@ARTICLE{Cajza_Mieczysław_Improved_2003,
 author={Cajza, Mieczysław and Folkman, Wojciech},
 volume={vol. 43},
 number={No 4},
 journal={Journal of Plant Protection Research},
 pages={375-380},
 howpublished={online},
 year={2003},
 publisher={Committee of Plant Protection PAS},
 publisher={Institute of Plant Protection – National Research Institute},
 abstract={In performed experiments, insoluble polyvinylpolypyrrolidone, PVPP as an additive to the extraction buffer was used for isolation of total nucleic acids from hop plants and grapevine in order to obtain templates useful for detection ofHLVd and HSVd by means ofRT-PCR. Addition of2% of PVPP to the original GTC buffer (Chomczynski and Sacchi, 1987) appeared to be the most favorable. Due to PVPP addition, the protocol of extraction of nucleic acids was simplified by shortening of isolation time and reduction of expenses. However, application of the simplified method for obtaining of templates that guaranteed full repeatability of test results was limited to the spring and early summer season.},
 type={Article},
 title={Improved method of isolation of total nucleic acids from hop plants and grapevine before the RT-PCR by addition of polyvinylpolypyrrolidone},
 URL={http://czasopisma.pan.pl/Content/130978/PDF-MASTER/15_PLANT_43_4_2003_Cajza_Improved.pdf},
 keywords={PVP, PVPP, polyvinylpolypyrrolidone, RT-PCR, reverse transcription-polymerase chain reaction, HLVd, HSVd},
}