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Abstract

Most antiseptic agents are intended for use on intact skin, e.g. for hand hygiene or skin preparation before any medical procedure. This paper presents multiple emulsion-based antiseptic agents as formulations for application to body surfaces with modified release rates.
Multiple emulsions with a co-encapsulated antiseptic (phenyl salicylate – salol) and an agent preventing microorganism growth (benzoic acid) were formed in a Couette–Taylor flow apparatus. Results confirmed the possibility of the release kinetics modification while two compounds were encapsulated in the internal droplets of emulsions to control the release rates and time of the dose release. The addition of benzoic acid as a second active compound of the encapsulation process in the internal phase of double O1/W/O2 emulsion reduced the time necessary for the total release of salol triggering a two-step release.
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Bibliography

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Authors and Affiliations

Agnieszka Markowska-Radomska
1
ORCID: ORCID
Ewa Dluska
1
ORCID: ORCID
Agata Metera
1
Maria Wojcieszak
1

  1. Warsaw University of Technology, Faculty of Chemical and Process Engineering, Warynskiego 1, 00-645 Warsaw, Poland
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Abstract

Antiseptic agents used in the postoperative period affect the functions of many tissues in the body, including the testicles. In this study, the effect of dressings administered with different antiseptic agents on testicular functions in rats that underwent abdominal incisions was investigated. A total of 48 Sprague-Dawley rats were used in the study. Each of the rats in the study group underwent a 4 cm-long skin and muscle operation. The incision was then stitched immediately. Antiseptics, hemp seed oil, hemp leaf oil, and cannabidiol oil were then administered to the rats for 10 days to provide antisepsis. The rats were sacrificed 24 hours after the last administration, and testicular tissues were removed. Testicular tissues were used for histopathological examination and biochemical analysis, while epididymal tissue was used for sperm analysis. According to the results, the MDA level in the antiseptic-administered group was higher than in the other experimental groups (p<0.05). Levels of SOD, CAT activities, and GSH content were found to be lower in the antiseptic group than in the hemp seed oil, hemp leaf oil, and cannabidiol oil groups (p<0.05). In testicular histology, the SEED group had the highest Johnsen score, and the antiseptic group had the lowest score (p<0.05). While JAK, P-JAK2, STAT3, PSTAT3, and NF-κB were generally higher in the antiseptic group compared to the other groups, they were lower in the SEED group. Additionally, sperm total motility rate and epididymal sperm density were highest in the SEED group (p<0.05). As a result, it was determined that cannabidiol seed oil had a good effect on testicular histology and sperm quality in male rats during the wound healing process.
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Authors and Affiliations

G.D. Akarsu
1 2
E. Erbaş
3
S.A. Akarsu
4

  1. Division of Molecular Medicine, Laboratory for Advanced Genomics, Institut Ruđer Bošković, Zagreb, Croatia
  2. Department of Pharmacy Services, Vocational School of Health Servıces, Yozgat Bozok Unıversıty, Atatürk Road 7.Km, Bilal Şahin West Campus 66100, Yozgat, Turkey
  3. Department of Histology and Embryology, Faculty of Veterinary Medicine, Ataturk University, Yakutiye 25030, Erzurum, Turkey
  4. Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Yakutiye 25030, Ataturk University, Erzurum, Turkey

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