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Title

Virological investigation on the presence of CPV-2 in the Middle Black Sea Region of Turkey

Journal title

Polish Journal of Veterinary Sciences

Yearbook

2026

Volume

Vol. 29

Issue

No. 1

Authors

Kurucay, H.N. ; Gumusova, S.

Affiliation

Kurucay, H.N. : Ondokuz Mayıs University, Kurupelit Campus Faculty of Veterinary Medicine, Department of Virology, 55200 Atakum, Samsun, Turkiye ; Gumusova, S. : Ondokuz Mayıs University, Kurupelit Campus Faculty of Veterinary Medicine, Department of Virology, 55200 Atakum, Samsun, Turkiye

Keywords

CPV-2 ; dog ; isolation ; Next-Generation Sequencing ; Real-Time PCR

Divisions of PAS

Nauki Biologiczne i Rolnicze

Coverage

5–15

Publisher

Polish Academy of Sciences Committee of Veterinary Sciences ; University of Warmia and Mazury in Olsztyn

Bibliography

  • Abayli H, Aslan O, Tumer KC, Can-Sahna K, Tonbak S (2022) Predominance and first complete genomic characterization of canine parvovirus 2b in Turkey. Arch Virol 167: 1831-1840.
  • Adeyemo AA, Aiki-Raji CO, Akinniyi OO, Fagbohun OA (2024) Molecular Epidemiology of Canine Parvovirus in Nigeria. Afr J Biomed Res 27: 217-224.
  • Akkutay-Yoldar A, Koc B (2020) Phylogenetic analysis of canine parvoviruses from Turkey. Med Weter 76: 17-23.
  • Alexis VA, V Sonia, Daniela S, Miguel G, Timothy H, Valentina F, Lisette L, Leonardo S (2021) Molecular analysis of full-length VP2 of canine parvovirus reveals antigenic drift in CPV-2b and CPV-2c variants in Central Chile. Animals 11: 2387.
  • Ammar EF, Hegazy YM, Al-Gaabary M, Mosad SM, Salem M, Marzok M, Housawi F, Al-Ali M, Alhaider A, Tahoun A (2024) Epidemiological, and molecular investigation of Canine parvovirus-2 infection in Egypt. J Vet Sci 25: e56.
  • Battilani M, Modugno F, Mira F, Purpari G, Di Bella S, Guercio A, Balboni A (2019) Molecular epidemiology of canine parvovirus type 2 in Italy from 1994 to 2017: recurrence of the CPV-2b variant. BMC Vet Res 15: 393.
  • Buonavoglia C, Martella V, Pratelli A, Tempesta M, Cavalli A, Buonavoglia D, Bozzo G, Elia G, Decaro N, Carmichael L (2001) Evidence for evolution of canine parvovirus type 2 in Italy. J Gen Virol 82: 3021-3025.
  • Charoenwongpaiboon T, Sommanat N, Wangpaiboon K, Puangpathanachai M, Pongsawasdi P, Pichyangkura R (2024) Improving the thermostability and modulating the inulin profile of inulosucrase through rational glycine-toproline substitution. RSC Advances 14: 2346-2353.
  • Clark NJ, Seddon JM, Kyaw-Tanner M, Al-Alawneh J, Harper G, McDonagh P, Meers J (2018) Emergence of canine parvovirus subtype 2b (CPV-2b) infections in Australian dogs. Infect Genet Evol 58: 50-55.
  • Decaro N, Buonavoglia C (2012) Canine parvovirus – a review of epidemiological and diagnostic aspects, with emphasis on type 2c. Vet Microbiol 155: 1-12.
  • Decaro N, Buonavoglia CB, Barrs VR (2020) Canine parvovirus vaccination and immunisation failures: Are we far from disease eradication?. Vet Microbiol 247: 108760.
  • Decaro N, Elia G, Martella V, Desario C, Campolo M, Di Trani L, Tarsitano E. Tempesta M, Buonavoglia C (2005) A real-time PCR assay for rapid detection and quantitation of canine parvovirus type 2 in the feces of dogs. Vet Microbiol 105: 19-28.
  • Dema A, Ganji VK, Yella NR, Putty K (2021) A novel one-step amplification refractory mutation system PCR (ARMSPCR) for differentiation of canine parvovirus-2 variants. Virus Genes 57: 426-433. 
  • Diakoudi G, Lanave G, Berjaoui S, Desario C, Di Teodoro G, Vasinioti VI, Pellegrini F, Defourny SV, Salucci S, Cocco A, Lorusso A, Martella V, Decaro N (2024) Feline Panleukopenia Virus in a Marsican Brown Bear and Crested Porcupine, Italy, 2022–2023. Emerg Infect Dis 30: 2655-2659.
  • Dincer E (2017) Molecular characterization and phylogenetic analysis of canine parvovirus 2 in dogs, Mersin Province, Turkey. Etlik Vet Mikrobiyol Derg 28: 96-100.
  • Doan HT, Le XT, Do RT, Nguyen KT, Le TH (2021) Canine parvovirus type 2c in Vietnam continues to produce distinct descendants with new mutations restricted to Vietnamese variants. Arch Virol 166: 1741-1749.
  • Dunowska M, Bain H, Bond S (2025) Molecular survey of canine parvovirus type 2: the emergence of subtype 2c in New Zealand. N Z Vet J 73: 178-186.
  • Elbaz E, El-Tholoth M, Elfadl EA, Mosad SM (2021) Molecular investigation on the presence of canine parvovirus in Egypt. Comp Immunol Microbiol Infect Dis 74: 101576.
  • Ford J, McEndaffer L, Renshaw R, Molesan A, Kelly K (2017) Parvovirus Infection Is Associated with Myocarditis and Myocardial Fibrosis in Young Dogs. Vet Pathol 54: 964-971. 
  • Fu P, He D, Cheng X, Niu X, Wang C, Fu Y, Li K, Zhu H, Lu W, Wang J, Chu, B (2022) Prevalence and characteristics of canine parvovirus type 2 in Henan Province, China, Microbiol Spectr 10: e0185622.
  • Galvis CC, Jimenez-Villegas T, Romero DP, Velandia A, Taniwaki S, de Souza Silva SO, Brandão P, Santana-Clavijo NF (2021) Molecular diversity of the VP2 of Carnivore protoparvovirus 1 (CPV-2) of fecal samples from Bogotá. J Vet Sci 23: e14.
  • Gerlach M, Proksch AL, Dörfelt R, Unterer S, Hartmann K (2020) Therapy of canine parvovirus infection – review and current insights. Tierarztl Prax Ausg K Kleintiere Heimtiere 48: 26-37
  • Grecco S, Condon E, Bucafusco D, Bratanich AC, Panzera Y, Pérez R (2024) Comparative genomics of canine parvovirus in South America: Diversification patterns in local populations. Infect Genet Evol 123: 105633.
  • Hao X, Li Y, Xiao X, Chen B, Zhou P, Li S (2022) The changes in canine parvovirus variants over the years. Int J Mol Sci 23: 11540
  • Hasircioglu S, Aslim HP (2022) Comparative Detection of Canine Parvovirus by Differential PCR, Conventional PCR and Nested PCR tests: Detecting Antigenic Variants by Differential PCR. TURJAF 10: 254-259.
  • Hartmann SR, Charnesky AJ, Früh SP, López-Astacio RA, Weichert WS, DiNunno N, Cho SH, Bator CM, Parrish CR, Hafenstein SL (2023) Cryo EM structures map a post vaccination polyclonal antibody response to canine parvovirus. Commun Biol 6: 955.
  • Jantafong T, Ruenphet S, Garner HR, Ritthipichai K (2022) Tracing the genetic evolution of canine parvovirus type 2 (CPV-2) in Thailand. Pathogens 11: 1460.
  • Jiang H, Yu Y, Yang R, Zhang S, Wang D, Jiang Y, Yang W, Huang H, Shi C, Ye L, Yang G, Wang J, Wang C (2021) Detection and Molecular Epidemiology of Canine Parvovirus Type 2 (CPV-2) Circulating in Jilin Province, Northeast China. Comp Immunol Microbiol Infect Dis 74: 101602.
  • Karapinar Z, Dincer E, Ozkan C (2018) The investigation and phylogenetic analysis of canine parvovirus 2 infection from blood and rectal swab samples from dogs in Van Province, Turkey. Van Vet J 29: 83-86.
  • Kurucay HN, Tamer C, Muftuoglu B, Elhag AE, Gozel S, Cicek-Yildiz Y, Demirtas S, Ozan E, Albayrak H, Gumusova S, Yazici Z (2023) First isolation and molecular characterization of canine parvovirus-type 2b (CPV-2b) from red foxes (Vulpes vulpes) living in the wild habitat of Turkey. Virol J 20: 27.
  • Kwan E, Carrai M, Lanave G, Hill J, Parry K, Kelman M, Meers J, Decaro N, Beatty JA, Martella V, Barrs VR (2021) Analysis of Canine Parvoviruses Circulating in Australia Reveals Predominance of Variant 2b and Identifies Feline Parvovirus-like Mutations in the Capsid Proteins. Transbound Emerg Dis 68: 656–666.
  • Li G, Ji S, Zhai X, Zhang Y, Liu J, Zhu M, Zhou J, Su S (2017) Evolutionary and genetic analysis of the VP2 gene of canine parvovirus. BMC Genomics 18: 534.
  • Li H, Zhou H, Li L, Han X, Shang Q, Zhang C, Zhou M, Fu ZF, Zhao J, Zhao L (2025) Epidemiological status and genome characteristics of canine parvovirus in China from 2022 to 2024. Arch Virol 170: 124
  • Li S, Chen X, Hao Y, Zhang G, Lyu Y, Wang J, Liu W, Qin T (2022) Characterization of the VP2 and NS1 Genes from Canine Parvovirus Type 2 (CPV-2) and Feline Panleukopenia Virus (FPV) in Northern China. Front Vet Sci 9: 934849.
  • Liu C, Gao J, Li H, Sun F, Liang H, Liu H, Yi J (2021) Phylogenetic characteristics of canine parvovirus type 2c variant endemic in Shanghai, China. Viruses 13: 2257.
  • Maganga GD, Labouba I, Milendz Ikapi SZ, Nkili-Meyong AA, Ngonga Dikongo AM, Boundenga L, Ngoubangoye B, Memvie C, Kumulungui BS (2023) Molecular characterization of canine parvovirus variants CPV-2a and CPV-2c, associated with vaccinated dogs at Libreville, Gabon. Viruses 15: 1169.
  • Mazzaferro EM (2020) Update on Canine Parvoviral Enteritis. Vet Clin North Am Small Anim Pract 50: 1307-1325.
  • Mia M, Hasan M (2021) Update on canine parvovirus infection: a review from the literature. Vet Scie Res Rev 7: 92-100.
  • Mech LD, Goyal SM, Paul WJ, Newton WE (2008) Demographic effects of canine parvovirus on a free-ranging wolf population over 30 years. J Wildl Dis 44: 824-836.
  • Mekky RH, Shoulah S, Moustafa A, Selim A, Marawan MA (2024) Prevalence and molecular characterization of canine parvovirus-2 in dogs in Giza Governorate, Egypt. J Adv Vet 14: 253-256.
  • Mira F, Canuti M, Purpari G, Cannella V, Di Bella S, Occhiogrosso L, Schiro G, Chiaramonte G, Barreca S, Pisano P, Lastra A, Decaro N, Guercio A (2019) Molecular characterization and evolutionary analyses of carnivore protoparvovirus 1 NS1 gene. Viruses 11: 308.
  • Mira F, Schirò G, Franzo G, Canuti M, Purpari G, Giudice E Decaro N, Vicari D, Antoci F, Castronovo C, Guercio A (2024) Molecular epidemiology of canine parvovirus type 2 in Sicily, southern Italy: a geographical island, an epidemiological continuum. Heliyon 10: e26561
  • Mittal M, Chakravarti S, Mohapatra JK, Chug PK, Dubey R, Upmanuyu V, Narwal PS, Kumar A Churamani CP, Kanwar NS (2014) Molecular typing of canine parvovirus strains circulating from 2008 to 2012 in an organized kennel in India reveals the possibility of vaccination failure. Infect Genet Evol 23: 1-6.
  • Pénzes JJ, SöderlundVenermo M, Canuti M, EisHübinger AM, Hughes J, Cotmore SF, Harrach B (2020) Reorganizing the family Parvoviridae: a revised taxonomy independent of the canonical approach based on host association. Arch Virol 165: 2133-2146
  • Pereira CA, Monezi TA, Mehnert DU, D’Angelo M, Durigon EL (2000) Molecular characterization of canine parvovirus in Brazil by polymerase chain reaction assay. Vet Microbiol 75: 127-133.
  • Polat PF, Sahan A, Aksoy G, Timurkan MO, Dincer E (2019) Molecular and restriction fragment length polymorphism analysis of canine parvovirus 2 (CPV-2) in dogs in southeast Anatolia, Turkey. Onderstepoort J Vet Res 86: 1-8
  • Reddy H, Srinivas MV, Vasu J, Prabavathy A, Dhodapkar R, Mukhopadhyay H K (2024) Whole-genome sequence analysis of canine parvovirus reveals replacement with a novel CPV-2c strain throughout India. Arch Virol 169: 189.
  • Zhu Q, Yuan P, Li ZJ, Diao FF, Kang S, Li LJ, Shijin J, Xie ZJ (2021) Nucleotide sequences of the infectious DNA clones of two mink enteritis virus isolates exhibit the diversity of the terminal palindromic sequences and predicted secondary structures. Virus Res 305: 198574
  • Saltik HS, Koc BT (2024) First identification of canine parvovirus‐2a/2b variant in unvaccinated domestic dogs with gastrointestinal signs in Türkiye. Vet Med Sci 10: e1523.
  • Silva LM, Santos MR, Carvalho JA, Carvalho OV, Favarato ES, Fietto JL, Bressan GC, Silva-Júnior A (2022) Molecular analysis of the full-length VP2 gene of Brazilian strains of canine parvovirus 2 shows genetic and structural variability between wild and vaccine strains. Virus Res 313: 198746.
  • Temeeyasen G, Sharafeldin TA, Lin CM, Hause BM (2022) Spillover of Canine Parvovirus Type 2 to Pigs, South Dakota, USA, 2020. Emerg Infect Dis 28: 471-473.
  • Takano T, Hamaguchi S, Hasegawa N, Doki T, Soma T (2021) Predominance of canine parvovirus 2b in Japan: an epidemiological study during 2014-2019. Arch Virol 166: 3151-3156
  • Temizkan MC, Temizkan SS (2023) Canine Parvovirus in Turkey: First Whole-Genome Sequences, Strain Distribution, and Prevalence. Viruses 15: 957.
  • Timurkan M, Oguzoglu T (2015) Molecular characterization of canine parvovirus (CPV) infection in dogs in Turkey. Vet Ital 51: 39-44.
  • Tuteja D, Banu K, Mondal B (2022) Canine parvovirology – a brief updated review on structural biology, occurrence, pathogenesis, clinical diagnosis, treatment and prevention. Comp Immunol Microbiol Infect Dis 82: 101765.
  • Voorhees IE, Lee H, Allison AB, Lopez-Astacio R, Goodman LB, Oyesola OO, Omobowale O, Fagbohun O, Dubovi EJ, Hafenstein SL, Holmes EC, Parrish CR (2019) Limited intrahost diversity and background evolution accompany 40 years of canine parvovirus host adaptation and spread. J Virol 94: e01162-19.
  • Wiedemann C, Kumar A, Lang A, Ohlenschläger O (2020) Cysteines and disulfide bonds as structure-forming units: insights from different domains of life and the potential for characterization by NMR. Front Chem 8: 280.
  • Wong JW, Ho SY, Hogg PJ (2011) Disulfide bond acquisition through eukaryotic protein evolution. Mol Biol Evol 28: 327-334.
  • Yip HY, Peaston A, Woolford L, Khuu SJ, Wallace G, Kumar RS, Patel K, Azari AA, Akbarzadeh M, Sharifian M, Amanollahi R, Jozani RJ, Khabiri A, Hemmatzadeh F (2020) Diagnostic challenges in canine parvovirus 2c in vaccine failure cases. Viruses 12: 980.
  • Zhou P, Zeng W, Zhang X, Li S (2017) The genetic evolution of canine parvovirus – a new perspective. PloS One 12: e0175035.
  • Zhou H, Cui K, Su X, Zhang H, Xiao B, Li S, Yang B (2024) Overview of recent advances in canine parvovirus research: current status and future perspectives. Microorganisms 13: 47.

Date

17.03.2026

Type

Article

Identifier

DOI: 10.24425/pjvs.2026.158496
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