Search results

Filters

  • Journals
  • Authors
  • Keywords
  • Date
  • Type

Search results

Number of results: 9
items per page: 25 50 75
Sort by:
Download PDF Download RIS Download Bibtex

Abstract

Nitritation, the first stage of ammonia removal process is known to be limiting for total process performance. Ammonia oxidizing bacteria (AOB) which perform this process are obligatory activated sludge habitants, a mixture consisting of Bacteria, Protozoa and Metazoa used for biological wastewater treatment. Due to this fact they are an interesting bacterial group, from both the technological and ecological point of view. AOB changeability and biodiversity analyses both in wastewater treatment plants and lab-scale reactors are performed on the basis of 16S rRNA gene sequences using PCR-DGGE (Polymerase Chain Reaction – Denaturing Gradient Gel Electrophoresis) as a molecular biology tool. AOB researches are usually led with nested PCR. Because the application of nested PCR is laborious and time consuming, we have attempted to check the possibility of using only first PCR round to obtain DGGE fingerprinting of microbial communities. In this work we are comparing the nested and non-nested PCR-DGGE monitoring of an AOB community and presenting advantages and disadvantages of both methods used. The experiment revealed that PCR technique is a very sensitive tool for the amplification of even a minute amount of DNA sample. But in the case of nested-PCR, the sensitivity is higher and the template amount could be even smaller. The nested PCR-DGGE seems to be a better tool for AOB community monitoring and complexity research in activated sludge, despite shorter fragments of DNA amplification which seems to be a disadvantage in the case of bacteria identification. It is recommended that the sort of analysis approach should be chosen according to the aim of the study: nested-PCR-DGGE for community complexity analysis, while PCR-DGGE for identification of the dominant bacteria.
Go to article

Authors and Affiliations

Aleksandra Ziembińska-Buczyńska
Jarosław Wiszniowski
Sławomir Ciesielski
Download PDF Download RIS Download Bibtex

Abstract

A filamentous benthic cyanobacteria, strain USMAC16, was isolated from the High Arctic Svalbard archipelago, Norway, and a combination of morphological, ultrastructural and molecular characterisation (16S rRNA gene sequence) used to identify to species level. Cell dimensions, thylakoid arrangement and apical cell shape are consistent with the Pseudanabaena genus description. The molecular characterisation of P. catenata gave 100% similarity with Pseudanabaena catenata SAG 1464-1, originally reported from Germany. Strain USMAC16 was cultured under a range of temperature and photoperiod conditions, in solid and liquid media, and harvested at exponential phase to examine its phenotypic plasticity. Under different culture conditions, we observed considerable variations in cell dimensions. The longest cell (5.91±0.13 μm) was observed at 15°C under 12:12 light:dark, and the widest cell (3.24±0.06 μm) at 4°C under 12:12 light: dark in liquid media. The study provides baseline data documenting the morphological variation of P. catenata in response to changing temperature regimes.
Go to article

Authors and Affiliations

Jerzy Smykla
Zoya Khan
Wan Maznah Wan Omar
Faradina Merican Mohd Sidik Merican
Asmimie Asmawarnie Azizan
Choon Pin Foong
Peter Convey
Nazalan Najimudin
Siti Aisyah Alias
Download PDF Download RIS Download Bibtex

Abstract

Phenol degradation efficiency or Pscndontonas putida PCM2 l 53 free cel ls was experimentally studied. Bacterial cells were acclimatized to phenol what relied on gradually increasing the phenol concentration in the medium. The highest phenol degradation rate was calculated as approximately 15.2 mgdmŁh'. Investigated strain degraded the phenol at the concentration or 400 111g-d111·-' in 24 h. The result or toxicity analysis showed that acclimatized cells orP putida PCM2 l 53 arc able to survive even al as high concentration or phenol as 3000 rng.dm'. The obtained result suggests that the analyzed strain can be used lor cflcciivc treating of high strength phenolic wastewater. Due to resistance of the strain to high phenol concentration it may be applied in biorcmcdiation of exceedingly contaminated sites, especially where dilution or pollutants cannot be implemented.
Go to article

Authors and Affiliations

Grzegorz Przybyłek
Sławomir Ciesielski
Download PDF Download RIS Download Bibtex

Abstract

Polycyclic aromatic hydrocarbons (PAHs) belong to the group of recalcitrants that on reaching wastewater can irreversibly inhibit some sensitive biological processes in activated sludge such as nitrification. This situation leads to wastewater treatment failure due to the influence of these substances on bacteria responsible for important biochemical processes. Observation of the changes in bacterial diversity using molecular tools, such as denaturing gradient gel electrophoresis (DGGE), could be the first step in finding a way of preventing wastewater treatment failure. The aim of this experiment was to monitor bacterial biodiversity in a membrane bioreactor (MBR) dealing with synthetic wastewater contaminated with high concentration of petroleum organic compounds (POCs) and to study the influence of POCs contamination on bacterial changeability in activated sludge. COD removal in investigated membrane bioreactors was at a level of 93%. The organics removal efficiency was not affected by the maximal tested dose of petroleum contamination ( l OOO μl POCs/l of wastewater) and the MBRs wastewater treatment performance was undisturbed. DGGE analysis revealed that the biodiversity fluctuated slightly in control MBR, while in experimental MBR the biodiversity index decreased drastically after adding the highest experimental concentration of POCs. These results suggest that concentrations of POCs at levels from 50 μl/l to 500 μl/l stimulate biodiversity growth, while the concentration I OOO μI POCs/1 of wastewater seems to inhibit the most sensitive processes in wastewater treatment by influencing the bacterial biocenosis.
Go to article

Authors and Affiliations

Aleksandra Ziembińska
Sławomir Ciesielski
Jarosław Wiszniowski
Download PDF Download RIS Download Bibtex

Abstract

The soil microbiome is exposed to technogenic influence during the operation of metal structures. There are quantitative and qualitative changes in the microbiota of the technogenic ecosystem. During the study of the technogenic soil ecosystem (ferrosphere), samples of which were taken in the field (Chernihiv, Ukraine: 51°29’58”N, 31°16’09”E), the presence of corrosively active microbial cenosis was established: sulfate-reducing, denitrifying, iron-reducing (using acetate as the only electron donor, and Fe (III) as the only electron acceptor) and ammonifying bacteria. The predominant representatives of corrosively active groups of bacteria were isolated. They were identified as Bacillus simplex, Streptomyces gardneri, Streptomyces canus (ammonifying bacteria), Fictibacillus sp. (ammonifying bacteria with iron-reducing ability), Anaerotignum (Clostridium) propionicum (organic acid-producing bacteria), Desulfovibrio oryzae (sulfate-reducing bacteria) based on some microbiological, physiological and biochemical, genetic features. Strains of heterotrophic and hemolitotrophic bacteria (individual representatives and their associations) isolated from the technogenic ecosystem can be used in both industrial and technological spheres. The interaction of isolated bacteria in the process of microbial induced corrosion is a prospect for further research.
Go to article

Bibliography

Abdulina, D.R., Asaulenko, L.G., Purish, L.M., 2011. Dissemination of corrosive aggressive bacteria in soils of different biotopes (Rozpovsiudzhennia koroziino-ahresyvnykh bakterii u gruntakh riznykh biotopiv). Studia Biologica 5 (1), 11–16. (in Ukrainian).

Agrawal, A., Vanbroekhoven, K., Lal, B., 2010. Diversity of culturable sulfidogenic bacteria in two oil-water separation tanks in the north-eastern oil fields of India. Anaerobe 16 (1), 12–18.

Ait-Langomazino, N., Sellier, R., Jonquet, G., Trescinski, M., 1991. Microbial degradation of bitumen. Experientia 6, 533–539.

AlAbbas, F.M., Williamson, Ch., Bhola, Sh.M., Spear, J.R., Olson, D.L., Mishra, B., Kakpovbia, A.E., 2013. Microbial Corrosion in Linepipe Steel Under the Influence of a Sulfate-Reducing Consortium Isolated from an Oil Field. Journal of Materials Engineering and Performance 22 (11), 3517–3529.

Amann, R.J., Stromley, J., Devereux, R., Key, R., Stahl, D.A., 1992. Molecular and microscopic identification of sulfate-reducing bacteria in multispecies biofilms. Applied and Environmental Microbiology 58, 614–623.

Andreyuk, E.I., Kozlova, I.A., Kopteva, Zh.P., Pilyashenko-Novokhatny, A.I., Zanina, V.V., Purish, L.M., 2002. Ferrosphere – formation zone corrosive community of microorganisms (Ferrosfera – zona formirovaniya korrozionno-aktivnogo soobschestva mikroorganizmov). Reports of the NAS of Ukraine 3, 157–161. (in Russian)

Andreyuk, K., Kozlova, I., Koptieva, Zh., Pilyashenko-Novokhatny, A., Zanina, V., Purish, L., 2005. Microbial Corrosion of Underground Structures, Naukova Dumka, Kyiv. (in Ukrainian with English summary).

Antonovskaya, N.S., Kozlova, I.A., Andreyuk, E.I., 1986. Thiobacillus thioparus – active agent in steel corrosion. Mikrobiologii Zhurnal 1, 36–41. (in Russian with English summary)

Aseeva, I.V., Babieva, I.P., Byzov, B.A., Goosev, V.S., Dobrovolskaya, T.G., Zvyagintsev, D.G., Zenova, G.M., Kozhevin, P.A., Kurakov, A.V., Lysak, L.V., Marfenina, O.E., Mirchink, T.G., Polyanskaya, L.M., Panikov, N.S., Skvortsova, I.N., Stepanov, A.L., Umarov, M.M., 1991. Methods of soil microbiology and biochemistry (Metodyi pochvennoy mikrobiologii i biohimii). In: Zvyagintsev, D.G. (Ed.), Moscow University Press, Moscow. (in Russian).

Bala, D.D., Chidambaram, D., 2014. Effect of anaerobic microbial corrosion on the surface film formed on steel. ECS Transactions 58 (41), 137–149.

Bano, A.Sh., Qazі, J.I., 2011. Soil Buried Mild Steel Corrosion by Bacillus cereus-SNB4 and its Inhibition by Bacillus thuringiensis- SN8. Pakistan Journal of Zoology 43 (3), 555–562.

Bergey’s Manual of Systematic Bacteriology, 2005. Second Edition, Volume 2, The Proteobacteria, Part C. The Alpha-, Beta-, Delta-, and Epsilonproteobacteria.Brenner, D.J., Krieg, N.R., Staley, J.T. et al., Springer, New York.

Bergey’s Manual of Systematic Bacteriology, 2009. Second edition, Volume 3, The Firmicutes. De Vos, P., Garrity, G.M., Jones, D., Krieg, N.R., Ludwig, W., Rainey, F.A., Schleifer, K.-H., Whitman, W.B. Springer, New York.

Bergey’s Manual of Systematic Bacteriology, 2012. Second edition, Volume 5, The Actinobacteria, Part A. Goodfellow, M., Kämpfer, P., Busse, H.-J., Trujillo, M.E., Suzuki, K.-I., Ludwig, W., Whitman, W.B. Springer, New York.

Beech, I.B., Gaylarde, Ch.C., 1999. Recent advances in the study of biocorrosion: an overview. Revista de Microbiologia 30 (3), 117– 190.

Bermont-Bouis, D., Janvier, M., Grimont, P.A., Dupont, I., Vallaeys, T., 2007. Both sulfate-reducing bacteria and Enterobacteriaceae take part in marine biocorrosion of carbon steel. Journal of Applied Microbiology 102, 161–168.

Bleich, R., Watrous, J.D., Dorrestein, P.C., Bowers, A.A., Shank, E.A., 2015. Thiopeptide antibiotics stimulate biofilm formation in Bacillus subtilis. Proceedings of the National Academy of Sciences (PNAS) 112 (10), 3086–3091.

Bolton, N., Critchley, M., Fabien, R., Cromar, N., Fallowfield, H., 2010. Microbially influenced corrosion of galvanized steel pipes in aerobic water systems. Journal of Applied Microbiology 109, 239–247.

Bragadeeswaran,S., Jeevapriya, R., Prabhu, K., Sophia Rani, S., Priyadharsini, S., Balasubramanian, T., 2011. Exopolysaccharide production by Bacillus cereus GU812900, a fouling marine bacterium. African Journal of Microbiology Research 5 (24), 4124–4132.

Capão, A., Moreira-Filho, P., Garcia, M., Bitati, S., Luciano Procópio, L., 2020. Marine bacterial community analysis on 316L stainless steel coupons by Illumina MiSeq sequencing. Biotechnology Letters 42, 1431–1448.

Costerton, J.W., Lewandowski, Z., Caldwell, D.E., Korber, D.R., Lappin- Scott, H.M. Microbial Biofilms, 1995. Annual Review of Microbiology 49, 711–745.

Du, J., Li, S., Liu, J., Yu, M., 2014. Corrosion behavior of steel Q235 co-influenced by Thiobacillus thiooxidans and Bacillus. Beijing Hangkong Hangtian Daxue Xuebao. Journal of Beijing University of Aeronautics and Astronautics 40 (1), 31–38.

Duque, Z., Ibars, J.R., Sarró, M.I., Moreno, D.A., 2011. Comparison of sulphide corrosivity of sulphate- and non-sulphate-reducing prokaryotes isolated from oilfield injection water. Materials and Corrosion 62 (9999), 1–7.

Engel, K., Ford, S.E., Coyotzi, S., McKelvie, J., Diomidis, N., Slater, G., Neufeld, J.D., 2019. Stability of Microbial Community Profiles Profiles Associated with Compacted Bentonite from the Grimsel Underground Research Laboratory. mSphere 4 (6) e00601-19. https://doi.org/10.1128/mSphere.00601-19

Giovannoni, S.J., Britschgi, T.B., Moyer, C.L., Field, K.G., 1990. Genetic diversity in Sargasso Sea bacterioplankton. Nature, 345, 60–63.

Herro, H.M., Port, R.D., 1993. The Nalco guide to cooling water system failure analysis, 1st ed., McGraw-Hill, New York, pp. 420.

Horn, J., Carrrillo, C., Dias, V., 2003. Comparison of the Microbial Community Composition at Yucca Mountain and Laboratory Test Nuclear Repository Environments. CORROSION ⁄2003 (San Diego, CA, March 16–20, 2003), Paper No. 03556. NACE International, Houston.

Ilhan-Sungur, Е., Ozuolmez, D., Çotuk, A., Cansever, N., Muyzer, G., 2017. Isolation of a sulfide-producing bacterial consortium from coolingtower water: Evaluation of corrosive effects on galvanized steel. Anaerobe 43, 27–34.

James, G.A., Beaudette, L., Costerton, J.W., 1995. Interspecies bacterial interactions in biofilm. The Journal of Industrial Microbiology and Biotechnology 15 (4), 237–262.

Jan-Roblero, J., Romero, J.M., Amaya, M., Le Borgne, S., 2004. Phylogenetic characterization of a corrosive consortium isolated from a sour gas pipeline. Applied Microbiology and Biotechnology 64, 862–867.

Jayaraman, A., Earthman, J.C., Wood, T.K., 1997. Corrosion inhibition by aerobic biofilms on SAE 1018 steel. Applied Microbiology and Biotechnology 47, 62–68.

Lane, D.G., 1991. Nucleic acids techniques in bacterial systematic. In: Stackebrandt, E., Goodfellow, M. (Eds), Nucleic Acid Techniques in Bacterial Systematic, John Wiley and Sons, New York, 115–175.

Lewandowski, Z., 2000. Structure and Function of Biofilms. In: Evans, L.V. (Ed.) Biofilms: Recent Advances in Their Study and Control, 1–17, Harwood Academic Publishers.

Li, X., Duan, J., Xiao, H., Li, Y., Liu, H., Guan, F., Zhai, X., 2017. Analysis of Bacterial Community Composition of Corroded Steel Immersed in Sanya and Xiamen Seawaters in China via Method of Illumina MiSeq Sequencing. Frontiers in microbiology 8, 1737.

Lopez, M.A., Serna, F.J.Z., Jan-Roblero, J., Romero, J.M., Hernandez- Rodriguez, C., 2006. Phylogenetic analysis of a biofilm bacterial population in a water pipeline in the Gulf of Mexico. FEMS Microbiology Ecology 58, 145–154.

Lovley, D.R., Phillips, E.J.P., 1988. Novel mode of microbial energy metabolism: organic carbon oxidation coupled to dissimilatory reduction of iron or manganes, Applied and Environmental Microbiology 54 (6), 1472–1480.

Magot, M., Ravot, G., Campaignolle, X., Ollivier, B., Patel, B.K., Fardeau, M.L., Thomas, P., Crolet, J.L., Garcia, J.L., 1997. Dethiosulfovibrio peptidovorans gen. Nov., sp. Nov., a new anaerobic, slightly halophilic, thiosulfate-reducing bacterium from corroding offshore oil wells. International Journal of Systematic and Evolutionary Microbiology 47, 818–824.

Methods of general bacteriology: in three volumes (Metodyi obschey mikrobiologii), 1984. Gerhardt, F. et al. (Ed.), 3, Mir, Moscow. (in Russian).

Monroy, O.A.R., Gayosso, M.J.H., Ordaz, N.R., Olivares, G.Z., Ramírez, C.J., 2011. Corrosion of API XL 52 steel in presence of Clostridium celerecrescens. Materials and Corrosion 62 (9), 878–883.

Neria-Gonzalez, I., Wang, E.T., Ramirez, F., Romero, J.M., Hernandez- Rodriguez, C., 2006. Characterization of bacterial community associated to biofilms of corroded oil pipelines from the southeast of Mexico. Anaerobe 12, 122–133.

Nnabuk Eddy Okon, 2010. Fermentation product of Streptomyces griseus (albomycin) as a green inhibitor for the corrosion of zinc in H2SO4. Green Chemistry: Letters and Reviews 3 (4), 307–314.

Nuňez, M., 2007. Prevention of metal corrosion: new research. Nova Science Publishers, Inc., New York, pp. 310.

Okabe ,S., Odagiri, M., Ito, T., Satoh, H., 2007. Succession of sulfur- oxidizing bacteria in the microbial community on corroding concrete in sewer systems. Applied and Environmental Microbiology 73, 971–980.

Oliveira, V.M., Lopes-Oliveira, P.F., Passarini, M.R.Z., Menezes, C.B.A., Oliveira, W.R.C., Rocha, A.J., Sette, L.D., 2011. Molecular analysis of microbial diversity in corrosion samples from energy transmission towers. Biofouling 27 (4), 435–447.

Pacheco da Rosa, J., Korenblum, E., Franco-Cirigliano, M.N., Abreu, F., Lins, U., Soares, R.M.A., Macrae, A., Seldin, L., Coelho, R.R.R., 2013. Streptomyces lunalinharesii Strain 235 Shows the Potential to Inhibit Bacteria Involved in Biocorrosion Processes. Hindawi Publishing Corporation BioMed Research International, Article ID 309769.

Pacheco da Rosa, J., Tiburcio, S.R.G., Marques, J.M., Seldin, L., Coelho, R.R.R., 2016. Streptomyces lunalinharesii 235 prevents the formation of a sulfate-reducing bacterial biofilm. Brazilian journal of microbiology 47, 603–609.

Pavissich, J.P., Vargas, I.T., Gonzalez, B., Pasten, P.A., Pizarro, G.E., 2010. Culture dependent and independent analyses of bacterial communities involved in copper plumbing corrosion. Journal of Applied Microbiology 109, 771–782.

Pope, D.H., Duquette, D.J., Johannes, A.H., Wayner, P.C., 1984. Microbially influenced corrosion of industrial alloys. Materials Performance 23 (4), 14–15.

Pilyashenko-Novokhatny, A.I., 2000. Possible distribution of functions between the components of corrosion-hazardous aggregates of microorganisms in the general process of microbially induced corrosion (Mozhlyvyi rozpodil funktsii mizh skladovymy koroziinonebezpechnymy sukupnostiamy mikroorhanizmiv v zahalnomu protsesi mikrobno indukovanoi koroziii). Materials IV International. Conference-exhibitions “Problems of corrosion and anticorrosion. Protection of materials” (Corrosion-200). G.V. Karpenko Physical-Mechanical Institute of the National Academy of Sciences of Ukraine, Lviv. 564–567. (in Ukrainian)

Plohinskij, N.A., 1970. Biometrics (Biometriya). Izdatel’stvo Moskovskogo universiteta, Moskva. (in Russian)

Purish, L.M., Asaulenko, L.G., 2007. Dynamics of succession changes in sulfidogenic microbial association under conditions of biofilm formation on the surface of steel. Mikrobiologii Zhurnal 69 (6), 19‑25. (in Ukrainian with English summary)

Purish, L.M., Asaulenko, L.G., Ostapchuk, A.M., 2009. Features of development of mono- and associative cultures of sulfate-reducing bacteria and formation of exopolymer complex. Mikrobiologii Zhurnal 71 (2), 20–26. (in Ukrainian with English summary)

Qiu, Y.-Y., Guo, J.-H., Zhang, L., Chen, G.-H., Jiang, F., 2017. A highrate sulfidogenic process based on elemental sulfur reduction: cost-effectiveness evaluation and microbial community analysis. Biochemical Engineering Journal 128, 26–32.

Rajasekar, A., Ting, Y.-P., 2010. Microbial Corrosion of Aluminum 2024 Aeronautical Alloy by Hydrocarbon Degrading Bacteria Bacillus cereus ACE4 and Serratia marcescens ACE2. Industrial & Engineering Chemistry Research 49, 6054–6061.

Romanenko, V.I., Kuznetsov, S.I., 1974. Ecology of microorganisms of fresh reservoirs (Ekologiya mikroorganizmov presnyih vodoemov), Nauka, Leningrad. (in Russian).

Salgar-Chaparro, S.J., Darwin, A., Kaksonen, A.H., Machuca, L.L., 2020. Carbon steel corrosion by bacteria from failed seal rings at an offshore facility. Scientific reports 10 (1), 12287. https://doi.org/10.1038/s41598-020-69292-5

Salgar-Chaparro, S.J., Silva-Plata, B.A., 2008. Caracterizacion de la comunidad microbiana residente en aguas de produccion de tres campos de explotacion petrolera, con especial enfasis en grupos asociados a procesos corrosivos. Proyecto. Universidad Industrial de Santander. (in Spanish with English summary)

Satoh, H., Odagiri, M., Ito, T., Okabe, S., 2009. Microbial community structures and in situ sulfate-reducing and sulfur-oxidizing activities in biofilms developed on mortor specimens in a corroded sewer system. Water Research 43, 4729–4739.

Stahl, D.A., Lane, D.I., Olsen, G.L., Pace, N.R., 1984. Analysis of hydrothermal vent-associated symbionts by ribosomal RNA sequences. Science, 224, 409–411.

Su, H., Mi, Sh., Peng, X., Han, Y., 2019. The mutual influence between corrosion and the surrounding soil microbial communities of buried petroleum pipelines. RSC Advances 9, 18930–18940.

Tamura, K., Stecher, G., Peterson, D., Filipski, A., Kumar, S., 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30 (12), 2725–2729.

Tkachuk, N., Zelena, L., Mazur, P., Lukash, O., 2020. Genotypic, physiological and biochemical features of Desulfovibrio strains in a sulfidogenic microbial community isolated from the soil of ferrosphere. Ecological questions 31 (2), 79–88.

Tkachuk, N.V., Zelena, L.B., Parmynska, V.S., Yanchenko, V.O., Demchenko, A.M., 2017. Identification of heterotrophic soil ferrosphere bacteria and their sensitivity to the pesticide linuron, Mikrobiologii Zhurnal 9 (4), 75–87. (in Ukrainian with English summary).

Vaschenko, I.M., Lange, K.P., Merkulov, M.P., 1982. Workshop on the basics of rural farming (Praktikum po osnovam selskogo hazyaystva), Prosveschenie, Moskva. (in Russian).

Vincke, E., Boon, N., Verstraete, W., 2001. Analysis of the microbial communities on corroded concrete sewer pipes – a case. Applied Microbiology and Biotechnology 57, 776–785.

Wang, Y.S., Liu, L., Fu, Q., Sun, J., An, Z.Y., Ding, R., Li, Y., Zhao, X.D., 2020. Effect of Bacillus subtilis on corrosion behavior of 10MnNiCrCu steel in marine environment. Scientific Reports 10, 5744. http://dx.doi.org/10.1038/s41598-020-62809-y

Zhu, X., Lubeck, J., Kilbane II J.J., 2003. Characterization of microbial communities in gas industry pipelines. Applied and Environmental Microbiology 69, 5354–5363.
Go to article

Authors and Affiliations

Nataliia Tkachuk
1
Liubov Zelena
2

  1. Department of Biology, T.H. Shevchenko National University “Chernihiv Colehium”, Hetman Polubotok Str. 53, 14013, Chernihiv, Ukraine
  2. Department of Physiology of Industrial Microorganisms, Danylo Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Acad. Zabolotny Str. 154, 03143 Kyiv, Ukraine
Download PDF Download RIS Download Bibtex

Abstract

Filtration through biologically active carbon (BAC) filters is an effective method of organic matter removal during drinking water treatment. In this study, the microbial community in the initial period of filters’ operation, as well as its role in the organic matter removal were investigated. Research was carried out in a pilot scale on two BAC filters (Filter 1 and Filter 2) which were distinguished by the type of inflowing water. It was observed that the number of heterotrophic plate count bacteria and total microbial activity were significantly higher in water samples collected from Filter 2, which received an additional load of organic matter and microorganisms. Despite the differences in the values of chemical and microbiological parameters of inflowing water, the composition of the microbiome in both filters was similar. The predominant taxon was a bacterium related to Spongiibacter sp. (Gammaproteobacteria) (>50% of relative abundance). In both filters, the efficiency of organic matter removal was at the same level, and the composition and relative frequency of predicted functional pathways related to metabolism determined using PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States Software) at level 3 of KEGG (Kyoto Encyclopedia of Genes and Genomes) Orthology – were also similar. The study demonstrated that a 40-day period of filter operation after filling with virgin granular activated carbon, was sufficient to initiate biofilm development. It was proved, that during the initial stage of filter operation, microorganisms capable of biodegradation of various organic compounds, including xenobiotics like nitrotoluene, colonized the filters
Go to article

Bibliography

  1. APHA (2017). Standard Methods for the Examination of Water and Wastewater, (23st ed.) American Public Health Association, Washington DC.
  2. Chan, S., Pullerits, K., Keucken, A., Persson, K.M., Paul, C.J. & Rådström, P. (2019). Bacterial release from pipe biofilm in a full-scale drinking water distribution system, NPJ Biofilms Microbiomes, 5, 9. DOI:10.1038/s41522-019-0082-9
  3. Choi, Y.C., Li, X., Raskin, L. & Morgenroth, E. (2008). Chemisorption of oxygen onto activated carbon can enhance the stability of biological perchlorate reduction in fixed bed biofilm reactors, Water Research, 42, pp. 3425–3434. DOI:10.1016/j.watres.2008.05.004
  4. Dong, S., Liu, L., Zhang, Y. & Jiang, F. (2019). Occurrence and succession of bacterial community in O3/BAC process of drinking water treatment, International Journal of Environmental Research and Public Health, 16, 3112. DOI:10.3390/ijerph16173112
  5. Douglas, G.M., Maffei, V.J., Zaneveld, J.R., Yurgel, S.N., Brown, J.R., Taylor, C.M., Huttenhower, C. & Langille, M.G.I. (2020). PICRUSt2 for prediction of metagenome functions, Nature Biotechnology, 38, pp. 685–688. DOI:10.1038/s41587-020-0548-6
  6. Edgar, R.C. (2013). UPARSE: highly accurate OTU sequences from microbial amplicon reads, Nature Methods, 10, pp. 996–998. DOI:10.1038/nmeth.2604
  7. Garrity, G.M. (Ed.) 2005. Bergey’s Manual of Systematic Bacteriology. Vol. 2 The Proteobacteria, part C, The Alpha- Beta-, Delta- and Epsilonproteobacteria, Springer, New York, pp. 1-1388. DOI:10.1007/0-387-29298-5
  8. Guo, X., Xie, C., Wang, L., Li, Q. & Wang, Y. (2019). Biodegradation of persistent environmental pollutants by Arthrobacter sp., Environmental Science and Pollution Research, 26, pp. 8429–8443. DOI:10.1007/s11356-019-04358-0
  9. Hayward, C., Ross, K.E., Brown, M.H., Bentham, R. & Whiley, H. (2022) The presence of opportunistic premise plumbing pathogens in residential buildings: a literature review, Water, 14, 1129. DOI:10.3390/w14071129
  10. Heberle, H., Meirelles, G.V., da Silva, F.R., Telles, G.P. & Minghim, R. (2015). InteractiVenn: a web-based tool for the analysis of sets through Venn diagrams, BMC Bioinformatics, 16, 169. DOI:10.1186/s12859-015-0611-3
  11. Holc, D., Pruss, A., Michałkiewicz, M. & Cybulski, Z. (2016). Effectiveness of Organic Compounds Removing During Water Treatment by Filtration Through a Biologically Active Carbon Filter with the Identification of Microorganisms, Annual Set The Environment Protection, 18, pp. 235–246 (in Polish), available on: http://ros.edu.pl/images/roczniki/2016/No2/17_ROS_N2_V18_R2016.pdf
  12. Holc, D., Mądrecka-Witkowska, B., Komorowska-Kaufman, M., Szeląg-Wasielewska, E., Pruss, A. & Cybulski, Z. (2021). The application of different methods for microbial development assessment in pilot scale drinking water biofilters, Archives of Environmental Protection, 47, 3, pp. 37-49. DOI:10.24425/aep.2021.138462
  13. Holc, D., Pruss, A., Komorowska-Kaufman, M., Mądrecka, B. & Cybulski, Z. (2019). The sorption of organic compounds from water during technological start-up of carbon filters, E3S Web Conferences, 100, 00027. DOI:10.1051/e3sconf/201910000027
  14. IARC, Monographs on the Evaluation of Carcinogenic Risks to Humans. (2012). Some chemicals present in industrial and consumer products, Food And Drinking-Water, 101, 9-549.
  15. Jean, W.D., Yeh, Y.T., Huang, S.P., Chen, J.S. & Shieh, W.Y. (2016). Spongiibacter taiwanensis sp. nov., a marine bacterium isolated from aged seawater, International Journal of Systematic and Evolutionary Microbiology, 66, pp. 4094–4098. DOI:10.1099/ijsem.0.001316
  16. Jin, L., Ko, S.R., Ahn, C.Y., Lee, H.G. & Oh, H.M. (2016). Rhizobacter profundi sp. nov., isolated from freshwater sediment, International Journal of Systematic and Evolutionary Microbiology, 66, pp. 1926-1931. DOI:10.1099/ijsem.0.000962
  17. Kaarela, O.E., Harkki, H.A., Palmroth, M.R.T. & Tuhkanen, T.A. (2015). Bacterial diversity and active biomass in full-scale granular activated carbon filters operated at low water temperatures, Environmental Technology, 36, pp. 681-692. DOI:10.1080/09593330.2014.958542
  18. Kanehisa, M., Furumichi, M., Sato, Y., Ishiguro-Watanabe, M. & Tanabe, M. (2021). KEGG: Integrating viruses and cellular organisms, Nucleic Acids Research, 49, D545–D551. DOI:10.1093/nar/gkaa970
  19. Kennedy, A.M., Reinert, A.M., Knappe, D.R.U., Ferrer, I. & Summers R.S. (2015). Full- and pilot-scale GAC adsorption of organic micropollutants, Water Research, 68, pp. 238-248. DOI:10.1016/j.watres.2014.10.010
  20. Khan M.F., Jamal A., Rosy P. J., Alguno A.C., Ismail M., Khan I., Ismail, A. & Zahid, M. (2022). Eco-friendly elimination of organic pollutants from water using graphene oxide assimilated magnetic nanoparticles adsorbent, Inorganic Chemistry Communications, 139, 109422. DOI:10.1016/j.inoche.2022.109422
  21. Korotta-Gamage, S.M. & Sathasivan, A. (2017). A review: Potential and challenges of biologically activated carbon to remove natural organic matter in drinking water purification process, Chemosphere, 167, pp. 120-138. DOI:10.1016/j.chemosphere.2016.09.097
  22. Langille, M.G.I., Zaneveld, J., Caporaso, J.G., McDonald, D., Knights, D., Reyes, J.A., Clemente, J.C., Burkepile, D.E., Vega Thurber, R.L., Knight, R., Beiko, R.G. & Huttenhower, C. (2013). Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences, Nature Biotechnology, 31, pp. 814–821. DOI:10.1038/nbt.2676
  23. LaPara, T.M., Hope Wilkinson, K., Strait, J.M., Hozalski, R.M., Sadowksy, M.J &, Hamilton, M.J. (2015). The Bacterial Communities of Full-Scale Biologically Active, Granular Activated Carbon Filters Are Stable and Diverse and Potentially Contain Novel Ammonia-Oxidizing Microorganisms, Applied and Environmental Microbiology, 81, pp. 6864-6872. DOI:10.1128/AEM.01692-15
  24. Li, C., Ling, F., Zhang, M., Liu, W.T., Li, Y. & Liu, W. (2017). Characterization of bacterial community dynamics in a full-scale drinking water treatment plant, Journal of Environmental Sciences, 51, pp. 21-30. DOI:10.1016/j.jes.2016.05.042
  25. Liao, X., Chen, C., Chang, C.-H., Wang, Z., Zhang, X. & Xie, S. (2012). Heterogeneity of microbial community structures inside the up-flow biological activated carbon (BAC) filters for the treatment of drinking water. Biotechnology and Bioprocess Engineering, 17, pp. 881–886. DOI:10.1007/s12257-012-0127-x
  26. Liao, X., Chen, C., Wang, Z., Chang, C.-H., Zhang, X. & Xie, S. (2015). Bacterial community change through drinking water treatment processes, International Journal of Environmental Science and Technology, 12, pp. 1867-1874. DOI:10.1007/s13762-014-0540-0
  27. Liao, X., Chen, C., Wang, Z., Wan, R., Chang, C.-H. & Zhang, X. (2013). Changes of biomass and bacterial communities in biological activated carbon filters for drinking water treatment. Process Biochemistry, 48, pp. 312-316. DOI:10.1016/j.procbio.2012.12.016
  28. Liu, G., Zhang, Y., van der Mark, E., Magic-Knezev, A., Pinto, A., van den Bogert, B., Liu, W., van der Meer, W. & Medema, G. (2018). Assessing the origin of bacteria in tap water and distribution system in an unchlorinated drinking water system by SourceTracker using microbial community fingerprints, Water Research, 138, pp. 86-96. DOI:10.1016/j.watres.2018.03.043
  29. Ma, B., LaPara, T.M. & Hozalski, R.M. (2020). Microbiome of Drinking Water Biofilters is Influenced by Environmental Factors and Engineering Decisions but has Little Influence on the Microbiome of the Filtrate, Environmental Science & Technology, 54, pp. 11526-11535. DOI:10.1021/acs.est.0c01730
  30. Makowska, N., Philips, A., Dabert, A., Nowis, K., Trzebny, A., Koczura, R. & Mokracka, J. (2020). Metagenomic analysis of β-lactamase and carbapenemase genes in the wastewater resistome, Water Research, 170, 115277. DOI:10.1016/j.watres.2019.115277
  31. Matilainen, A., Vieno N., & Tuhkanen, T. (2006). Efficiency of the activated carbon filtration in the natural organic matter removal, Environment International, 32, pp. 324-331. DOI:10.1016/j.envint.2005.06.003
  32. Mądrecka, B., Komorowska-Kaufman, M., Pruss, A. & Holc, D. (2018). Metabolic activity tests in organic matter biodegradation studies in biologically active carbon filter beds, in: Water Supply and Wastewater Disposal, Sobczuk, H. & Kowalska, B. (Eds.), Lublin University of Technology, Lublin, pp.163-177
  33. Magic-Knezev, A., Wullings, B. & Van der Kooij, D. (2009). Polaromonas and Hydrogenophaga species are the predominant bacteria cultured from granular activated carbon filters in water treatment, Journal of Applied Microbiology, 107, pp. 1457-1467. DOI:10.1111/j.1365-2672.2009.04337.x
  34. Matsis, V. M. & Grigoropoulou, H.P. (2008). Kinetics and equilibrium of dissolved oxygen adsorption on activated carbon, Chemical Engineering Science, 63, pp. 609-621. DOI:10.1016/j.ces.2007.10.005
  35. Oh, S., Hammes, F. & Liu, W.T. (2018). Metagenomic characterization of biofilter microbial communities in a full-scale drinking water treatment plant, Water Research, 128, pp. 278-285. DOI:10.1016/j.watres.2017.10.054
  36. Papciak, D., Kaleta, J., Puszkarewicz, A. & Tchorzewska-Cieślak, B. (2016). The use of biofiltration process to remove organic matter from groundwater, Journal of Ecological Engineering, 17, pp. 119-124. DOI:10.12911/22998993/63481
  37. PN-C-04578-02:1985 Water and wastewater - Testing of oxygen demand and organic carbon content - Determination of chemical oxygen demand (COD) by the permanganate method. (in Polish)
  38. Qi, W., Li, W., Zhang, J. & Zhang, W. (2019). Effect of biological activated carbon filter depth and backwashing process on transformation of biofilm community, Frontiers of Environmental Science & Engineering, 13, 15. DOI:10.1007/s11783-019-1100-0
  39. Rosenberg, E., DeLong E.F., Lory, S., Stackebrandt, E., Thompson, F. (Eds.), (2014). The Prokaryotes. Alphaproteobacteria and Betaproteobacteria. (4rd ed.), Springer, Berlin, Heidelberg. pp. 3-1012. DOI:10.1007/978-3-642-30197-1
  40. Dos Santos, P.R. & Daniel, L.A. (2020). A review: organic matter and ammonia removal by biological activated carbon filtration for water and wastewater treatment, International Journal of Environmental Science and Technology, 17, pp. 591-606. DOI:10.1007/s13762-019-02567-1
  41. Selbes, M., Brown, J., Lauderdale, C. & Karanfil, T. (2017). Removal of Selected C‐ and N‐DBP Precursors in Biologically Active Filters, Journal ‐ American Water Works Association, 109: E73-E84. DOI:10.5942/jawwa.2017.109.0014
  42. Servais, P., Billen, G. & Bouillot, P. (1994). Biological colonization of granular activated carbon filters in drinking-water treatment, Journal of Environmental Engineering, 120, 4, pp. 888-899. DOI:10.1061/(ASCE)0733-9372(1994)120:4(888)
  43. Shirey, T.B., Thacker, R.W. & Olson, J.B. (2012). Composition and stability of bacterial communities associated with granular activated carbon and anthracite filters in a pilot scale municipal drinking water treatment facility, Journal of Water and Health, 10, pp. 244–255. DOI:10.2166/wh.2012.092
  44. Simpson, D.R. (2008). Biofilm processes in biologically active carbon water purification, Water Research, 42, pp. 2839-2848. DOI:10.1016/j.watres.2008.02.025
  45. Su, H.-C., Liu, Y.-S., Pan C.-G., Chen, J., He, L.-Y. & Ying, G.-G. (2018). Persistence of antibiotic resistance genes and bacterial community changes in drinking water treatment system: From drinking water source to tap water, Science of the Total Environment, 616–617, pp. 453-461. DOI:10.1016/j.scitotenv.2017.10.318
  46. Velten, S., Boller, M., Köster, O., Helbing, J., Weilenmann, H.U. & Hammes, F. (2011). Development of biomass in a drinking water granular active carbon (GAC) filter, Water Research 45, pp. 6347-6354. DOI:10.1016/j.watres.2011.09.017
  47. Vignola, M., Werner,D., Wade, M.J., Meynet, P. & Davenport, R.J. (2018). Medium shapes the microbial community of water filters with implications for effluent quality, Water Research, 129, pp. 499-508. DOI:10.1016/j.watres.2017.09.042.
  48. Waak, M.B., Hozalski, R.M., Hallé, C. & LaPara, T.M. (2019). Comparison of the microbiomes of two drinking water distribution systems - with and without residual chloramine disinfection, Microbiome, 7, 87. DOI:10.1186/s40168-019-0707-5
  49. White, C.P., Debry, R.W. & Lytle, D.A. (2012). Microbial survey of a full-scale, biologically active filter for treatment of drinking water, Applied and Environmental Microbiology, 78, pp. 6390-6394. DOI:10.1128/AEM.00308-12
  50. Yapsakli, K. & Çeçen, F. (2010). Effect of type of granular activated carbon on DOC biodegradation in biological activated carbon filters, Process Biochemistry, 45, pp. 355-362. DOI:10.1016/j.procbio.2009.10.005
Go to article

Authors and Affiliations

Beata Mądrecka-Witkowska
1
ORCID: ORCID
Małgorzata Komorowska-Kaufman
1
ORCID: ORCID
Alina Pruss
1
ORCID: ORCID
Dorota Holc
1
ORCID: ORCID
Artur Trzebny
2
ORCID: ORCID
Miroslawa Dabert
2
ORCID: ORCID

  1. Poznan University of Technology, Institute of Environmental Engineering and Building Installations, Poznań, Poland
  2. Adam Mickiewicz University in Poznań, Faculty of Biology, Poznań, Poland
Download PDF Download RIS Download Bibtex

Abstract

Strawberry leaves showing leaf blight symptoms were collected from six different farms in Ismailia and Beheira Governorates in Egypt during the 2020–2021 growing season. Eight bacterial isolates, i.e., Pa1, Pa2, Pa3, Pa4 (Ismailia farms) and Pa5, Pa6, Pa7 and Pa8 (Beheira farms) were isolated. A pathogenicity test of bacterial isolates was carried out using detached strawberry leaf technique. All bacterial isolates produced leaf blight disease symptoms. Isolates Pa2 and Pa6 showed the highest pathogenic characteristics with clear symptoms on detached strawberry leaves. The phenotypic, biochemical and physiological characters of the highest pathogenic isolates were confirmed by PCR analysis using 16S rRNA gene. The two bacterial isolates were identified as Pantoea ananatis with similarity of 97.05% with accession number MH_127816.1 (isolate Pa2, Ismailia), while the isolate ( Pa6, Beheira) with similarity of 97.03% with accession number NR_026045.1. The 16S rDNA sequences were deposited in the GenBank nucleotide databases under accession numbers OM258167 and OM279507, respectively. According to the pathogenicity test, morphological and physiological characteristics as well as molecular data (16S rRNA sequencing analysis), this finding is the first report of P. ananatis as a causal agent of strawberry leaf blight disease in Egypt.
Go to article

Authors and Affiliations

Tarek Gomaa Abdel-Gaied
1
ORCID: ORCID
Hassan Abd-El-Khair
1
ORCID: ORCID
Maryan Makram Youssef
2
Shereen Abu El-Maaty
3
Maurice Sabry Mikhail
2

  1. Department of Plant Pathology, National Research Centre, Dokki, Giza, Egypt
  2. Department of Plant Pathology, Faculty of Agriculture, Cairo University, Giza, Egypt
  3. Department of Genetics, Faculty of Agriculture, Cairo University, Giza, Egypt
Download PDF Download RIS Download Bibtex

Abstract

Here we investigate the microbiomes of the soil samples from the Yamal Peninsula (the surroundings of Salekhard city, Russian Federation) using a high-throughput sequencing approach. The main goal was to investigate the impact of mining on soils within the following regeneration, both during the reclamation practice and natural self-growth. Several quarries were studied, engaged in sand, clay and chromatic ores mining. The taxonomic analysis of the soil microbiomes revealed 50 bacterial and archaeal phyla; among the dominant phyla were: Proteobacteria, Actinobacteria, Acidobacteria, Chroloflexi, Gemmatimonadetes, Verrucomicrobia, Planctomycetes, Bacteroidetes, AD3, and Nitrospirae. Compared to the typical tundra soil, which was chosen as a control, the disturbed soils had increased biodiversity and total counts for soil bacteria, archaea, and fungi, especially in the cryosolic horizon. The different mining strategies caused significantly different transformations of soil microbiomes, which was less pronounced for self-growth compared to reclaimed quarries. This isolation of the reclaimed quarry was mainly associated with the increase of the amount of acidobacteria (fam. Koribacteraceae and Acidobacteriaceae and order Ellin6513), some proteobacterial taxa (fam. Syntrophobacteraceae), and Chloroflexi (fam. Thermogemmatisporaceae). The study also revealed bacteria, which tend to be specific for marine tundra environments: gemmatimonadetes from the order N1423WL and Chloroflexi bacteria from the order Gitt-GS-136.

Go to article

Authors and Affiliations

Elizaveta Pershina
Ekaterina Ivanova
Anastasia Kimeklis
Alexey Zverev
Arina Kichko
Tatiana Aksenova
Evgeny Andronov
Evgeny Abakumov
ORCID: ORCID
Download PDF Download RIS Download Bibtex

Abstract

Ammonia-oxidizing bacteria communities were evaluated in a completely mixed, laboratory scale membrane reactor (MBR) working under anoxic conditions for 5 months. The microorganisms in activated sludge were fed a synthetic medium containing 66-150 mg NH4 +-N/l. The age of the activated sludge in MBR was 50 days and the hydraulic retention time (HRT) was 3.3 days. The estimation of the diversity and complexity of the AOB community together with the identification of the dominant bacteria in the activated sludge under anoxic conditions were performed using denaturing gradient gel electrophoresis (DGGE) and DNA sequencing. Molecular analysis of the microbial community carried out with two microbial molecular markers, 16S rRNA gene and amoA gene, suggested that nitrification was led by a Nitrosomonas-like species. In the biocenosis of the investigated bioreactor, oxygen was the crucial selective parameter. The results obtained in this work showed that amoA gene research is more suitable to study the stability and effectiveness of ammonia oxidation. This information emphasizes the necessity of the usage of molecular markers based on functional genes instead of ribosomal ones in order to present the actual state of the process performed in bioreactors. It was also stated that Nitrosomonas -like bacteria are able to perform nitritation even in anoxic environment, that is probably the reason why these bacteria are the most common AOB in different bioreactors.

Go to article

Authors and Affiliations

Aleksandra Ziembińska
Sławomir Ciesielski
Anna Raszka
Korneliusz Miksch

This page uses 'cookies'. Learn more