The systematic position of Sorbus population occurring in the Pieniny Mts. is controversial. To verify its taxonomic status we studied the ITS sequence of closely related species of the S. aria group: Sorbus sp. from the Pieniny Mts., S. aria from the Tatra Mts., S. graeca from the Balkans, and other well-distinguished native Polish Sorbus species (S. aria, S. aucuparia, S. intermedia and S. torminalis). As a reference we examined Sorbus populations closest to the Pieniny Mts. where S. graeca was reported to occur, in Slovakia. The results indicate that the Sorbus plants found in the Pieniny Mts. differ genetically from those in the Tatra Mts. but are identical to those collected from the Vihorlat Mts. in Slovakia and are closely related to S. graeca from the Balkans
This work was carried out during two successive seasons (2016 and 2017) on cucumber fruits from a plastic greenhouse and from open field cultivation in El Gharbeia and El Giza Governorates, Egypt. Isolation trials from spoilage fruit samples of plastic greenhouse cultivation recorded high frequency of Alternaria tenusinium, Fusarium spp. and Pleospora alli. The most common fungi of rotten cucumber fruits from an open field were Galactomyces spp. and Fusarium spp. Pathogenicity tests proved that, Fusarium solani from El-Gharbeia followed by A. tenusinium from El-Giza were the most frequent isolates responsible for rot of cucumber fruits from plastic greenhouse cultivation. Moreover, the most frequent isolates causing postharvest disease of cucumber fruits of the open field were Galactomyces candidium from El-Giza followed by Geotrichum sp. and F. fujikuroi from El-Gharbeia Governorates, respectively. This is the first report of several fungi causing postharvest fruit rot disease of cucumber i.e., G. candidium, Geotrichum sp., A. tenusinium, P. alli and Fusarium spp. (F. fujikuroi, F. verticiolides, F. solani, F. geraminearium and Fusarium incarnatum). Fungal isolates were identified according to cultural, morphological and molecular characterization based on sequencing of internal transcribed spacer1 (ITS1). All the ITS nucleotide sequences of fungi were applied and conserved in GenBank.
Kiwifruit (Actinidia deliciosa) is one of the most significant commercial crops in Iran. In 2015 a destructive disease of kiwifruits was observed in orchards, storage facilities and retail markets, resulting in great economic loss to producers. In this study phenotypic and molecular techniques were applied to characterize the causal agent of kiwifruit rot observed in Mazandaran province, northern Iran. From the similarity among the results of pathogenicity tests, equivalency with standard taxonomic criteria for disease and PCR-based analysis of the ITS region, all the isolates were identified as Botryosphaeria dothidea.
Until recently, Festuca arietina was practically an unknown species in the flora of Eastern Europe. Such a situation can be treated as a consequence of insufficient studying of Festuca valesiaca group species in Eastern Europe and misinterpretation of the volume of some taxa. As a result of a complex study of F arietina populations from the territory of Ukraine (including the material from locus classicus), Belarus and Lithuania, original anatomy, morphology and molecular data were obtained. These data confirmed the taxonomical status of F arietina as a separate species. Eleven morphological and 12 anatomical characters, ITS1-5.8S-ITS2 cluster of nuclear ribosomal genes, as well as the models of secondary structure of ITS1 and ITS2 transcripts were studied in this approach. It was found for the first time that F arietina is hexaploid (6x = 42), which is distinguished from all the other narrow-leaved fescues by specific leaf anatomy as well as in ITS1-5.8S-ITS2 sequences. Molecular data indicating possible hybridogenous origin of F arietina, fall in line with the anatomical-morphological data and explain the tendency toward sclerenchyma strands fusion with formation of a continuous ring in F arietina, as well as E arietina ecological confinement to psammophyte biotopes.
The problem of improving the voltage profile and reducing power loss in electrical networks must be solved in an optimal manner. This paper deals with comparative study of Genetic Algorithm (GA) and Differential Evolution (DE) based algorithm for the optimal allocation of multiple FACTS (Flexible AC Transmission System) devices in an interconnected power system for the economic operation as well as to enhance loadability of lines. Proper placement of FACTS devices like Static VAr Compensator (SVC), Thyristor Controlled Switched Capacitor (TCSC) and controlling reactive generations of the generators and transformer tap settings simultaneously improves the system performance greatly using the proposed approach. These GA & DE based methods are applied on standard IEEE 30 bus system. The system is reactively loaded starting from base to 200% of base load. FACTS devices are installed in the different locations of the power system and system performance is observed with and without FACTS devices. First, the locations, where the FACTS devices to be placed is determined by calculating active and reactive power flows in the lines. GA and DE based algorithm is then applied to find the amount of magnitudes of the FACTS devices. Finally the comparison between these two techniques for the placement of FACTS devices are presented.
The paper deals with the new method of automatic vehicle classification called ALT (ALTernative). Its characteristic feature is versatility resulting from its open structure, moreover a user can adjust the number of vehicles and their category according to individual requirements. It uses an algorithm for automatic vehicle recognition employing data fusion methods and fuzzy sets. High effectiveness of classification while retaining high selectivity of division was proved by test results. The effectiveness of classification of all vehicles at the level of 95% and goods trucks of 100% is more than satisfactory.
This paper is both a review and a study. It discusses the taxonomic status of Yellow Archangel (Galeobdolon luteum Huds.) from historical and contemporary perspectives, and gives a comprehensive list of synonyms for the discussed genera, species and lower taxonomic units, including their publication details. In the study it is postulated that G. luteum should be included in the genus Lamium. The hypothesis is verified by a comparative analysis between the representatives of the genera Galeobdolon and Lamium in four DNA regions: ITS, accD, rpoC1 and trnH-psbA. The analysis supported the determination of phylogenetic relationships among the studied taxa: G. luteum is not genetically distant enough from Lamium to be considered a separate genus, and integration of Galeobdolon and Lamium is legitimate.