Abstract
The aim of this study was to measure the NO level in boar semen held in a liquid state and to
determine its putative relation to spermatozoa motility, plasma membrane integrity, mitochondrial
membrane potential and ATP content. Generally, the percentage of spermatozoa which
generated nitric oxide gradually increased, while NO level in the surrounding medium declined
during the liquid preservation. NO generation in semen preserved in BTS was higher as compared
to those in Androhep®Plus. We demonstrated the positive correlation between the NO
level in fresh spermatozoa and their quality. We also showed negative correlation between nitric
oxide level in spermatozoa preserved in BTS and sperm cells motility as well as plasma membrane
integrity. Results obtained in this study confirm that NO may affect sperm physiology in a dualistic
manner.
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