed consumption, body weight gain and conversion ratio was noticed in the calcium bentonite group. Broilers in the calcium bentonite group (TB ) and negative control group (T-VE ) showed clinical signs of coccidiosis (blood in feces) and the number of oocysts in feces increased with time. Histopathological examinations of the affected caeca also demonstrated excessive tissue damage, hemorrhage, the presence of clusters of large schizonts and merozoites in the tissue, and coccidian oocysts in the lumen. Feed conversion was highest in the T+VE group.

This article is devoted to basalt reprocessing together with magnetite concentrate in order to obtain ferrous alloy and calcium carbide. The studies have been based on thermodynamic simulation and electric smelting in arc furnace. The thermodynamic simulation has been performed using HSC-5.1 software based on the principle of minimum Gibbs energy. The blend was smelted in arc furnaces. On the basis of the obtained results of combined processing of basalt, it has been established that under equilibrium conditions, the increase in carbon content from 36 to 42 wt % of basalt and concentrate mixture makes it possible to increase the aluminum extraction into the alloy up to 81.4%, calcium into calcium carbide – up to 51.4%, and silicon into the alloy – up to 78.5%. Increase in the amount of lime to 32% allows to increase the content of calcium carbide to 278 dm3/kg. Electric smelting of the blend under laboratory conditions in the presence of 17-32% of lime makes it possible to extract ferrous alloy containing 69.5-72.8% of silicon, 69.1-70.2% of aluminum, and to obtain ferrous alloy containing 49-53% of ΣSi and Al and calcium carbide in the amount of 233-278 dm3/kg. During large-scale laboratory smelting of blend comprised of basalt (38.5%), magnetite concentrate (13.4%), lime (15.4%), and coke fines (32.7%), the ferrous alloy has been produced containing 48-53% of ΣSi and Al, calcium carbide in amount of 240-260 dm3/kg. Extraction of Si and Al into the alloy was 70.4 and 68.6%, respectively; Ca into carbide – 60.3%; Zn and Pb into sublimates – 99.6 and 92.8%, respectively.

Therefore, the aim of the present study was to evaluate the possible effect of bilberry fruit (Vaccinium myrtillus L.) supplement in a daily diet on the cognitive behaviour of the rats and the expression of paravalbumin (PV) in populations of hippocampal neurons. It has been postulated that the antioxidants present in bilberry fruit may act as neuroprotective factors playing also a significant role as memory enhancements. Forty Wistar rats with a similar average body weight (460 ± 0.4 g) were divided into four groups (n=10 per group). The control group received standard feed (210 g/week), whereas animals of experimental groups received standard feed supplemented with bilberry (per os) at consumed doses of 2 g (group I), 5 g (group II), and 10 g/kg b.w./ /day (group III). After three months of feeding with bilberry, the modified elevated plus-maze test (mEPM) was performed. After 32 weeks of feeding, brains were collected and PV-immunoreactive (ir) neurons were immunohistochemically visualized. In the modified elevated plus-maze test, transfer latency examined 2 h and 24 h after the acquisition session was significantly shorter (p<0.05) in the group II in comparison with the control group. In CA1 and CA2/CA3 hippocampal fields as well as dentate gyrus of all experimental groups, a significant (p<0.05) decrease in number of PV-ir neurons were found. In relation to the control group, the mean subpopulation of PV-ir neurons found in groups II and III were significantly reduced. The subpopulations of PV-ir neurons found in DG of all experimental groups were significantly reduced in comparison to the control. In conclusion the in the present paper we demonstrated a relationship between the diet rich in a bilberry fruit and process of memory as well as numbers of calcium- binding protein-expressing hippocampal neurons. Our results may be source of basic knowledge for further research aiming at neuroprotective role of the bilberry fruit.

The elemental composition and morphology of aerobic granules in sequencing batch reactors (GSBRs) treating high-nitrogen digester supernatant was investigated. The investigation particularly focused on the effect of the number of anoxic phases (one vs. two) in the cycle and the dose of external organics loading (450 mg COD/(L·cycle) vs. 540 mg COD/(L·cycle)) on granule characteristics. Granules in all reactors were formed of many single cells of rod and spherical bacteria. Addition of the second anoxic phase in the GSBR cycle resulted in enhanced settling properties of the granules of about 10.6% and at the same time decreased granule diameter of about 19.4%. The study showed that external organics loading was the deciding factor in the elemental composition of biomass. At 540 mg COD/(L·cycle) the granules contained more weight% of C, S and N, suggesting more volatile material in the granule structure. At lower organics loadings granules had the higher diameter of granules which limited the diffusion of oxygen and favored precipitation of mineral compounds in the granule interior. In this biomass higher content of Mg, P and Ca, was observed.

Effects of solution treatment on room temperature mechanical properties were studied in cast AZ91 (Mg-9%Al-1%Zn-0.2%Mn) and AZ91-0.5%Ca alloys. In as-cast state, the Ca addition contributed to the suppression of discontinuous β phase precipitation and the formation of Al2Ca phase. After solution treatment, the AZ91 alloy had only a small amount of Al8Mn5 particles, while β and Al2Ca phases were still present in the Ca-containing alloy. In as-cast state, the AZ91-0.5%Ca alloy showed better yield strength and hardness than the AZ91 alloy. The solution treatment increased the elongation in both alloys, which eventually led to the increase in ultimate tensile strength. The solution treatment resulted in a marked decrease in yield strength and hardness in the AZ91 alloy, whereas the decrements in those values were relatively negligible in the Ca-containing alloy due to the residual phases and solution hardening effect of Ca.

The general objective of this research has been to identify the factors and conditions of migration of CaCO3 within glaciers and their marginal zones in Svalbard . Special attention has been paid to the cryochemical processes responsible for precipitation of calcium carbonate in icing (naled ice) formed near fronts of polythermal glaciers during winter. Estimates of the importance of those processes in respect of the general mineral mass transfer in the glacier system are attempted here. Field studies concerning the carbonate contents in proglacial sediments and icing fields were carried out in the Werenskioldbreen and the Elisebreen basins (S and NW Spitsbergen respectively). A functional model of CaCO3 migration in a glacier system is proposed which indicates the various paths of the mineral mass flow. Considerations on intensity of glacial processes permitted quantitative estimation of the particular components in respect to the Werenskioldbreen basin. Cryochemical processes do not appear to be of overriding importance in such migration but, clearly, they play a specific role in retaining CaCO3 in the proglacial zone on land. The crystalline forms present in the icings, which have many lattice defects, are very easily re-dissolved or removed by wind.

Shoot tips excised from shoot culture of Salvia officinalis were encapsulated in 2% or 3% (w/v) sodium alginate and exposed to 50 mM calcium chloride for complexation. Immediately or after 6, 12 or 24 weeks of storage at 4°C, the synthetic seeds were cultured for 6 weeks on half-strength MS medium supplemented with indole-3-acetic acid (IAA) (0.1 mg/l) and solidified with 0.7% agar. The frequency of shoot and root emergence from encapsulated shoot tips was affected by the concentrations of sodium alginate and additives in the gel matrix (sucrose, gibberellic acid, MS nutrient medium) as well as duration of storage. The frequency of shoot and root induction of non-stored synthetic seeds was highest with shoot tips encapsulated with 2% sodium alginate containing 1.5% sucrose and 0.5 mg/l gibberellic acid (GA3). Shoot tips maintained their viability and ability to develop shoots even after 24 weeks of storage when they were encapsulated in 3% alginate with 1/3 MS medium, sucrose (1.5%) and GA3 (0.25 mg/l). Root formation tended to decrease with storage time. Overall, 90% of the plantlets derived from stored and non-stored synthetic seeds survived in the greenhouse and grew to phenotypically normal plants. This procedure can enable the use of synthetic seed technology for germplasm conservation of S. officinalis, a plant species of high medical and commercial value.