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Abstract

The study was conducted on 26 male, 30 days-old goats, separated from their mothers, divided into two equal groups: I - control and II - experimental, consisting of 13 animals each. All animals were fed with milk replacer, experimental group received additionally 50 g/kg body weight, additive of HMB, for 60 days. The following features were analyzed: body weight, daily increases of body weight, as well as hematological and biochemical blood features. Differences in body weight were found, between experimental and control group, after 60 days of experiment 0.57 kg (p≤0.01). The daily weight gain of experimental animals was higher in comparison with control group. Significant differences were also noted in results of hematological and biochemical blood parameters. Experimental animals showed a higher level of red blood cells as well as number of lymphocytes in comparison with the control group, (p≤0.01).Significant changes were also observed in the level of triglycerides, inorganic phosphorus and protein between both groups. The acid-base balance parameters and ionogram, showed a higher pH level (p≤0.05) HCO – act., HCO – std., BE, ctCO , O sat, K+, Cl– (p≤0.01), while the anion gap (AG) and Na+ were significantly lower in control group (p≤0.01).

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Authors and Affiliations

K. Cebulska
P. Sobiech
S. Milewski
K. Ząbek
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Abstract

Orientating investigations were carried out in order to test the influence of oil extracts of lycopene (20, 40 and 60 mg/kg feed) and astaxanthin (10, 20 and 30 mg/kg feed) as feed additives on the metabolic parameters (glucose, creatinine, cholesterol) and enzyme activities (alanine aminotransferase, ALT; aspartate transaminase, AST) of laying hens. Eggs from these hens were stored at refrigerator temperatures of 4°C and 12°C for up to 30 days and analyzed for vitamin A, carotenoid and yolk color. 45 laying hens (Hy-Line W36 cross, 23 weeks of age) were divided in three groups of 15 birds each (control, lycopene fed group, astaxanthin fed group). Blood samples were taken from the hens and laid eggs were collected on days 31, 61, and 91 of the study. The eggs were stored for 30 days in refrigerators. Both lycopene and astaxanthin increased the content of glucose in serum (Р<0.05). The content of creatinine and cholesterol, and the activity of ALT, AST and alkaline phosphatase varied dose-dependently. With the exception of cholesterol, metabolite concentrations in the serum of laying hens fed different lycopene and astaxanthin doses did not exceed clinically accepted physiological levels. The carotenoid content and color of the egg yolks from laying hens fed astaxanthin was significantly higher (Р<0.05) compared to lycopene fed birds. Refrigerator storage of the eggs did not affect carotenoid content and egg yolk color compared to freshly laid eggs. Both feed additives showed a favorable effect on the metabolism of laying hens and the enrichment of egg yolks with carotenoids, astaxanthin significantly more (Р<0.05) than lycopene.
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Authors and Affiliations

L.V. Shevchenko
1
O.M. Iakubchak
1
V.A. Davydovych
1
V.V. Honchar
1
M. Ciorga
2
J. Hartung
3
R. Kołacz
2

  1. Department of Veterinary Hygiene, National University of Life and Environmental Sciences of Ukraine, Heroiv Oborony St, 15, Kiev, Ukraine
  2. Department of Public Health Protection and Animal Welfare, Institute for Veterinary Medicine, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, Gagarina 7, Toruń, Poland
  3. University of Veterinary Medicine Hannover, Bünteweg 9, Hannover, Germany
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Abstract

The present study was conducted to evaluate the effect of humic substances on performance and selected blood biochemical parameters in turkeys. A total of twenty 6-week-old turkey hybrids (Big 6) were divided into two groups. The first group of turkeys was fed the basal diet without any supplementation of humic substances as a control group. The second group was fed the basal diet supplemented with 5 g of humic substances per kg of diet. The study lasted 35 days, until the 11th week of age of the turkeys. The addition of humic substances had no effect on the live body weight, body weight gain, feed intake, feed conversion ratio, or growth rate of turkeys. Similarly, the monitored blood biochemical parameters, except for the levels of P, Mg, and Na, were not significantly affected by the addition of humic substances. The addition of humic substances led to a significant decrease in the concentration of P (p˂0.05), Mg (p˂0.05), and Na (p˂0.01) in the blood serum of turkeys. It was concluded that the addition of humic substances may lead to a reduction of some mineral substances in the blood of animals, which may be due to their chelating effects. This may indicate a reduced availability of some biologically important minerals for the body, which could subsequently manifest itself in some health issues and a decrease in animal production. During the duration of our study, there were no signs of disturbance in the health of the animals. In view of the achieved results, further studies will be necessary to determine the appropriate concentration and duration of administration of the monitored preparation and to evaluate its influence on the availability of nutrients and feed conversion and its possible use as a safe non-antibiotic growth stimulator in turkey nutrition.
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Authors and Affiliations

Hreško Šamudovská
1
L. Bujňák
1
A. Marcin
1
T. Mihok
1
M. Harčárová
1
L. Zábranský
2
P. Naď
1

  1. Department of Animal Nutrition and Husbandry, University of Veterinary Medicine and Pharmacy in Kosice, Komenskeho 73, 041 81 Kosice, Slovak Republic
  2. Department of Animal Husbandry Sciences, Faculty of Agriculture and Technology, University of South Bohemia in Ceske Budejovice, Studentska 1668, 370 05 Ceske Budejovice, Czech Republic

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