Investigation of Salmonella spp. and Escherichia coli in the Snake-eyed lizard ( Ophisops elegans) (Sauria, Lacertidae) in the Çankırı Province of Turkey

Journal title

Polish Journal of Veterinary Sciences




vol. 26


No 1


Tarhane, S. : Veterinary Department, Eldivan Vocational School of Health Services, Çankırı Karatekin University, 18100, Çankırı, Turkey ; Bozkurt, E. : Veterinary Department, Eldivan Vocational School of Health Services, Çankırı Karatekin University, 18100, Çankırı, Turkey ; Büyük, F. : Department of Microbiology, Faculty of Veterinary Medicine, Kafkas University, 36100, Kars, Turkey



bacterial infection ; PCR ; reptilia ; Turkey

Divisions of PAS

Nauki Biologiczne i Rolnicze




Polish Academy of Sciences Committee of Veterinary Sciences ; University of Warmia and Mazury in Olsztyn



Allen T, Murray KA, Zambrana-Torrelio C, Morse SS, Rondinini C, Di Marco M, Breit N, Olival KJ, Daszak P (2017) Global hotspots and correlates of emerging zoonotic diseases. Nat Commun 8: 1124.
Antaki-Zukoski EM, Li X, Pesavento PA, Nguyen THB, Hoar BR, Atwill ER (2018) Comparative pathogenicity of wildlife and bovine Escherichia coli O157:H7 strains in experimentally inoculated neonatal Jersey calves. Vet Sci 5: 88.
Babacan O (2023) Investigating the presence and antibiotic susceptibilities of Escherichia coli O157 and Listeria monocytogenes in ruminant feces and feed in Balıkesir province. Ankara Univ Vet Fak Derg, 70: 1-30.
Bakaloudis DE, Iezekiel S, Vlachos CG, Bontzorlos VA, Papakosta M, Birrer S (2012) Assessing bias in diet methods for the Long-legged Buzzard Buteo rufinus. J Arid Environ 77: 59-65.
Bautista-Trujillo GU, Gutiérrez-Miceli FA, Mandujano-Garcia L, Oliva-Llaven MA, Ibarra-Martinez C, Mendoza-Nazar P, Ruiz-Sesma B, Tejeda-Cruz C, Pérez-Vázquez LC, Pérez-Batrez JE, Vidal JE, Gutiérrez-Jiménez J (2020) Captive Green iguana carries diarrheagenic Escherichia coli pathotypes. Front Vet Sci 7: 99.
Bender JB, Shulman SA (2004) Reports of zoonotic disease outbreaks associated with animal exhibits and availability of recommendations for preventing zoonotic disease transmission from animals to people in such settings. J Am Vet Med Assoc 224: 1105-1109.
Chambers DL, Hulse AC (2006) Salmonella serovars in the herpetofauna of Indiana County, Pennsylvania. Appl Environ Microbiol 72: 3771-3773.
Dec M, Stepien-Pysniak D, Szczepaniak K, Turchi B, Urban-Chmiel R (2022) Virulence profiles and antibiotic susceptibility of Escherichia coli strains from pet reptiles. Pathogens 11: 127.
Dróżdż M, Małaszczuk M, Paluch E, Pawlak A (2021) Zoonotic potential and prevalence of Salmonella serovars isolated from pets. Infect Ecol Epidemiol 11: 1975530.
Ebani VV (2017) Domestic reptiles as source of zoonotic bacteria: A mini review. Asian Pac J Trop Med 10: 723-728.
Eekhout X (2010) Sampling amphibians and reptiles. In: Eymann J, Degreef J, Hauser C, Monje JC, Samyn Y, Vandenspiegel D (eds) Manual on field recording techniques and protocols for all taxa biodiversity inventories. Belgium: Belgian National Focal Point to The Global Taxonomy Initiative, 8, pp 530-557.
Engdaw TA, Temesgen W (2016) O157:H7 serotype of Escherichia coli as an important emerging zoonosis. Int J Microbiol Res 7: 9-17.
Gambi L, Rossini R, Menandro ML, Franzo G, Valentini F, Tosi G, D’Incau M, Fiorentini L (2022) Virulence factors and antimicrobial resistance profile of Escherichia coli isolated from laying hens in Italy. Animals (Basel) 12: 1812.
Geue L, Löschner U (2002) Salmonella enterica in reptiles of German and Austrian origin. Vet Microbiol 84: 79-91.
Goldwater PN, Bettelheim KA (2012) Treatment of enterohemorrhagic Escherichia coli (EHEC) infection and hemolytic uremic syndrome (HUS). BMC Med 10:12.
Gyles CL (2007) Shiga toxin-producing Escherichia coli: An overview. J Anim Sci 85: E45-62.
Hanson R, Kaneene JB, Padungtod P, Hirokawa K, Zeno C (2003) Prevalence of Salmonella and E. coli, and their resistance to antimicrobial agents, in farming communities in northern Thailand. Southeast Asian J Trop Med Public Health 33: 120-126.
Hawwas HA, Aboueisha AM, Fadel HM, El-Mahallawy HS (2022) Salmonella serovars in sheep and goats and their probable zoonotic potential to humans in Suez Canal Area, Egypt. Acta Vet Scand 64: 17.
İnci A, Doğanay M, Özdarendeli A, Düzlü Ö, Yildirim A (2018) Overview of zoonotic diseases in Turkey: The one health concept and future threats. Turkiye Parazitol Derg 42: 39-80.
Kar J, Barman TR, Sen A, Nath SK (2017) Isolation and identification of Escherichia coli and Salmonella sp. from apparently healthy turkey. Int J Adv Res Biol Sci 4: 72-78.
Lupolova N, Dallman TJ, Holden NJ, Gally DL (2017) Patchy promiscuity: Machine learning applied to predict the host specificity of Salmo-nella enterica and Escherichia coli. Microb Genom 3: e000135.
Mermin J, Hutwagner L, Vugia D, Shallow S, Daily P, Bender J Koehler J, Marcus R, Angulo FJ, Emerging Infections Program FoodNet Working Group (2004) Reptiles, amphibians, and human Salmonella infection: A population‐based, case‐control study. Clin Infect Dis 38: S253-S261.
Middleton DM, Minot EO, Gartrell BD (2010) Salmonella enterica serovars in lizards of New Zealand’s offshore islands. N Z J Ecol 34: 247-252.
Moxley RA (2004) Escherichia coli 0157:H7: An update on intestinal colonization and virulence mechanisms. Anim Health Res Rev 5: 15-33.
Nielsen TP, Bull CM (2016) Impact of foxes digging for the pygmy bluetongue lizard (Tiliqua adelaidensis). Trans R Soc S Aust 140: 228-233.
Ogunleye AO, Ajuwape AT, Alaka OO, Adetosoye AI (2013) Characterization of a Salmonella enterica serotype pullorum isolated from a lizard co-habitating with poultry. Afr J Microbiol Res 7: 1215-1221.
Oraie H, Rahimian H, Rastegar-Pouyani N, Rastegar-Pouyani E, Ficetola GF, Yousefkhani SS, Khosravani A (2014) Distribution pattern of the Snake-eyed Lizard, Ophisops elegans Ménétriés, 1832 (Squamata: Lacertidae), in Iran. Zool Middle East 60: 125-132.
Poulin B, Lefebvre G, Ibanez R, Jaramillo C, Hernendez C, Rand AS (2001) Avian predation upon lizards and frogs in a neotropical forest understorey. J Trop Ecol 17: 21-40.
Pui CF, Wong WC, Chai LC, Tunung R, Jeyaletchumi P, Noor Hidayah MS, Ubong A, Farinazleen MG, Cheah YK, Son R (2011) Salmonella: A foodborne pathogen. Int Food Res J 18: 465-473.
Rahn K, De Grandis SA, Clarke RC, McEwen SA, Galán JE, Ginocchio C, Curtiss R 3rd, Gyles CL (1992) Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella. Mol Cell Probes 6: 271-279.
Ramos CP, Santana JA, Morcatti Coura F, Xavier RG, Leal CA, Oliveira CA Jr, Heinemann MB, Lage AP, Lobato FC, Silva RO (2019) Identification and characte-rization of Escherichia coli, Salmonella spp., Clostridium perfringens, and C. difficile isolates from reptiles in Bra-zil. Biomed Res Int 2019:9530732.
Spickler AR. (2013) Reptile-Associated Salmonellosis. Retrieved from /factsheets.php
Şahan Ö, Aral EM, Aden MM, Aksoy A, Yılmaz Ö, Jahed R, Akan M (2016) Distribution and antibiotic resistance of Salmonella isolates from broiler enterprices in Turkey. Ankara Univ Vet Fak Derg 63: 1-6.
Thaller MC, Migliore L, Marquez C, Tapia W, Cedeño V, Rossolini GM, Gentile G (2010) Tracking acquired antibiotic resistance in commensal bacteria of Galápagos land iguanas: No man, no resistance. PLoS One 5: e8989.
Trochimchuk T, Fotheringham J, Topp E, Schraft H, Leung KT (2003) A comparison of DNA extraction and purification methods to detect Escherichia coli O157:H7 in cattle manure. J Microbiol Methods 54: 165-175.
Valdez JW (2021) Using google trends to determine current, past, and future trends in the reptile pet trade. Animals (Basel) 11: 676.
Walters SP, Gannon VPJI, Field KG (2007) Detection of Bacteroidales fecal indicators and the zoonotic pathogens E. coli O157:H7, Salmo-nella, and Campylobacter in river water. Environ Sci Technol 41: 1856-1862.
Wang RF, Cao WW, Cerniglia CE (1997) A universal protocol for PCR detection of 13 species of foodborne pathogens in foods. J Appl Microbiol 83: 727-736.
Wheeler E, Hong PY, Bedon LC, Mackie RI (2012) Carriage of antibiotic-resistant enteric bacteria varies among sites in Galapagos reptiles. J Wildl Dis 48: 56-67.
Wooley RE, Ritchie BW, Currin MF, Chitwood SW, Sanchez S, Crane MM, Lamberski N (2001) In vitro inhibition of Salmonella organisms isolated from reptiles by an inactivated culture of microcin-producing Escherichia coli. Am J Vet Res 62: 1399-1401.
Zając M, Wasyl D, Różycki M, Bilska-Zając E, Fafiński Z, Iwaniak W, Krajewska M, Hoszowski A, Konieczna O, Fafinska P, Szulowski K (2016) Free-living snakes as a source and possible vector of Salmonella spp. and parasites. Eur J Wildl Res 62: 161-166.






DOI: 10.24425/pjvs.2023.145009