@ARTICLE{Azeem_Hajra_Molecular_2024,
 author={Azeem, Hajra and Ali, Amjad and Perveen, Rashida and ud din Umar, Ummad and Zakria, Muhammad and Ölmez, Fatih and Azeem, Muhammad Usman},
 volume={vol. 64},
 number={No 2},
 journal={Journal of Plant Protection Research},
 pages={139-148},
 howpublished={online},
 year={2024},
 publisher={Committee of Plant Protection PAS},
 publisher={Institute of Plant Protection – National Research Institute},
 abstract={The prevalence of cotton leaf curl disease (CLCuD) has significantly hampered chili (Capsicum spp.) production, presenting a formidable challenge in Pakistan. During a chili field survey in 2018, distinct symptoms, including stunted growth, yellowing, and severe leaf curling, were observed on several plants. Subsequently, a comprehensive sampling effort was undertaken, collecting a total of 39 symptomatic samples from diverse locations across Multan, Punjab. The DNA extraction from these samples was conducted at the plant virology laboratory at Bahauddin Zakariya University, Multan, marking a crucial step in the investigation of this debilitating disease and its impact on chili production in the region. Molecular analysis with PCR using Av/Ac Core, Beta 01/02, and CLCuMuBF11/R33 primers confirmed begomovirus infection in chili plants. Positive amplification demonstrated a 71.79% infection rate, with 579 bp, 1.4 kb, and 481 bp amplicons for Av/Ac Core, Beta 01/02, and CLCuMuBF11/R33, respectively. Sequencing identified cotton leaf curl Multan beta-satellite (MT668934) infecting the chili plant. Effectively managing these begomoviruses is crucial to curbing their multiplication and protecting vital crops like chili. Addressing the distributions of beta-satellites in agricultural fields, particularly chili, is imperative to prevent further viral spread.},
 type={Article},
 title={Molecular characterization of cotton leaf curl Multan beta-satellite occurance on chili plants in Multan, Pakistan},
 URL={http://czasopisma.pan.pl/Content/131450/PDF-MASTER/OA_06_JPPR_64_2_1756_Azeem.pdf},
 doi={10.24425/jppr.2024.150248},
 keywords={begomovirus, cotton leaf curl virus, Geminiviridae, phylogenetic analysis, stunted growth},
}