TY - JOUR N2 - Maize dwarf mosaic virus (MDMV) is a serious and widespread virus pathogen of maize plants. This +ssRNA virus belongs to the Potyvirus genus in the Potyviridae family. Together with sugarcane mosaic virus (SCMV) it causes one of the most important viral diseases on maize crops in the world – maize dwarf mosaic. Both viruses are transmitted in the same non-persistent manner by several aphid species. They induce similar symptoms of leaf mosaic or mottling, stunting and a reduction in plant weight and grain yield. Available MDMV diagnostics include primarily commercialized enzyme-linked immunosorbent assays (ELISA) and reverse transcription-polymerase chain reactions (RT-PCR). Here, laborsaving reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay was optimized for identification of genetically different MDMV isolates. For this purpose, primer sets, MDMVF3/MDMVB3 and MDMVFIP/MDMVBIP amplifying fragments of coat protein coding sequence of MDMV, were used. The specificity of the reaction was verified using three MDMV (-P1, -Sp, -PV0802-DSMZ) and three SCMV (-P1, -PV0368- -DSMZ, -PV1207-DSMZ) isolates. Obtained products were visualised by DNA staining, electrophoretic separation as well as by real-time monitoring of the reaction. The sensitivity of RT-LAMP and conventional RT-PCR reactions was comparable. Both methods could detect virus as low as 550 fg · μl–1 of total RNA. This technique has application value for screening MDMV by phytosanitary services. L1 - http://czasopisma.pan.pl/Content/124366/PDF-MASTER/RC_02_JPPR_62_3_1418_Trzmiel.pdf L2 - http://czasopisma.pan.pl/Content/124366 PY - 2022 IS - No 3 EP - 306 DO - 10.24425/jppr.2022.142138 KW - MDMV KW - SCMV KW - identification KW - maize KW - RT-LAMP technique A1 - Trzmiel, Katarzyna A1 - Hasiów-Jaroszewska, Beata PB - Committee of Plant Protection PAS PB - Institute of Plant Protection – National Research Institute VL - vol. 62 DA - 2022.09.19 T1 - Development of reverse transcription-loop-mediated isothermal amplification assay for the detection of genetically different isolates of maize dwarf mosaic virus SP - 302 UR - http://czasopisma.pan.pl/dlibra/publication/edition/124366 T2 - Journal of Plant Protection Research ER -