Abstract
The aim of the present study was to investigate whether the anterior chamber constitutes part
of the normal migratory pathway of CD4+ and CD8+ lymphocytes in cattle and swine. The cells
obtained from aqueous humor of cows and pigs were stained for CD4 and CD8 receptors, and
subsequently analyzed with flow cytometry. The mean percentage of CD4+CD8-, CD4-CD8+
and CD4+CD8+ cells within the total lymphocyte population of the bovine anterior chamber was,
respectively, 17.88, 12.64 and 27.26%. In turn, the mean values of these parameters in pigs were
1.77, 38.48 and 17.45, respectively. Among bovine and porcine CD4+CD8+ cells prevalent
were those displaying CD4lowCD8low and CD4lowCD8high phenotypes, respectively. The results suggest
that the anterior chamber in cattle and swine is an element in the normal migratory pathway
of CD4+, CD8+ and CD4+CD8+ cells. Furthermore, the contribution of these subsets in the anterior
chamber lymphocyte population can differ considerably between animal species.
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