Trinexapac-ethyl is one of the newest growth regulators used in agriculture and horticulture. As a most growth retardants it acts by inhibiting gibberellin (GA) biosynthesis. A field study was conducted to determine the effects of trinexapac-ethyl on growth of winter wheat. Trinexapac ethyl was used alone (75 g a.i./ha and 125 g a.i./ha) and in the mixture with chlorocholine chloride (50 g a.i./ha + 675 g a.i./ha) at the 2nd node stage. Trinexapac-ethyl and its mixture with CCC activity was weather dependent. Their influence on the crop was strictly related to the temperature and rainfall during an individual year of trials. Plant growth regulators much more influenced winter wheat plants in abundant rainfall and higher temperature conditions. Lodging was not observed during the experiment.