Details

PDF BIBTEX RIS

Title

Advances in the application of natural bioactive compounds for the prevention and control of porcine epidemic diarrhea virus via the oxidative stress pathway

Journal title

Polish Journal of Veterinary Sciences

Yearbook

2026

Volume

Vol. 29

Issue

No. 1

Authors

Zou, H. ; An, W.T. ; Huang, S.L. ; Luo, G. ; Mu, Z.P.

Affiliation

Zou, H. : College of Animal Science and Technology, Chongqing Three Gorges Vocational College, Chongqing 404100, China ; An, W.T. : College of Animal Science and Technology, Chongqing Three Gorges Vocational College, Chongqing 404100, China ; Huang, S.L. : College of Animal Science and Technology, Chongqing Three Gorges Vocational College, Chongqing 404100, China ; Luo, G. : Wanzhou Center for Animal Husbandry Industry Development of Chongqing, Chongqing 404100, China ; Mu, Z.P. : College of Animal Science and Technology, Chongqing Three Gorges Vocational College, Chongqing 404100, China

Keywords

porcine epidemic diarrhea virus ; natural bioactive compounds ; oxidative stress

Divisions of PAS

Nauki Biologiczne i Rolnicze

Coverage

165–174

Publisher

Polish Academy of Sciences Committee of Veterinary Sciences ; University of Warmia and Mazury in Olsztyn

Bibliography

  • Akinboye AJ, Kim K, Choi S, Yang I, Lee JG (2023). Alkaloids in food: a review of toxicity, analytical methods, occurrence and risk assessments. Food Sci Biotechnol 32: 1133-1158.
  • Aviello G, Knaus UG (2017). ROS in gastrointestinal inflammation: Rescue or sabotage? Br J Pharmacol 174: 1704-1718.
  • Bao D, Yi S, Zhao L, Zhao H, Liu J, Wei Y, Hu G, Liu, X (2024). Porcine epidemic diarrhea virus infection of porcine intestinal epithelial cells causes mitochondrial DNA release and the activation of the NLRP3 inflammasome to mediate interleukin-1β secretion. Vet Sci 11: 643.
  • Bechaux J, Gatellier P, Le Page JF, Drillet Y, Sante-Lhoutellier V (2019). A comprehensive review of bioactive peptides obtained from animal byproducts and their applications. Food Funct 10: 6244-6266.
  • Cao Y, Zhang S, Huang Y, Zhang S, Wang H, Bao W (2022). The aqueous leaf extract of M. oleifera inhibits PEDV replication through suppressing oxidative stress-mediated apoptosis. Animals 12: 458.
  • Chen L, Sun L, Liu Z, Wang H, Xu C (2016). Protection afforded by quercetin against H2O2-induced apoptosis on PC12 cells via activating PI3K/akt signal pathway. J Recept Signal Transduct Res 36: 98-102.
  • Cione E, La Torre C, Cannataro R, Caroleo MC, Plastina P, Gallelli L (2019). Quercetin, epigallocatechin gallate, curcumin, and resveratrol: From dietary sources to human MicroRNA modulation. Molecules 25: 63.
  • Da Fonseca RN, Maggioli MF, De Oliveira Hübner S, Bauermann FV (2025). Antiviral effects of flavonoids on animal viruses. Virology 610: 110596.
  • Dai J, Mumper RJ (2010). Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules 15: 7313-7352.
  • Dickson KB, Zhou J (2020). Role of reactive oxygen species and iron in host defense against infection. Front Biosci (Landmark Ed), 25: 1600-1616.
  • Duan C, Luo Y, Liang X, Wang X (2022). A review of bioactive compounds against porcine enteric coronaviruses. Viruses 14: 2217.
  • Farooq S, Ngaini Z (2025). Facile synthesis and applications of flavonoid-heterocyclic derivatives. Curr Top Med Chem 25: 47-62.
  • Forman HJ, Zhang H, Rinna A (2009). Glutathione: overview of its protective roles, measurement, and biosynthesis. Mol Aspects Med 30: 1-12.
  • Gao C, Zhang L, Ma J, Zhang H, Pang Y, Xue C, Li D, Zhao X, Gantumur MA, Hui M, Hong W, Bao Y, Dong N (2025). Exploring food-derived bioactive peptides: efficacy, mechanisms, and clinical implications in ferroptosis regulation. Trends Food Sci Tech 160: 105014.
  • Gebicka L, Krych-Madej J (2019). The role of catalases in the prevention/promotion of oxidative stress. J Inorg Biochem 197: 110699.
  • Ghasemi S, Hassanpour S, Hosseini R (2025). Protective effect of the quercetin on experimental cuprizone-induced multiple sclerosis in male C57BL/6 mice. Current Research in Physiology 8: 100146.
  • Guo J, Fang L, Ye X, Chen J, Xu S, Zhu X, Miao Y, Wang D, Xiao S (2019). Evolutionary and genotypic analyses of global porcine epidemic diarrhea virus strains. Transbound Emerg Dis 66: 111-118.
  • Hajfathalian M, Ghelichi S, García-Moreno PJ, Moltke Sørensen AD, Jacobsen C (2018). Peptides: production, bioactivity, functionality, and applications. Crit Rev Food Sci 58: 3097-3129.
  • Hall HN, Wilkinson DJ, Le Bon M (2021). Oregano essential oil improves piglet health and performance through maternal feeding and is associated with changes in the gut microbiota. Animal Microbiome 3: 2.
  • Huang C, Cui H, Li X, Askar G, Yu J, Hayat K, Zhang X, Ho CT (2025). Current progress in the ginger oleoresin: bioactivities, extraction and encapsulation technologies. Crit Rev Food Sci 66: 1-20.
  • Jia J, Liu Q, Liu H, Yang C, Zhao Q, Xu Y, Wu W (2024). Structure characterization and antioxidant activity of abalone visceral peptides-selenium in vitro. Food Chem 433: 137398.
  • Koonpaew S, Teeravechyan S, Frantz PN, Chailangkarn T, Jongkaewwattana A (2019). PEDV and PDCoV pathogenesis: the interplay between host innate immune responses and porcine enteric coronaviruses. Front Vet Sci 6: 34.
  • Kwaśny D, Borczak B, Zagrodzki P, Kapusta-Duch J, Prochownik E, Doskočil I (2025). Antioxidant activity, total polyphenol content, and cytotoxicity of various types of starch with the addition of different polyphenols. Molecules 30: 2458.
  • Li D, Jia Q, Zhao Q, Sun J, Yu J, Chen H, Sui L, Leng A, Guo P, Wang C (2025). Macrolide sesquiterpene pyridine alkaloids from the roots of tripterygium regelii and their anti-inflammatory activity. Bioorg Chem 158: 108330.
  • Li H, Wu M, Li Z, Zhang Q, Zhang X, Zhang Y, Zhao D, Wang L, Hou Y, Wu T (2024). Effect of supplementation with yeast polysaccharides on intestinal function in piglets infected with porcine epidemic diarrhea virus. Front Microbiol 15: 1378070.
  • Li L, Li H, Qiu Y, Li J, Zhou Y, Lv M, Xiang H, Bo Z, Shen H, Sun P (2024). PA-824 inhibits porcine epidemic diarrhea virus infection in vivo and in vitro by inhibiting p53 activation. J Virol 98: e0041323.
  • Li W, Hangalapura BN, van den Elzen P, van den Born E, van Kuppeveld FJ, Rottier PJ, Bosch BJ (2025). Spike gene variability in porcine epidemic diarrhea virus as a determinant for virulence. J Virol 99: e0216524.
  • Li X, Wu Y, Yan Z, Li G, Luo J, Huang S, Guo X (2024). A comprehensive view on the protein functions of porcine epidemic diarrhea virus. Genes 15: 165.
  • Li Y, Yang S, Qian J, Liu S, Li Y, Song X, Cao Q, Guo R, Zhao Y, Sun M, Hu M, Li J, Zhang X, Fan B, Li B (2025). Molecular characteristics of the immune escape of coronavirus PEDV under the pressure of vaccine immunity. J Virol 99: e0219324.
  • Liang J, Xu W, Pan X, Han S, Zhang L, Wen H, Ding M, Zhang W, Peng D (2024). Advances research in porcine enteric coronavirus therapies and antiviral drugs. Vet Quart 44: 1-49.
  • Lin CM, Saif LJ, Marthaler D, Wang Q (2016). Evolution, antigenicity and pathogenicity of global porcine epidemic diarrhea virus strains. Virus Res 226: 20-39.
  • Liu LY, Peng Q, Yang YK, Wu T, Chen LX, Lin L (2025). Seven previously undescribed steroidal alkaloids from the fruit of solanum nigrum L and their biological activities. Phytochemistry 237: 114525.
  • Liu Y (2015). Fatty acids, inflammation and intestinal health in pigs. J Anim Sci Biotechno 6: 41.
  • Liu Y, Fan J, Gu W, Zhao Y, Zhang S, Zuo Y (2025). Genetic characterization and pathogenicity analysis of three porcine epidemic diarrhea virus strains isolated from north China. Vet Res 56: 118.
  • Liu Y, Wang X, Wang J, Zhang J, Duan C, Wang J (2022). Ergosterol peroxide inhibits porcine epidemic diarrhea virus infection in vero cells by suppressing ROS generation and p53 activation. Viruses 14: 402.
  • Lu Z, Wang X, Lin X, Mostafa S, Zou H, Wang L, Jin B (2024). Plant anthocyanins: classification, biosynthesis, regulation, bioactivity, and health benefits. Plant Physiol Bioch 217: 109268.
  • Luo X, Weng X, Bao X, Bai X, Lv Y, Zhang S, Chen Y, Zhao C, Zeng M, Huang J, Xu B, Johnson TW, White SJ, Li J, Jia H, Yu B (2022). A novel anti-atherosclerotic mechanism of quercetin: competitive binding to KEAP1 via Arg483 to inhibit macrophage pyroptosis. Redox Biol 57: 102511.
  • Macáková K, Afonso R, Saso L, Mladěnka P (2019). The influence of alkaloids on oxidative stress and related signaling pathways. Free Radical Bio Med 134: 429-444.
  • Matsumaru D, Motohashi H (2021). The KEAP1-NRF2 system in healthy aging and longevity. Antioxidnats 10: 1929.
  • Méndez-García A, García-Mendoza MA, Zárate-Peralta CP, Flores-Perez FV, Carmona-Ramirez LF, Pathak S, Banerjee A, Duttaroy AK, Paul S (2025). Mitochondrial microRNAs (mitomiRs) as emerging biomarkers and therapeutic targets for chronic human diseases. Front Genet 16: 1555563.
  • Ming X, Chen H, Yang Y, Zhao P, Sun L, Zhang C, Shin HJ, Lee JS, Jung YS, Qian Y (2022). Porcine enteric coronavirus PEDV induces the ROS-ATM and Caspase7-CAD-γH2AX signaling pathways to foster its replication. Viruses 14: 1782.
  • Mo YQ, Zhong JY, Teng MJ, Peng JC, Huang H, Aschner M, Jiang YM (2025). Advances in research on neurotoxicity and mechanisms of manganese, iron, and copper exposure, alone or in combination. J Appl Toxicol 45: 1968-1983.
  • Mukhopadhyay A, Weiner H (2007). Delivery of drugs and macromolecules to mitochondria. Adv Drug Deliver Rev 59: 729-738.
  • Narayanan KB (2025). Enzyme-based anti-inflammatory therapeutics for inflammatory diseases. Pharmaceutics17: 606.
  • Podder N, Saha A, Barman SK, Mandal S (2023). Flavonol dioxygenation catalysed by cobalt(II) complexes supported with 3N(COO) and 4N donor ligands: a comparative study to assess the carboxylate effects on quercetin 2,4-dioxygenase-like reactivity. Dalton T 52: 11465-11480.
  • Rao S, Lin Y, Lin R, Liu J, Wang H, Hu W, Chen B, Chen T (2022). Traditional Chinese medicine active ingredientsbased selenium nanoparticles regulate antioxidant selenoproteins for spinal cord injury treatment. J Nanobiotechnol 20: 278.
  • Rosales PF, Bordin GS, Gower AE, Moura S (2020). Indole alkaloids: 2012 until now, highlighting the new chemical structures and biological activities. Fitoterapia143: 104558.
  • Ross D, Siegel D (2021). The diverse functionality of NQO1 and its roles in redox control. Redox Biol 41: 101950.
  • Senel S, McClure SJ (2004). Potential applications of chitosan in veterinary medicine. Adv Drug Deliver Rev 56: 1467-1480.
  • Sharma AK, Ismail N (2025). Oxidative stress responses in ehrlichia-induced sepsis: mechanisms and implications in pathogenesis. Mbio 16: e0049925.
  • Shen J, Pan Y, Han L, Luo L, Sun T, Yu Y (2025). Nanozymes as next-generation ROS scavengers: design strategies, catalytic mechanisms, and therapeutic frontiers. J Mater Chem B 13: 8286-8297.
  • Shen N, Wang T, Gan Q, Liu S, Wang L, Jin B (2022). Plant flavonoids: classification, distribution, biosynthesis, and antioxidant activity. Food Chem 383: 132531.
  • Šimat V, Rathod NB, Čagalj M, Hamed I, Generalić Mekinić I (2022). Astaxanthin from crustaceans and their byproducts: a bioactive metabolite candidate for therapeutic application. Mar Drug 20: 206.
  • Song JH, Shim JK, Choi HJ (2011). Quercetin 7-rhamnoside reduces porcine epidemic diarrhea virus replication via independent pathway of viral induced reactive oxygen species. Viro J 8: 460.
  • Soni P, Hasan SM, Singh K, Kumar A, Ved A, Pandey M, Suvaiv N, Kushwaha SP (2025). A review on experimental and epidemiological research exploring the pathophysiology and progression of pesticide-induced diabetes mellitus. Int J Enviro Heal R 20: 1-27.
  • Suleria HA, Barrow CJ, Dunshea FR (2020). Screening and characterization of phenolic compounds and their antioxidant capacity in different fruit peels. Foods 9: 1206.
  • Su P, Jin J, Wang L, Wang J, Zhou H, Zhang Q, Xu X (2021). Porcine epidemic diarrhea virus infections induce autophagy in vero cells via ROS-dependent endoplasmic reticulum stress through PERK and IRE1 pathways. Vet Microbiol 253: 108959.
  • Sun Y, Wang L, Ma K, Shen M, Liu J, Zhang Y, Sun L (2025). Antiviral activity of 1-deoxynojirimycin extracts of mulberry leaves against porcine epidemic diarrhea virus. Animals 15: 1207.
  • Suo X, Wang J, Wang D, Fan G, Zhu M, Fan B, Yang X, Li B (2023). DHA and EPA inhibit porcine coronavirus replication by alleviating ER stress. J Viro 97: e0120923.
  • Tong T, Hu H, Zhou J, Deng S, Zhang X, Tang W, Fang L, Xiao S, Liang J (2020). Glycyrrhizic-acid-based carbon dots with high antiviral activity by multisite inhibition mechanisms. Small 16: e1906206.
  • Treml J, Šmejkal K (2016). Flavonoids as potent scavengers of hydroxyl radicals. Compr Rev Food Sci F 15: 720-738.
  • Varshosaz J (2007). The promise of chitosan microspheres in drug delivery systems. Expert Opin Drug Del 4: 263-273.
  • Vo QV, Nam PC, Thong NM, Trung NT, Phan CD, Mechler A (2019). Antioxidant motifs in flavonoids: O-H versus C-H bond dissociation. ACS Omega 4: 8935-8942.
  • Vomhof-Dekrey EE, Picklo MJ (2012). The Nrf2-antioxidant response element pathway: a target for regulating energy metabolism. J Nutr Biochem 23: 1201-1206.
  • Wan M, Xu W, Ding Y, Liu X, Fu J, Zhang P (2025). Nanozymes for regulation of reactive oxygen species in biomedical applications: design, enzyme-like activity and therapeutic mechanisms. Colloid Surface B 254: 114874.
  • Wang C, Zhang Q, Ji C, Hu Y, Yi D, Wu T, Wang L, Zhao D, Hou Y (2024). Effects of monolaurin on intestinal barrier, blood biochemical profile, immunity and antioxidant function in porcine epidemic diarrhoea virus-infected piglets. Brit J Nutr 131: 185-192.
  • Wang D, Fang L, Xiao S (2016). Porcine epidemic diarrhea in China. Virus Res 226: 7-13.
  • Wang P, Geng J, Gao J, Zhao H, Li J, Shi Y, Yang B, Xiao C, Linghu Y, Sun X, Chen X, Hong L, Qin F, Li X, Yu JS, You H, Yuan Z, Zhou D, Johnson RL, Chen L (2019). Macrophage achieves self-protection against oxidative stress-induced ageing through the mst-Nrf2 axis. Nat Commun 10: 755.
  • Wang Y, Branicky R, Noë A, Hekimi S (2018). Superoxide dismutases: Dual roles in controlling ROS damage and regulating ROS signaling. J Cell Bio 217: 1915-1928.
  • Wei MZ, Chen L, Zhang R, Chen Z, Shen YJ, Zhou BJ, Wan KG, Shan CL, Zhu EP, Cheng ZT (2024). Overview of the recent advances in porcine epidemic diarrhea vaccines. Vet J 304: 106097.
  • Wen X, Tang S, Wan F, Zhong R, Chen L, Zhang H (2024). The PI3K/akt-Nrf2 signaling pathway and mitophagy synergistically mediate hydroxytyrosol to alleviate intestinal oxidative damage. Int J Biol Sc 20: 4258-4276.
  • Wen X, Wan F, Zhong R, Chen L, Zhang H (2024). Hydroxytyrosol alleviates intestinal oxidative stress by regulating bile acid metabolism in a piglet model. Int J Mol Sci 25: 5590.
  • Wibowo JT, Ahmadi P, Rahmawati SI, Bayu A, Putra MY, Kijjoa A (2021). Marine-derived indole alkaloids and their biological and pharmacological activities. Mar Drugs 20: 3.
  • Xia M, Li M, de Souza TS, Barrow C, Dunshea FR, Suleria HA (2023). LC-ESI-QTOF-MS2 characterization of phenolic compounds in different lentil (lens culinaris M) samples and their antioxidant capacity. Front Biosci (Landmrk) 28: 44.
  • Xie H, Xiong T, Guan J, Han Y, Feng H, Xu F, Chen S, Li J, Xie Z, Liu D, Chen R (2025). Induction of mitochondrial damage via the CREB3L1/miR-34c/COX1 axis by porcine epidemic diarrhea virus infection facilitates pathogenicity. J Virol 99: e0059124.
  • Xu X, Pang Y, Fan X (2025). Mitochondria in oxidative stress, inflammation and aging: from mechanisms to therapeutic advances. Signal Transduct Tar 10: 190.
  • Xu X, Xu Y, Zhang Q, Yan F, Yin Z, Wang L, Li Q (2019). Porcine epidemic diarrhea virus infections induce apoptosis in vero cells via a reactive oxygen species (ROS)/p53, but not p38 MAPK and SAPK/JNK signalling pathways. Vet Microbiol 232: 1-12.
  • Xu Y, Wang Z, Wang Y, Yan S, Shi B (2018). Effects of chitosan as growth promoter on diarrhea, nutrient apparent digestibility, fecal microbiota and immune response in weaned piglets. J Appl Anim Res 46: 1437-1442.
  • Yang L, Ga Y, Bajpai VK, El-Kammar HA, Simal-Gandara J, Cao H, Cheng KW, Wang M, Arroo RR, Zou L, Farag MA, Zhao Y, Xiao J (2023). Advance toward isolation, extraction, metabolism and health benefits of kaempferol, a major dietary flavonoid with future perspectives. Crit Rev Food Sci 63: 2773-2789.
  • Yigit K, Chien P (2025). Lon-dependent proteolysis in oxidative stress responses. J Bacteriol 207: e0000525.
  • Yu Q, Zhan L, Wang Z, Wan Q, Sun X, Deng W, Cao X, Yu J, Xu X (2024). Anti-inflammatory oligosaccharide licensed mesenchymal stem cells allow prolonged survival of septic rats via the promotion of glutathione synthesis. Phytomedicine 135: 156173.
  • Yuan C, Huang X, Zhai R, Ma Y, Xu A, Zhang P, Yang Q (2021). In vitro antiviral activities of salinomycin on porcine epidemic diarrhea virus. Viruses 13: 580.
  • Yuan D, Guo Y, Pu F, Yang C, Xiao X, Du H, He J, Lu S (2024). Opportunities and challenges in enhancing the bioavailability and bioactivity of dietary flavonoids: a novel delivery system perspective. Food Chem 430: 137115.
  • Yuan H, Liu J, Xu R, Yang K, Qu R, Liu S, Zhang Y, Xiang M (2025). The spatiotemporal heterogeneity of reactive oxygen species in the malignant transformation of viral hepatitis to hepatocellular carcinoma: a new insight. Cell Mol Biol Lett 30: 70.
  • Zaky AA, Simal-Gandara J, Eun JB, Shim JH, Abd El-Aty AM (2021). Bioactivities, applications, safety, and health benefits of bioactive peptides from food and by-products: a review. Front Nutr 8: 815640.
  • Zambrano F, Uribe P, Schulz M, Hermosilla C, Taubert A, Sánchez R (2025). Antioxidants as modulators of NETosis: mechanisms, evidence, and therapeutic potential. Int J Mol Sci 26: 5272.
  • Zeng W, Ren J, Li Z, Jiang C, Sun Q, Li C, Li W, Li W, He Q (2022). Levistolide a inhibits PEDV replication via inducing ROS generation. Viruses 14: 258.
  • Zhang C, Liu Y, Liu X, Chen X, Chen R (2023). Comprehensive review of recent advances in chiral a-ring flavonoid containing compounds: structure, bioactivities, and synthesis. Molecules 28: 365.
  • Zhang H, Zou C, Pen O, Ashraf U, Xu Q, Gong L, Fan B, Zhang Y, Xu Z, Xue C, Wei X, Zhou Q, Tian X, Shen H, Li B, Zhang X, Cao Y (2023). Global dynamics of porcine enteric coronavirus PEDV epidemiology, evolution, and transmission. Mol Biol Evol 40: msad052.
  • Zhang S, Wang J, Liu X, Kan Z, Zhang Y, Niu Z, Hu X, Zhang L, Zhang X, Song Z (2024). Pemetrexed alleviates piglet diarrhea by blocking the interaction between porcine epidemic diarrhea virus nucleocapsid protein and ezrin. J Virol 98: e0162523.
  • Zhang YP, Yang XQ, Yu DK, Xiao HY, Du JR (2021). Nrf2 signalling pathway and autophagy impact on the preventive effect of green tea extract against alcohol-induced liver injury. J Pharm Pharmacol 73: 986-995.
  • Zhou Y, Zhang Y, Dong W, Gan S, Du J, Zhou X, Fang W, Wang X, Song H (2023). Porcine epidemic diarrhea virus activates PERK-ROS axis to benefit its replication in vero E6 cells. Vet Res 54: 9.
  • Zhou YF, Guo B, Ye MJ, Liao RF, Li SL (2016). Protective effect of rutin against H2O2-induced oxidative stress and apoptosis in human lens epithelial cells. Curr Eye Res 41: 933-942.
  • Zhu M, Lv J, Wang W, Guo R, Zhong C, Antia A, Zeng Q, Li J, Liu Q, Zhou J, Zhu X, Fan B, Ding S, Li B (2023). CMPK2 is a host restriction factor that inhibits infection of multiple coronaviruses in a cell-intrinsic manner. Plos Biol 21: e3002039.
  • Zhuang L, Zhao Y, Shen J, Sun L, Hao P, Yang J, Zhang Y, Shen Q (2025). Advances in porcine epidemic diarrhea virus research: genome, epidemiology, vaccines, and detection methods. Discov Nano 20: 48.
  • Zou H, Niu Z, Tang Z, Cheng P, Yi Y, Luo G, Huang S (2024). The mechanism of action of the active ingredients of coptidis rhizoma against porcine epidemic diarrhea was investigated using network pharmacology and molecular docking technology. Viruses 16: 1229.
  • Zou H, Wang Y, Luo G, Huang S (2025). The biomechanical phenomena observed in the cell invasion pathway of porcine epidemic diarrhea virus: a review. Arch Virol 170: 139.

Date

17.03.2026

Type

Review paper

Identifier

DOI: 10.24425/pjvs.2026.158513
×