The aim of this work was to investigate the influence of distilled water flowrate in two different porousmembrane modules on the size of generated nitrogen nanobubbles. Modules had different diameter and number of membrane tubes inside the module. As bubbles are cut off from the membrane surface by a shear stress induced by the liquid flow, the change in the linear liquid velocity should result in a change of the generated bubble diameter. For both modules, higher flowrate of liquid induced generation of smaller bubbles, which was consistent with our expectations. This effect can help us in generation of bubbles of desired size.
In this paper, three methods of sterilisation are compared to determine their usability in nanobubble dispersion sterilisation: filtration, thermal sterilisation and sonication (in two systems: using a sonotrode and sonication bath). Nanobubble dispersions are most commonly generated in non-sterile systems which precludes them from use in most biological research. As a result of this study, filtration was chosen as the best method for nanobubble sterilisation.