22 representative antibiotics, including 8 quinolones (QNs), 9 sulfonamides (SAs), and 5 macrolides (MCs) were selected to investigate their occurrence and removal efficiencies in a Wastewater Treatment Plant (WWTP) and their distribution in the receiving water of the Chaobai River in Beijing, China. Water quality monitoring was performed in an integrated way at different selected points in the WWTP to explore the potential mechanism of antibiotics removal during wastewater treatment. Water quality of the Chaobai River was also analyzed to examine environmental distribution in a river ecosystem. The results showed that within all the 22 compounds examined, 10 antibiotics were quantified in wastewater influent, 10 in effluent, and 7 in river. Sulfadiazine (SDZ, 396 ng/L) and Sulfamethazine (SMZ, 382 ng/L) were the dominating antibiotics in the influent. Both the conventional treatment and advanced Biological Aerated Filter (BAF) system was important for the removal of antibiotics from the wastewater. And the concentrations of selected antibiotics were ranged from 0-41.8 ng/L in the effluent-receiving river. Despite the fact that the concentrations were reduced more than 50% compared to effluent concentrations, WWTP discharge was still regarded as a dominant point-source input of antibiotics into the Chaobai River.
Antibiotics are a group of substances potentially harmful to the environment. They can play a role in bacterial resistance transfer among pathogenic and non-pathogenic bacteria. In this experiment three representatives of medically important chemotherapeutics, confirmed to be present in high concentrations in wastewater treatment plants with HPLC analysis were used: erythromycin, sulfamethoxazole and trimethoprim. Erythromycin concentration in activated sludge was not higher than 20 ng L−1. N-acetylo-sulfamethoxazole concentration was 3349 ± 719 in winter and 2933 ± 429 ng L−1 in summer. Trimethoprim was present in wastewater at concentrations 400 ± 22 and 364 ± 60 ng L−1, respectively in winter and summer. Due to a wide variety of PCR-detectable resistance mechanisms towards these substances, the most common found in literature was chosen. For erythromycin: erm and mef genes, for sulfamethoxazole: sul1, sul2, sul3 genes, in the case of trimethoprim resistance dhfrA1 and dhfr14 were used in this study. The presence of resistance genes were analyzed in pure strains isolated from activated sludge and in the activated sludge sample itself. The research revealed that the value of minimal inhibitory concentration (MIC) did not correspond with the expected presence of more than one resistance mechanisms. Most of the isolates possessed only one of the genes responsible for a particular chemotherapeutic resistance. It was confirmed that it is possible to monitor the presence of resistance genes directly in activated sludge using PCR. Due to the limited isolates number used in the experiment these results should be regarded as preliminary.
A new method for measurement of sludge blanket height (SBH) based on image analysis is presented. The proposed method uses a histogram back-projection algorithm to distinguish between the settling sludge and supernatant and can be used with sludge possessing different coloring characteristics both in the sludge color and the color of supernatant produced. Individual pixels in the acquired image are compared with a histogram of a representative sludge region. Therefore, the proposed method relies neither on the assumed shape of light intensity profile nor on the dominant sludge or supernatant color. Batch sedimentation tests are presented for different initial sludge concentrations and different background colors to simulate different sludge characteristics. Parameters of a settling velocity function are estimated based on the obtained results. Additionally, an algorithm is proposed that enables the zone settling velocity (ZSV) to be estimated before the batch sedimentation test is completed.
This work aims to evaluate the treated wastewater from the activated sludge treatment plant in the City of Sidi Bel Ab-bes (North-Western Algeria) which is required for reuse in irrigation. The control of irrigated areas downstream is done based on a pedological study. Physico-chemical analysis such as (pH, BOD5, COD and SS) indicate results in Algerian and international standards required by the WHO. The Sodium Adsorption Ratio and Electrical Conductivity values of the treated wastewater belong to the C3-S1 class. The treated wastewater has a fairly good microbiological quality that meets Algerian standards. The helminth eggs are practically absent. The concentrations of heavy metals are much lower than the limits prescribed in the Algerian decrees. Therefore, the overall processing plant efficiency is satisfactory and has the char-acteristics of a good treated water quality for reuse in the field of irrigation while protecting the environment. The pedolo-gical study of the soil samples shows that the most dominant fraction is undeveloped calcimagnetic. The planned irrigation plain covers an area of about two thousand hectares. Depending on the crops to irrigate; the development and nature of the necessary or recommended improvements, the proposed irrigation perimeter could be classified into five categories in which only three categories are irrigable. Water projects have been proposed to ensure the irrigation of three subdivided sectors.