@ARTICLE{Yu_Z.R._TaqMan-based_2024,
 author={Yu, Z.R. and Shao, Y. and Chen, Z. and Zhang, Y. and Cheng, F.Y. and Liu, H. and Wang, Z.Y. and Tu, J. and Song, X.J. and Qi, K.Z.},
 volume={vol. 27},
 number={No 4},
 pages={641–644},
 journal={Polish Journal of Veterinary Sciences},
 howpublished={online},
 year={2024},
 publisher={Polish Academy of Sciences Committee of Veterinary Sciences},
 publisher={University of Warmia and Mazury in Olsztyn},
 abstract={The aim of this study was to develop a rapid, sensitive and highly specific TaqMan quantita­tive real-time polymerase chain reaction PCR (qPCR) assay for porcine circovirus-like virus (PCLV). The primers and probe were designed based on the conserved regions of the PCLV ORF4 gene. The assay has a good detection performance (y = -3.3257x + 1.482, R2 = 0.9905), with a limit of detection of 10 copies, which was 100 times more sensitive than conventional PCR (cPCR). No cross-reactivity was observed with other common viruses. The intra- and inter-assay coefficients of variation were less than 1.25%. 36 fecal samples were analyzed using this method, detecting a positivity rate of 8.33% (3/36) that was higher than the cPCR detected. In summary, the established assay for PCLV detection has high specificity, sensitivity, and reproducibility and can be used as a tool for clinical diagnosis and epidemiological investigation.},
 title={TaqMan-based quantitative real-time polymerase chain reaction assay to detect porcine circovirus-like virus},
 type={Article},
 URL={http://czasopisma.pan.pl/Content/133660/PDF-MASTER/16%20_%20Yu%20_%20Short.pdf},
 doi={10.24425/pjvs.2024.152954},
 keywords={porcine circovirus-like virus, detection, ORF4 gene, TaqMan quantitative real-time PCR},
}