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Phytoremediation of phenol using Polygonum orientale and its antioxidative response

Kai Wang Jin Cai Shulian Xie Jia Feng Ting Wang

Archives of Environmental Protection | 2015 | vol. 41 | No 3 | DOI: 10.1515/aep-2015-0024

Keywords Polygonum orientale phenol phytoremediation antioxidant enzyme osmotic regulator

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Abstract

Polygonum orientale with beautiful red flowers can be found as one dominant species in the vicinity of most water bodies and wetlands in China. However, its phytoremediation potential has not been sufficiently explored because little is known about its resistance to inorganic or organic pollutants. We investigated P. orientale response to low and moderate levels of phenol stress (≤ 80 mg L-1). Endpoints included phenol tolerance of P. orientale and the removal of the pollutant, antioxidant enzyme activities, damage to the cell membrane, osmotic regulators and photosynthetic pigments. In plant leaves, phenol stress significantly increased the activities of peroxidase (POD) and catalase (CAT), as well as the contents of proline, soluble sugars and carotenoids, whereas superoxide dismutase (SOD), H2O2 and electrolyte leakage (EL) levels remained unaltered. On the other hand, there were significant decreases of soluble protein and chlorophyll contents. We demonstrated that, in combination with phenol tolerance and its removal, P. orientale has efficient protection mechanisms against phenol-induced oxidative damage (≤ 80 mg L-1). We propose that P. orientale could be used as an alternative and interesting material in the phytoremediation of phenol.

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Authors and Affiliations

Kai Wang

Jin Cai

Shulian Xie

Jia Feng

Ting Wang

Missense mutation within cystic fibrosis transmembrane conductance regulator (CFTR) gene is associated with selected parameters of the frozen-thawed spermin Holstein-Friesian bulls

S. Kaminski D.M. Hering W. Kordan M. Lecewicz

Polish Journal of Veterinary Sciences | 2019 | vol.22 | No 2 | 221-225 | DOI: 10.24425/pjvs.2019.127089

Keywords bull sperm polymorphism CFTR gene antioxidant enzyme activity

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Abstract

In our previous Genome-wise Association Study we found that Cystic Fibrosis Transmem- brane Conductance Regulator gene (CFTR) is a candidate gene for sperm motility in fresh semen of Holstein-Friesian bulls. Since in cows thawed semen is commonly used for the artificial insem- ination (AI) we have decided to find out whether functional polymorphism within CFTR gene coding sequence is associated with selected parameters of thawed sperm, including their motility evaluated by computer-assisted sperm analysis (CASA), the activity of three antioxidant enzymes: glutathione peroxidase (GPx) catalase (CAT), superoxide dismutase (SOD), ATP con- tent and integrity of sperm membranes. One hundred twenty Holstein Friesian bulls kept in uni- form environmental conditions (one AI company) were included in the study. Significant associ- ations between genotypes of missense mutation within exon 11 of the CFTR gene (Met468Leu) and the activity of antioxidant enzymes and sperm mitochondrial function were revealed. No effect of CFTR genotypes on sperm motility was observed. Significant differences in CAT and SOD activity were found between AA and TT homozygous individuals. Bulls with TT genotype had the lowest activity of both antioxidant enzymes. The same bulls also showed the lowest num- ber of sperm with active mitochondria. Our results demonstrate that missense mutation Met468Leu within CFTR gene is associated with antioxidant enzyme activity and mitochondrial function of bovine thawed sperm without affecting their motility.

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Authors and Affiliations

S. Kaminski

D.M. Hering

W. Kordan

M. Lecewicz

Alleviating Effect of Melatonin on Zinc-Copper Stressed Seedlings of Hemp ( Cannabis sativa L.)

Hakimeh Oloumi Ali Zamani Hossein Mozaffari Seyyed Mohammad Javad Arvin Hassan Salari

Acta Biologica Cracoviensia s. Botanica | 2022 | Vol. 64 | No 2 | 49-64 | DOI: 10.24425/abcsb.2022.143382

Keywords antioxidant enzymes cannabis mineral nutrition plant growth stimulator

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Abstract

The study was carried out to investigate the interactive effects of exogenous melatonin and excess amounts of zinc and copper on the growth and physiological parameters, antioxidant defense system and nutritional balance of cannabis seedlings. Cannabis sativa L. plants, grown under a completely randomized design, were irrigated with complete Hoagland’s nutrient solution. CuSO ₄ (0, 50 and 150 µM) and ZnSO ₄ (0, 50 and 100 µM) and their combinations were supplied to 21-day-old seedlings for 2 weeks. During the second week, melatonin was added to the nutrient solution at 100 μM. Zn and Cu stress led to reduced growth and physiological parameters, it promoted oxidative stress, changes in antioxidant enzymes activity and imbalance of mineral nutrients in cannabis seedlings. However, melatonin alleviated the growth retardation and physiological disorders of seedlings under normal conditions and heavy metal stress. The content of reduced glutathione and the activity of antioxidant enzymes such as glutathione reductase and ascorbate peroxidase were improved by melatonin. Excess amounts of zinc and copper changed the pattern of nutritional elements distribution in cannabis seedlings. Cu and Zn caused reduced content of Fe, Ca and K ions in shoots and roots. Melatonin treatment was able to adjust the nutrients content in metal-stressed seedlings up to the level of the control. Exogenous melatonin reduced toxic levels of Cu and Zn in seedlings overloaded with copper and zinc. MT also raised K, Ca and Fe concentrations in roots and shoots of seedlings under stress. Our results support the idea that melatonin acts as a powerful antioxidant, it can also be considered as a potent regulator of ion homeostasis in cannabis seedlings under heavy metal toxicity. Further studies still need to investigate the alleviatory effects of melatonin under field conditions.

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Authors and Affiliations

Hakimeh Oloumi

Ali Zamani

Hossein Mozaffari

Seyyed Mohammad Javad Arvin

Hassan Salari

Department of Ecology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
Plant Products Division, Shahid Bahonar University, Kerman, Iran

Neuro-protective and redox potential of troxerutin against cypermethrin-induced neurotoxicity and oxidative stress in mice

S. Shehzad Z. Farooq S.K. Tahir M.I. Masood S.M.M. Anjum A.A. Saeed M.S. Yousaf K.A. Majeed I. Rabbani S. Basharat

Polish Journal of Veterinary Sciences | 2024 | vol. 27 | No 2 | 289-299 | DOI: 10.24425/pjvs.2024.149362

Keywords antioxidant enzymes behavioral tests oxidant level pesticides rodents watersoluble flavonoids

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Abstract

The present study was designed to evaluate the protective efficacy of troxerutin against cypermethrin-induced behavioral defects, motor function abnormalities, and oxidative stress in mice. Twenty-four adult female albino mice were randomly divided into four equal groups. The first group served as control, the second group was treated with cypermethrin (20 mg/kg b.w) intraperitoneally at day 21, and the remaining two groups were orally supplemented with TRX (150, 300 mg/kg b.w) for 20 days and with cypermethrin (20 mg/kg b.w) intraperitoneally at day 21. Behavior activities recorded after cypermethrin exposure showed significantly impaired motor function (p≤0.05) as evidenced by the beam balance and pole test. The cypermethrin was also found to cause significant memory dysfunction. Moreover, the oxidative stress in terms of increased tissue malondialdehyde level (p≤0.05) was recorded in the cypermethrin group. The antioxidant activities of catalase and glutathione peroxidase were decreased (p≤0.05) after cypermethrin exposure. Troxerutin supplementation significantly improved the cypermethrin- -induced motor impairment and memory dysfunction. The supplementation of troxerutin significantly restored the redox status. Troxerutin attenuates the neurotoxic and behavioral deficits caused by cypermethrin. Furthermore, troxerutin also provides significant protection against cypermethrin-induced oxidative stress by improving the oxidative stress markers.

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Bibliography

Ali HF, El Sayed NM, Ahmed AA, Hanna PA, Moustafa YM (2020) Nano selenium ameliorates oxidative stress and inflammatory response associated with cypermethrin-induced neurotoxicity in rats. Ecotoxicol Environ Saf 195: 110479.

Ateşşahin A, Yilmaz S, Karahan I, Pirinçci I, Taşdemir B (2005) The effects of vitamin E and selenium on cypermethrin-induced oxidative stress in rats. Turk J Vet Anim Sci 29: 385-391.

Azarfarin M, Farajdokht F, Babri S, Salehpour F, Taghizadeh M, Mohaddes G (2018) Effects of troxerutin on anxiety- and depressive-like behav-iors induced by chronic mild stress in adult male rats. Iran J Basic Med Sci 21: 781-786.

Badalzadeh R, Chodari L, Ghorbanzadeh V (2017) Troxerutin, a bioflavonoid, improves oxidative stress in blood of streptozotocin-induced type-1 diabetic rats. Iran J Pharm Sci 13: 75-86.

Bali YA, Kaikai NE, Ba-M’hamed S, Bennis M (2019) Learning and memory impairments associated to acetylcholinesterase inhibition and oxida-tive stress following glyphosate based-herbicide exposure in mice. Toxicology 415: 18-25.

Baluchnejadmojarad T, Jamali-Raeufy N, Zabihnejad S, Rabiee N, Roghani M (2017) Troxerutin exerts neuroprotection in 6-hydroxydopamine lesion rat model of Parkinson’s disease: Possible involvement of PI3K/ERβ signaling. Eur J Pharmacol 801: 72-78.

Bayandor P, Farajdokht F, Mohaddes G, Diba R, Hosseindoost M, Mehri K, Zavvari Oskuye Z, Babri S (2019) The effect of troxerutin on anxie-ty-and depressive-like behaviours in the offspring of high-fat diet fed dams. Arch Physiol Biochem 125: 156-162.

Burton GW, Cheeseman KH, Ingold KU, Slater TF (1983) Lipid antioxidants and products of lipid peroxidation as potential tumour protective agents. 261-262.

Colle D, Santos DB, Naime AA, Gonçalves CL, Ghizoni H, Hort MA, Farina M (2020) Early postnatal exposure to paraquat and maneb in mice increases nigrostriatal dopaminergic susceptibility to a Re-challenge with the same pesticides at adulthood: Implications for Parkinson’s dis-ease. Neurotox Res 37: 210-226.

Feldman E (2004) Thiobarbituric acid reactive substances (TBARS) assay. AMDCC Protocols Version. 1.

Flohé L, Günzler WA (1984) Assays of glutathione peroxidase. Methods Enzymol 105: 114-120.

Gabbianelli R, Nasuti C, Falcioni G, Cantalamessa F (2004) Lymphocyte DNA damage in rats exposed to pyrethroids: effect of supplementation with Vitamins E and C. Toxicology 203: 17-26.

Gao C, Song Y, Dou T, Jiang S, Wu H, Seshadri VD, Veeraraghavan VP, Hou P (2021) Troxerutin abrogates ischemic/reperfusion-induced brain injury through ameliorating oxidative stress and neuronal inflammation by inhibiting the expression of NLRP3 in sprague dawley rats. J Envi-ron Pathol Toxicol Oncol 40: 11-19.

Garabadu D, Agrawal N (2020) Naringin exhibits neuroprotection against rotenone-induced neurotoxicity in experimental rodents. Neuromolecular Med 22: 314-330.

Hadwan MH, Abed HN (2016) Data supporting the spectrophotometric method for the estimation of catalase activity. Data Brief 6: 194-199.

Hussien HM, Abdou HM, Yousef MI (2013) Cypermethrin induced damage in genomic DNA and histopathological changes in brain and haema-totoxicity in rats: the protective effect of sesame oil. Brain Res Bull 92: 76-83.

Hussein RM, Mohamed WR, Omar HA (2018) A neuroprotective role of kaempferol against chlorpyrifos-induced oxidative stress and memory deficits in rats via GSK3β-Nrf2 signaling pathway. Pestic Biochem Physiol 152: 29-37.

Ince S, Kucukkurt I, Demirel HH, Turkmen R, Sever E (2012) Thymoquinone attenuates cypermethrin induced oxidative stress in Swiss albino mice. Pestic Biochem Physiol 104: 229-235.

Jamali-Raeufy N, Kardgar S, Baluchnejadmojarad T, Roghani M, Goudarzi M (2019) Troxerutin exerts neuroprotection against lipopolysaccharide (LPS) induced oxidative stress and neuroinflammation through targeting SIRT1/SIRT3 signaling pathway. Metab Brain Dis 34: 1505-1513.

Knippenberg S, Thau N, Dengler R, Petri S (2010) Significance of behavioural tests in a transgenic mouse model of amyotrophic lateral sclerosis (ALS). Behav Brain Res 213: 82-87.

Li H, Xie YH, Yang Q, Wang SW, Zhang BL, Wang JB, Cao W, Bi LL, Sun JY, Miao S (2012) Cardioprotective effect of paeonol and danshensu combination on isoproterenol-induced myocardial injury in rats. PloS One 7: e48872. Luong TN, Carlisle HJ, Southwell A, Patterson PH (2011) Assessment of motor balance and coordination in mice using the balance beam. J Vis Exp e2376.

Madiha S, Batool Z, Tabassum S, Liaquat L, Sadir S, Shahzad S, Naqvi F, Saleem S, Yousuf S, Nawaz A (2021) Quercetin exhibits potent anti-oxidant activity, restores motor and non-motor deficits induced by rotenone toxicity. Plos One 16: e0258928.

Mahmood I, Imadi SR, Shazadi K, Gul A, Hakeem KR (2016) Effects of pesticides on environment. In: Hakeem K, Akhtar M, Abdullah S. (eds) Plant, soil and microbes. Springer, Cham. 253-269.

Meister A (1974) Glutathione; Metabolism and function via the gamma-glutamyl cycle. Life Sci 15: 177-190.

Mezni A, Mhadhbi L, Khazri A, Sellami B, Dellali M, Mahmoudi E, Beyrem H (2020) The protective effect of Hibiscus sabdariffa calyxes extract against cypermethrin induced oxidative stress in mice. Pestic Biochem Physiol 165: 104463.

C, Brunori G, Eusepi P, Marinelli L, Ciccocioppo R, Gabbianelli R (2017) Early life exposure to permethrin: a progressive animal model of Parkinson’s disease. J Pharmacol Toxicol Methods 83: 80-86.

Raja B, Saranya D, Prabhu R (2019) Role of flavonoid troxerutin on blood pressure, oxidative stress and regulation of lipid metabolism. Front Biosci (Elite Ed) 11: 121-129.

Rajawat NK, Soni I, Syed F, Verma R, John PJ, Mathur R (2019) Effect of β-cyfluthrin (synthetic pyrethroid) on learning, muscular coordination and oxidative stress in Swiss albino mice. Toxicol Ind Health 35: 358-367.

Righi DA, Palermo-Neto J (2003) Behavioral effects of type II pyrethroid cyhalothrin in rats. Toxicol Appl Pharmacol 191: 167-176.

Sankar P, Telang AG, Manimaran A (2012) Protective effect of curcumin on cypermethrin-induced oxidative stress in Wistar rats. Exp Toxicol Pathol 64: 487-493.

Sharma P, Firdous S, Singh R (2014) Neurotoxic effect of cypermethrin and protective role of resveratrol in Wistar rats. Int J Nutr Pharmacol Neurol Dis 4: 104-111.

Tian YT, Liu ZW, Yao Y, Zhang T, Yang Z (2008) Effects of alpha and theta-cypermethrin insecticide on transient outward potassium current in rat hippocampal CA3 neurons. Pestic Biochem Physiol 90: 1-7.

Wu Y, Jiao Z, Wan Z, Qu S (2021) Role of autophagy and oxidative stress to astrocytes in fenpropathrin-induced Parkinson-like damage. Neuro-chem Int 145: 105000.

Yadav A, Tandon A, Seth B, Goyal S, Singh SJ, Tiwari SK, Agarwal S, Nair S, Chaturvedi RK (2021) Cypermethrin Impairs Hippocampal Neu-rogenesis and Cognitive Functions by Altering Neural Fate Decisions in the Rat Brain. Mol Neurobiol 58: 263-280.

Yaqub A, Faheem I, Anjum KM, Ditta SA, Yousaf MZ, Tanvir F, Raza C (2020) Neurotoxicity of ZnO nanoparticles and associated motor func-tion deficits in mice. Appl Nanosci 10: 177-185.

Zamanian M, Hajizadeh MR, Esmaeili Nadimi A, Shamsizadeh A, Allahtavakoli M (2017) Antifatigue effects of troxerutin on exercise endurance capacity, oxidative stress and matrix metalloproteinase‐9 levels in trained male rats. Fundam Clin Pharmacol 31: 447-455.

Ziada RM, Nahas AA, Farag A, Kotb GA (2020) Protective Efficacy of Combined Administration of Vitamins C and Curcumin on Cyperme-thrin-Induced Oxidative Stress in Male Albino Rats. Egypt Acad J Biol Sci F Toxicol Pest Control 12: 109-118.

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Authors and Affiliations

S. Shehzad

Z. Farooq

S.K. Tahir

M.I. Masood

S.M.M. Anjum

A.A. Saeed

M.S. Yousaf

K.A. Majeed

I. Rabbani

S. Basharat

Department of Physiology, University of Veterinary and Animal Sciences, Syed Abdul Qadir Jilani (Out Fall) Road, Lahore 54000, Pakistan
Institute of Pharmaceutical Sciences, University of Veterinary and Animal Sciences, Syed Abdul Qadir Jilani (Out Fall) Road, Lahore 54000, Pakistan

Cell response of Antarctic strain Penicillium griseofulvum against low temperature stress

Ekaterina Ts. Krumova Ekaterina K. Koeva Stoyanka R. Stoitsova Tsvetelina S. Paunova-Krasteva Galina D. Stoyancheva Maria B. Angelova

Polish Polar Research | 2022 | vol. 43 | No 2 | 125-143 | DOI: 10.24425/ppr.2021.138587

Keywords Antarctic filamentous fungi oxidative stress biomarkers antioxidant enzymes

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Abstract

Duringthe evolution organisms are subjected to the continuous impact of environmental factors. In recent years an increasing number of studies have focused on the physicochemical limits of lifeon Earthsuch as temperature, pressure, drought, salt content, pH, heavy metals, etc. Extreme environmental conditions disrupt the most important interactions that support the function and structure of biomolecules.Forthis reason,organisms inhabiting extreme habitats have recently become of particularlygreat interest. Although filamentous fungi are an important partof the polar ecosystem, information about their distribution and diversity, as well as their adaptation mechanisms, is insufficient. In the present study,the fungal strain Penicillium griseofulvum isolated from an Antarctic soil sample was used as a study model. The fungal cellular response against short term exposure to low temperature was observed. Our results clearly showed that short-term low temperature exposure caused oxidative stress in fungal cells and resulted in enhanced level of oxidative damaged proteins, accumulation of reserve carbohydrates and increased activity of the antioxidant enzyme defence. Ultrastructural changes in cell morphology wereanalysed. Different pattern of cell pathology provoked by the application of two stress temperatures was detected. Overall, this study aimed to observe the survival strategy of filamentous fungi in extremely cold habitats, and to acquire new knowledge about the relationship between low temperature and oxidative stress.

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Authors and Affiliations

Ekaterina Ts. Krumova

Ekaterina K. Koeva

Stoyanka R. Stoitsova

Tsvetelina S. Paunova-Krasteva

Galina D. Stoyancheva

Maria B. Angelova

The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26, Acad. G. Bonchev str., 1113 Sofia, Bulgaria

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Phytoremediation of phenol using Polygonum orientale and its antioxidative response

Abstract

Authors and Affiliations

Missense mutation within cystic fibrosis transmembrane conductance regulator (CFTR) gene is associated with selected parameters of the frozen-thawed spermin Holstein-Friesian bulls

Abstract

Authors and Affiliations

Alleviating Effect of Melatonin on Zinc-Copper Stressed Seedlings of Hemp ( Cannabis sativa L.)

Abstract

Authors and Affiliations

Neuro-protective and redox potential of troxerutin against cypermethrin-induced neurotoxicity and oxidative stress in mice

Abstract

Bibliography

Authors and Affiliations

Cell response of Antarctic strain Penicillium griseofulvum against low temperature stress

Abstract

Authors and Affiliations